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Xt2000v

Manufactured by Sysmex
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Sourced in United States

The Sysmex XT2000V is a automated hematology analyzer designed for clinical laboratory use. It is capable of analyzing various blood parameters, including red blood cells, white blood cells, and platelets. The XT2000V utilizes flow cytometry technology to provide accurate and reliable results.

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Lab products found in correlation

Cobas integra 400 plus analyzer, by Roche (1 mentions) Sta compact analyzer, by Diagnostica Stago (1 mentions) Facs lysis buffer, by BD (1 mentions) Fortessa flow cytometer, by BD (1 mentions) Trucount tube, by BD (1 mentions) Facsdiva, by BD (1 mentions)

3 protocols using xt2000v

1

Comprehensive Blood Analysis Protocol

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Complete blood counts, including leukocyte differentials (CBC/diff), were determined from blood samples collected in EDTA-coated blood tubes and analyzed using a Sysmex XT2000V (Sysmex America, Mundelein, IL). Biochemical analyses of serum samples collected in red-top Vacutainers were performed using the Roche COBAS Integra 400 plus analyzer (Roche Diagnostics, Indianapolis, IN). For coagulation studies, blood was collected in 3.2% sodium citrate-coated vacutainers and processed according to the manufacturer’s recommendations. Plasma from the citrate-coated tubes was analyzed on a STA Compact analyzer (Diagnostica Stago, Parsippany, NJ) for activated partial thromboplastin time (aPTT) and prothrombin time (PT) and for fibrinogen and D-dimer concentrations.
Wahl-Jensen V., Johnson J.C., Lauck M., Weinfurter J.T., Moncla L.H., Weiler A.M., Charlier O., Rojas O., Byrum R., Ragland D.R., Huzella L., Zommer E., Cohen M., Bernbaum J.G., Caì Y., Sanford H.B., Mazur S., Johnson R.F., Qin J., Palacios G.F., Bailey A.L., Jahrling P.B., Goldberg T.L., O’Connor D.H., Friedrich T.C, & Kuhn J.H. (2016). Divergent Simian Arteriviruses Cause Simian Hemorrhagic Fever of Differing Severities in Macaques. mBio, 7(1), e02009-15.
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2

Comprehensive Immune Cell Profiling of NHP Blood

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Complete blood cell differential count (CBC/diff) was determined from blood samples collected in ethylenediaminetetraacetic acid (EDTA)-coated blood tubes and analyzed using a Sysmex XT2000V™ (Sysmex America, IL USA).
To determine absolute numbers of peripheral blood mononuclear cells (PBMCs), EDTA whole blood samples were collected from NHPs and analyzed using TruCount™ tubes (BD Biosciences, San Jose, CA). A commercial antibody cocktail containing markers for CD45, CD3, CD4, CD8, CD14, CD16, CD20 (BD Biosciences, CA, USA), and CD159(NKG2a) (Beckman Coulter, CA, USA) was brought up to a volume of 50 µL in phosphate buffered saline (PBS) plus 2% fetal bovine serum (FBS) and 50 µL of cocktail was transferred into a TruCount™ tube. 50 µL of EDTA-anticoagulated whole blood was then added to the tube, gently mixed by vortex and incubated for 20 min at room temperature. Samples were lysed and fixed using BD FACS Lysis Buffer (BD Biosciences CA, USA) and then analyzed on the BD Fortessa Flow Cytometer. Data were analyzed using BD FACSDiva software v6.1.3 (BD Biosciences, CA, USA). Cells were first gated on CD45, then CD3 + and CD3 − populations were selected against CD45. CD3 + populations were then gated against CD4 and CD8 subpopulations. CD3- populations were gated against CD14, NKG2a and CD20 subpopulations.
Johnson R.F., Hammoud D.A., Lackemeyer M.G., Yellayi S., Solomon J., Bohannon J.K., Janosko K.B., Jett C., Cooper K., Blaney J.E, & Jahrling P.B. (2015). Small particle aerosol inoculation of cowpox Brighton Red in rhesus monkeys results in a severe respiratory disease. Virology, 481, 124-135.
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3

Comprehensive Blood Cell Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Complete blood cell differential count (CBC/diff) was determined from blood samples collected in ethylenediaminetetraacetic acid (EDTA)-coated blood tubes and analyzed using a Sysmex XT2000V™ (Sysmex America, Mundelein, IL). Serum chemistry values were assayed using the Piccolo CMP panel.
Johnson R.F., Bagci U., Keith L., Tang X., Mollura D.J., Zeitlin L., Qin J., Huzella L., Bartos C.J., Bohorova N., Bohorov O., Goodman C., Kim D.H., Paulty M.H., Velasco J., Whaley K.J., Johnson J.C., Pettitt J., Ork B.L., Solomon J., Oberlander N., Zhu Q., Sun J., Holbrook M.R., Olinger G.G., Baric R.S., Hensley L.E., Jahrling P.B, & Marasco W.A. (2016). 3B11-N, a monoclonal antibody against MERS-CoV, reduces lung pathology in rhesus monkeys following intratracheal inoculation of MERS-CoV Jordan-n3/2012. Virology, 490, 49-58.
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