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2 protocols using n hexane hplc grade

1

Encapsulation of D-limonene in Alginate

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D-limonene was obtained from HiMedia Laboratories Pvt.Ltd (Mumbai, India). Sodium alginate (from Macrocystis pyrifera, molecular weight: 80,000e120,000. M/G ratio: 1.56) was purchased from Sigma (St. Louis, USA). Calcium chloride dihydrate was purchased from Acros Organics (New Jersey, USA), while n-hexane (HPLC grade) was suplied from Carlo Erba Reagenti SpA (Rodano, Italy). All other chemicals were of analytical reagent grade and they were used without any further purification.
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2

HPLC Analysis of Bioactive Compounds

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Hydrochloric acid, n-hexane (HPLC grade) and Folin-Ciocalteu reagent were purchased from Carlo Erba Reagents (Val de Reuil, France), and sodium hydroxide and sodium nitrite from Gram-mol (Zagreb, Croatia), ferric chloride hexahydrate and sodium acetate trihydrate from T.T.T. (Sveta Nedelja, Croatia), and aluminum chloride hexahydrate from Kemika (Zagreb, Croatia). Sodium carbonate was supplied by Panreac Química (Castellar del Vallès, Spain), ferrous sulfate heptahydrate by Acros Organics (Madrid, Spain) and 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ) by Alfa Aesar (Karlsruhe, Germany). HPLC grade standards of theobromine, caffeine and (+)-catechin, (−)-epicatechin gallate, caffeic acid and p-coumaric acid, gallic acid and (−)-epicatechin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methanol (J.T. Baker, Deventer, The Netherlands) and formic acid (Scharlau Chemie, Spain) used for mobile phase preparation were HPLC grade.
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