The following cancer cell lines were obtained from the American Type
Culture Collection (Manassas, VA, USA): SK-BR-3, SK-OV-3, MDA-MB-453, and
MDA-MB-468. SK-BR-3 and SK-OV-3 cells were cultured in McCoy’s 5a medium,
whereas MDA-MB-453 and MDA-MB-468 cells were cultured in RPMI1640 medium. The
breast cancer cell line JIMT-1 was obtained from AddexBio and cultured in DMEM
medium. The HCC1954 cell line was a generous gift from Drs. Adi Gazdar, John
Minna, and Kenneth Huffman (Hamon Center for Therapeutic Oncology Research,
University of Texas Southwestern Medical Center at Dallas) and was cultured in
RPMI1640 medium. All media were supplemented with 1% penicillin/streptomycin, 1%
GlutaMAX (Thermo Fisher Scientific, Waltham, MA, USA), 1% sodium pyruvate and
10% fetal calf serum. The cell lines were tested monthly for mycoplasma
contamination and were authenticated annually at the University of Arizona
Genetics Core through DNA fingerprint analysis.
Culture Collection (Manassas, VA, USA): SK-BR-3, SK-OV-3, MDA-MB-453, and
MDA-MB-468. SK-BR-3 and SK-OV-3 cells were cultured in McCoy’s 5a medium,
whereas MDA-MB-453 and MDA-MB-468 cells were cultured in RPMI1640 medium. The
breast cancer cell line JIMT-1 was obtained from AddexBio and cultured in DMEM
medium. The HCC1954 cell line was a generous gift from Drs. Adi Gazdar, John
Minna, and Kenneth Huffman (Hamon Center for Therapeutic Oncology Research,
University of Texas Southwestern Medical Center at Dallas) and was cultured in
RPMI1640 medium. All media were supplemented with 1% penicillin/streptomycin, 1%
GlutaMAX (Thermo Fisher Scientific, Waltham, MA, USA), 1% sodium pyruvate and
10% fetal calf serum. The cell lines were tested monthly for mycoplasma
contamination and were authenticated annually at the University of Arizona
Genetics Core through DNA fingerprint analysis.