Cronobacter sakazakii strains (strain no. ATCC29544, ATCC12868, ATCC29004, and ATCCBAA-894) and human brain microvascular endothelial cells (HBMEC) were purchased from the American Type Culture Collection (ATCC). Bacterial strains were grown at 37°C in brain heart infusion (BHI) broth (Landbridge, Beijing, China) overnight. HBMEC were grown in DMEM (Dulbecco’s modified eagle medium, Gibco, Grand Island, NY, United States) with 10% FBS (Biological Industries, Kibbutz Beit Haemek, Israel) at 37°C and 5% CO2 for the indicated period of time in different experiments.
Atccbaa 894
Manufactured by American Type Culture Collection
ATCCBAA-894 is a laboratory equipment product offered by American Type Culture Collection. It serves as a core function for general laboratory use.
Automatically generated - may contain errors
Lab products found in correlation
Atcc12868, by American Type Culture Collection (2 mentions)
Atcc 29004, by American Type Culture Collection (2 mentions)
Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions)
Hbmec, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Atcc 29544, by American Type Culture Collection (1 mentions)
Hbmec, by American Type Culture Collection (1 mentions)
Agno3, by Sinopharm (1 mentions)
Glycerol, by Sangon (1 mentions)
2 protocols using atccbaa 894
1
Cronobacter sakazakii Infection of HBMEC
2
Synthesis and Characterization of Silver Nanoparticles
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The silver to produce AgNP was from silver nitrate (AgNO 3 , Sinopharm Chemical Reagent Co. Ltd., Shanghai, China), using citrate sodium as the reducing agent (Fuchen Chemical Reagent Factory, Tianjin, China); glycerol and PVP (Sangon Biotech Co. Ltd., Shanghai, China) were used as stabilizers. All chemicals were of analytical grade. Four C. sakazakii strains: ATCC 29544 T , ATCC BAA894, ATCC 29004, and ATCC 12868, were purchased from American Type Culture Collection (ATCC, Manassas, VA) and preserved in our laboratory at -80°C. Each strain was inoculated into 20 mL of Luria-Bertani (LB) medium and incubated for 12 h at 37°C with shaking at 160 rpm.
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