Lc3b ab

Manufactured by Cell Signaling Technology

Sourced in United States

LC3B Ab is a primary antibody that specifically recognizes the LC3B protein, a widely used marker for the process of autophagy. This antibody is designed for use in various immunodetection techniques, such as Western blotting, immunohistochemistry, and immunofluorescence.

Automatically generated - may contain errors

Lab products found in correlation

Immunohistochemistry kit, by Roche (1 mentions) Cd34 pe, by BD (1 mentions) C kit apc, by BD (1 mentions) Ly6g apc, by BD (1 mentions) Alexa fluor secondary antibody, by Thermo Fisher Scientific (1 mentions) Horseradish peroxidase conjugated secondary antibody, by Rockland Immunochemicals (1 mentions) Sca 1 fitc, by BioLegend (1 mentions) Cxcr4 fitc, by BD (1 mentions) Sca1 apc, by BioLegend (1 mentions)

2 protocols using lc3b ab

Immunohistochemical Analysis of LC3B

Check if the same lab product or an alternative is used in the 5 most similar protocols

Heart sections from the Wistar rats were stained through the use of an immunohistochemistry kit (no.760-700, Roche Life Science, Indianapolis, USA), and the target protein was identified by brown color. As primary antibodies, LC3B Ab (#2775, Cell Signaling Technology, Maryland, USA) were used.

Lai C.H., Tsai C.C., Kuo W.W., Ho T.J., Day C.H., Pai P.Y., Chung L.C., Huang C.C., Wang H.F., Liao P.H, & Huang C.Y. (2016). Multi-Strain Probiotics Inhibit Cardiac Myopathies and Autophagy to Prevent Heart Injury in High-Fat Diet-Fed Rats. International Journal of Medical Sciences, 13(4), 277-285.

+ Open protocol

+ Expand

Antibodies for Western Blot, Immunofluorescence, and Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following primary antibodies were used for western blot and immunofluorescence imaging: DNM2 Ab, Santa Cruz (#sc-6400, #C-18; Dallas, TX); and CXCR4 Ab, Santa Cruz (#sc-9046, H-118). The following secondary antibodies were used: horseradish peroxidase–conjugated secondary antibodies were either from Rockland (Limerick, PA) or from Santa Cruz; Alexa Fluor secondary antibodies were from Thermo Fisher Scientific. The following antibodies were used for immunohistochemistry: Ly6G Ab, Cell Signaling (#87048; Danvers, MA), BCL-6 Ab, Abcam (#ab243150; Waltham, MA), and LC3B Ab, Cell Signaling (#83506). The following antibodies were used for flow cytometry experiments: CXCR4-FITC, BD Biosciences (#561735; Franklin Lake, NJ), and Ly6G-APC, BD Biosciences (#560599), c-kit APC, BD Pharmingen (Franklin Lake, NJ, #553356), IL7R BV421 (#135023; Biolegend), CD34-PE (#551387; BD Pharmingen), CD16/32-PE-Cy7 (#25-0161-82; ThermoFisher), Sca-1-FITC (#122505; Biolegend). In Cyto-ID autophagy experiments, Sca-1 APC (#108112; Biolegend) was used.

Willis A.J., Corey S.J., Murga-Zamalloa C., Karimi S.S., Khaddour K., Quigley J., Eklund E.A, & Chen Y. (2022). Dynamin-2 deficiency causes age- and sex-dependent neutropenia and myelodysplasia in mice. Blood Advances, 7(8), 1418-1431.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!