Rats were anaesthetised with isoflurane (5% induction, 2–2.5% maintenance) in 50:50% N2:O2. Incision sites were injected subcutaneously with 2 mg/kg 0.05% Bupivacaine (Pfizer, Sydney, Australia). Core body temperature was regulated and maintained at 37°C via a thermocouple rectal probe (RET-2, Physitemp Instruments Inc., Clifton, NJ, United States) and heat mat. Blood gases and pH were measured in a fast blood analyser (i-STAT 1; Abbott, Australia) at baseline prior to stroke and prior to Evans blue dye infusion from 0.1 mL blood samples taken from a femoral arterial line. This line was also used for arterial blood pressure monitoring. Prior to recovery, an additional Bupivacaine injection (0.3 mL, 0.05%, subcutaneous) and rectal paracetamol (250 mg/kg; GlaxoSmithKline, Brentford, United Kingdom) were administered for overnight pain relief. Saline was administered intraperitoneally (2 × 1.5 mL) to replace fluid loses. Following surgery, animals were returned to their cages with free access to food and water. Cages were placed half over a heat mat to allow animals to thermoregulate during recovery.
Bupivacaine
Manufactured by GlaxoSmithKline
Sourced in United Kingdom
Bupivacaine is a local anesthetic medication used to numb specific areas of the body. It is a long-acting agent that can provide prolonged pain relief. Bupivacaine is commonly used in surgical procedures, labor and delivery, and for the management of chronic pain.
Automatically generated - may contain errors
2 protocols using bupivacaine
1
Anesthetic Management for Rodent Stroke Model
2
Anesthesia and Perioperative Care for Rat Stroke
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Rats were anaesthetised with iso urane (5% induction, 2-2.5% maintenance) in 50:50% N 2 :O 2 . Incision sites were injected subcutaneously (s.c.) with 2 mg/kg 0.05% Bupivacaine (P zer, Sydney, Australia). Core body temperature was regulated via a thermocouple rectal probe (RET-2, Physitemp Instruments Inc, Clifton, New Jersey, USA) and heat mat. Blood gases were monitored on day 0 prior to stroke and day 1 prior to laminectomy from 0.1 ml blood samples from a femoral arterial line. This line was also used for arterial blood pressure monitoring. Prior to recovery, an additional Bupivacaine injection (0.3 ml, 0.05%, s.c.) and rectal paracetamol (250 mg/kg; GlaxoSmithKline, Brentford, UK) were administered for overnight pain relief. Saline was administered intraperitoneally (2 × 1.5 mL) to replace uid loses. Following surgery, animals were returned to their cages with free access to food and water. Cages were placed half over a heat mat to allow animals to thermoregulate during recovery.
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