Dulbecco s modified eagle s medium

Manufactured by National Collection of Authenticated Cell Cultures

Sourced in United States

Dulbecco's Modified Eagle's Medium (DMEM) is a widely used cell culture medium formulation. It provides essential nutrients and growth factors to support the in vitro cultivation of various cell types. DMEM is a complete and balanced medium that maintains the physiological pH and osmolarity suitable for cell growth and proliferation.

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Lab products found in correlation

Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Biu87, by National Collection of Authenticated Cell Cultures (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Mccoy s 5a medium, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by National Collection of Authenticated Cell Cultures (1 mentions)

2 protocols using dulbecco s modified eagle s medium

Bladder Cell Culture Protocol

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Primary cultures of normal bladder urothelial cells (NBUCs) were established from fresh specimens of patients as described previously. 14 Human bladder cancer cell lines, including BIU-87, J82, 5637, EJ, and RT4 were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA).The UM-UC-3 cell line was cultured in Dulbecco's Modified Eagle's Medium was obtained from the Cell Bank of the Chinese Academy of Sciences and cultured in McCoy's 5a Medium supplemented with 10% FBS (Gibco).

Yang C., Zhang W., Wang L., Kazobinka G., Han X., Li B, & Hou T. (2016). Musashi-2 promotes migration and invasion in bladder cancer via activation of the JAK2/STAT3 pathway. Laboratory investigation; a journal of technical methods and pathology, 96(9).

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Culturing RBE Cells in DMEM

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RBE cells (a human CCA cell line) were purchased from the cell bank of the Chinese Academy of Sciences (Shanghai, People’s Republic of China) and cultured with Dulbecco’s Modified Eagle’s Medium containing 10% fetal bovine serum and 100 U/mL penicillin/streptomycin at 37°C in humidified 5% CO₂. The above three reagents were purchased from Gibco (New York, NY, USA).

Zhang D.Y., Liu Z., Lu Z., Sun W.L., Ma X., Zhang P., Wu B.Q, & Cui P.Y. (2018). Lentivirus-mediated overexpression of HSDL2 suppresses cell proliferation and induces apoptosis in cholangiocarcinoma. OncoTargets and therapy, 11, 7133-7142.

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