Hek 293

Manufactured by Gemini Bio

The HEK-293 is a cell line derived from human embryonic kidney cells. It is a commonly used cell line for the expression and production of recombinant proteins in cell culture.

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Lab products found in correlation

Fetal bovine serum (fbs), by Gemini Bio (2 mentions) Blasticidin s, by InvivoGen (1 mentions) Hek293 cell, by American Type Culture Collection (1 mentions) Hygromycin b, by Cayman Chemical (1 mentions) Cycloheximide (chx), by Cayman Chemical (1 mentions) Hct116, by American Type Culture Collection (1 mentions) Hek293, by American Type Culture Collection (1 mentions)

2 protocols using hek 293

Cell Lines Characterization and Maintenance

Cited 4 times

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HEK-293
cells were obtained
from the American Type Culture Collection (ATCC, Manassas, VA). HEK-293
derivative lines 293pTP and 293 RAPA cells were previously characterized.^{55 (link),56 (link)} Mouse mesenchymal stem cell (MSC) line iMEFs was previously characterized.^{57 (link),58 (link)} All cell lines were maintained in DMEM supplemented with 10% fetal
bovine serum (FBS, Gemini Bio-Products, West Sacramento, CA), containing
100 U/mL penicillin and 100 μg/mL streptomycin, at 37 °C
in 5% CO₂ as described.^{21 (link),59 (link),60 (link)} All restriction endonuclease enzymes were purchased
from New England Biolabs (NEB, Ipswich, MA). Blasticidin S was purchased
from InvivoGen (San Diego, CA). Unless indicated otherwise, other
reagents were purchased from Sigma-Aldrich (St. Louis, MO) or Thermo
Fisher Scientific (Waltham, MA).

Wang X., Yuan C., Huang B., Fan J., Feng Y., Li A.J., Zhang B., Lei Y., Ye Z., Zhao L., Cao D., Yang L., Wu D., Chen X., Liu B., Wagstaff W., He F., Wu X., Luo H., Zhang J., Zhang M., Haydon R.C., Luu H.H., Lee M.J., Moriatis Wolf J., Huang A., He T.C, & Zeng Z. (2019). Developing a Versatile Shotgun Cloning Strategy for Single-Vector-Based Multiplex Expression of Short Interfering RNAs (siRNAs) in Mammalian Cells. ACS Synthetic Biology, 8(9), 2092-2105.

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Cell Culture Protocols for Cancer Research

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Human colorectal cancer line HCT116 and HEK-293 were purchased from the American Type Culture Collection (ATCC, Manassas, VA). 293pTP and RAPA cells derived from HEK-293 cells as previously characterized.40 (link), 41 (link) All cell lines were maintained in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Gemini Bio-Products, West Sacramento, CA), containing 100 U/ml penicillin and 100 μg/ml streptomycin, at 37 °C in 5% CO₂ as described.42 (link), 43 , 44 (link) Cycloheximide (CHX) and hygromycin B were purchased from Cayman Chemical (Ann Arbor, MI). Unless indicated otherwise, other reagents were purchased from Sigma–Aldrich (St. Louis, MO) or Thermo Fisher Scientific (Waltham, MA).

Zeng Z., Huang B., Huang S., Zhang R., Yan S., Yu X., Shu Y., Zhao C., Lei J., Zhang W., Yang C., Wu K., Wu Y., An L., Ji X., Gong C., Yuan C., Zhang L., Liu W., Feng Y., Zhang B., Dai Z., Shen Y., Wang X., Luo W., Haydon R.C., Luu H.H., Zhou L., Reid R.R., He T.C, & Wu X. (2018). The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs. Genes & Diseases, 5(1), 62-74.

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