Faecal samples were collected by the participants using a nylon flocked ESwab 480CE (Copan, Brescia, Italy). The swab was spread onto ChromID ESBL agar (BioMerieux, Marcy l’Etoile, France). To confirm an ESBL phenotype, combinatory Etests (BioMerieux) with a cephalosporin ± an inhibitor was used.
Eswab 480ce
Manufactured by Copan
Sourced in Italy
The ESwab 480CE is a transport medium designed for the collection, preservation, and transport of clinical specimens. It features a flocked swab and a liquid Amies-based medium to maintain the viability of aerobic and anaerobic bacteria, as well as certain viruses, during transportation to the laboratory.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using eswab 480ce
1
Detecting ESBL-producing Bacteria in Feces
2
ESBL Bacterial Identification Protocol
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The fresh stool samples were collected by the subjects using a nylon flocked ESwab 480CE (Copan, Brescia, Italy), and then the swab was spread onto the ChromID ESBL agar (BioMerieux, Marcy l’Etoile, France). After the incubation at 37.8∘C for 24 h, the confirmation of ESBL phenotype was carried out by the disk diffusion method according to the recommendation of CLSI (2011) . The positive isolates were further identified using conventional biochemical tests and the API 20E system (Sysmex-bioMerieux, Tokyo, Japan).
3
Porcine Tonsil Sampling for Microbiome
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From each farm, samples were taken from ten clinically healthy grower pigs from the same batch, if possible from one pig per pen. The pigs were 8–13 weeks of age and had not been subjected to any treatment for at least 1 month before sampling. The pigs had all been weaned at between 4 and 6 weeks of age [25 ]. A sample was obtained from each pig’s palatine tonsils by opening the mouth using snares of braided nylon rope around the upper and lower jaws and rubbing an eSwab™ 480CE (Copan Diagnostics, Inc., Corona, CA, USA) on the tonsillar surface for 3 s. The swabs were immediately placed in tubes containing liquid Amies transport medium, transported to the laboratory at ambient temperature, and were processed for bacteriological analysis within 18 h of sampling.
4
Amniotic Fluid Analysis for MIAC and IAI
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Women participating in the study underwent amniocentesis and amniotic fluid was aspirated under sterile conditions through an ultrasound guided transabdominal amniocentesis. After amniocentesis, approximately 3.5 mL of amniotic fluid was used for the detection of MIAC and assessment of IL-6 levels for the detection of IAI. The remaining amniotic fluid was further processed, where samples were aliquoted and stored either as non-centrifuged, or as centrifuged for 15 min at 2000 g at 4 • C to remove cells and debris. In the latter cases, supernatant was aspirated and further divided into aliquots while pellets were left in the original polypropylene tubes. All aliquots were stored at -80 • C until analysis. After delivery, the chorionic and amniotic membrane were separated approximately 10 cm from the initial rupture of the membranes and samples were collected using Charcoal swab and nylon swabs (E-swab 480CE, Copan, Italy).
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