Orion 420a

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Orion 420A+ is a benchtop pH/mV/ISE meter designed for accurate measurements in laboratory settings. It features a large, easy-to-read LCD display and provides stable, reliable readings. The device supports a wide range of pH, mV, and ion-selective electrode (ISE) measurements.

Automatically generated - may contain errors

Lab products found in correlation

7 protocols using orion 420a

Analyzing pH and Color of Cooked Pork Sausages

Check if the same lab product or an alternative is used in the 5 most similar protocols

For pH of the cooked pork sausages, a 10 g sausage and 90 mL DDW were mixed and homogenized. Duplicate readings per sample were obtained using a pH meter (Orion 420A+, Thermo Electron Co., USA). The average value of readings was reported, and the pH meter was calibrated with standard buffers at pH 4.0 and 10.0 on a daily basis.
To determine the objective color space values of the pork loin sausages, each sample sausage was cut into 2 cm sections and bloomed for 10 min. Individual CIE L* (lightness), a* (redness), and b* (yellowness) color space values were then determined using a colorimeter (Minolta Chroma Meter CR-300, Minolta Co., Ltd., Ramsey, NJ), which was calibrated daily with a white tile (Y=94.3, x=0.3130 and y=0.3199). Two different readings were taken per sample, and the average of CIE L*, a*, and b* was reported.

Ryu K.S., Shim K.S, & Shin D. (2014). Effect of Grape Pomace Powder Addition on TBARS and Color of Cooked Pork Sausages during Storage. Korean Journal for Food Science of Animal Resources, 34(2), 200-206.

+ Open protocol

+ Expand

Physicochemical Characterization of LEO

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ten g of LEO and 90 mL of double distilled water were homogenized for 30 s at 13,500 rpm (T25B, IKA Sdn. Bhd., Malaysia). Duplicate readings of each sample were taken using a pH meter (Orion 420A+, Thermo Electron Co., USA), and the average pH value per treatment was reported.
A colorimeter (Minolta Chroma Meter CR-300, Minolta Co. Ltd, USA) was used to determine CIE L* (lightness), a* (redness) and b* (yellowness) color space values. The colorimeter was calibrated daily using a white tile (Y=92.8, x=0.3134, y=0.3193), and duplicate readings per sample were conducted daily. The average of each color space value was reported as CIE L*, a*, and b* color space values, respectively.
Individual water activity (a_w) of LEO was determined for each treatment using a Thermoconstanter a_w Sprint Novasina TH500 (Novasina, Axair Ltd., Switzerland) at 25℃, and the average of nine different LEO a_w readings per treatment was reported.

Choe H.S., Shim K., Jung J.H., Chung Y.H, & Shin D. (2014). Effects of Ripening Conditions on the ‘Lomo embuchado’ Sausage Quality. Korean Journal for Food Science of Animal Resources, 34(3), 333-338.

+ Open protocol

+ Expand

Electrochemical Detection of Neurotransmitters

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dopamine and 2-aminophenol were purchased from Alfa Aesar (Ward Hill, MA, USA). Sodium phosphate monobasic was received from Fisher Scientific (Waltham, MA, USA) and catechol was obtained from Acros (Geel, Belgium). All chemicals were used as received. The background electrolyte (10 mM phosphate buffer) was prepared weekly by dissolving the desired amount of solid NaH₂PO₄. The pH of the solutions was adjusted, when necessary, using either 1 mol·L⁻¹ NaOH or 1 mol·L⁻¹ HCl (Fisher Scientific) and measured using a glass electrode and a digital pHmeter (Orion 420A+, Thermo; Waltham, MA). Stock solutions of Dopamine, 2-aminophenol and catechol (10 mM each) were prepared by dissolving the desired amount of each compound in ultrapure water (≥ 18 MΩ·cm, NANOpure Diamond, Barnstead; Dubuque, Iowa). Working solutions were prepared by diluting the stock solutions in 10 mM phosphate buffer. Standard-grade PMMA plates (150 × 70 × 1.5 mm) were purchased from Gravograph (Duluth, GA, USA) and used to produce the microdevices herein described. Sylgard 184 silicone elastomer and curing agent were obtained from Dow Corning. Sugar, used for the formation of the PDMS sponge (decoupler), was food-grade and acquired in a local grocery store.

Gabriel E.F., Coltro W.K, & Garcia C.D. (2014). Fast and Versatile Fabrication of PMMA Microchip Electrophoretic Devices by Laser Engraving. Electrophoresis, 35(16), NA.

+ Open protocol

+ Expand

Preparation of Buffered Metal Solutions

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sulfuric acid (ACS/FCC, BDH Aristar, 95.0–98.0%) was acquired from VWR (Pittsburgh, PA, USA). Sodium bicarbonate and hexa-ammine ruthenium(iii) chloride from Sigma-Aldrich (St. Louis, MO, USA). Copper(ii) sulfate, copper(ii) chloride, copper(ii) acetate, cobalt(ii) acetate, and silver nitrate were purchased from Fisher Scientific (Fair Lawn, NJ, USA). All aqueous solutions were prepared using 18 MΩ cm water (NANOpure Diamond, Barnstead; Dubuque, IA) and analytical reagent grade chemicals. Phosphate buffer solution was prepared by dissolving anhydrous Na₂HPO₄ (Fisher Scientific; Fair Lawn, NJ, USA) in ultrapure water. The pH of the solutions was measured using a glass electrode connected to a digital pH meter (Orion 420A+, Thermo; Waltham, MA, USA) and adjusted with 1 mol L⁻¹ solutions of either NaOH or HCl.

Gomez F.J., Chumanov G., Silva M.F, & Garcia C.D. (2019). CO2 reduction using paper-derived carbon electrodes modified with copper nanoparticles. RSC Advances, 9(58), 33657-33663.

+ Open protocol

+ Expand

Glucose Oxidase Activity Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

All chemicals were analytical-reagent grade and used as received. Hydrogen peroxide (35%) was purchased from Columbus Chemical (Columbus, WI). Sodium acetate was purchased from Mallinckrodt Baker, Inc. (acquired by Avantor Performance Materials, Center Valley, PA). Hydrochloric acid and sodium hydroxide were purchased from EMD Millipore (Billerica, MA). Block copolymers of polystyrene and poly-(2-vinylpyridine) (MW_PS = 101,000 Da, MW_P2VP = 29,000 Da, M_w/M_n = 1.6) were purchased from Polymer Source Inc. (Dorvel, Quebec). Citric acid, ethanol, GOx from Aspergillus niger (17.3 U·mg−1), horseradish peroxidase (HRP, 199 purpurogallin units mg−1), N,N-dimethylformamide (DMF), o-xylene, PS (MW = 95,800 Da), and α-D-Glucose were purchased from Sigma Aldrich (St. Louis, MO). O-dianisidine dihydrochloride was purchased from TCI America (Portland, OR) and sulfuric acid was purchased from VWR (Radnor, PA). The pH of the aqueous solutions was adjusted using either 1 M NaOH or 1 M HCl and measured using a glass electrode and a digital pH meter (Orion 420A+, Thermo; Waltham, MA). All aqueous solutions were prepared using 18 MΩcm water (NANOpure Diamond, Barnstead; Dubuque, IA). Unless otherwise stated, experiments were conducted at room temperature (22 ± 2 °C).

Bhakta S.A., Benavidez T.E, & Garcia C.D. (2014). Immobilization of Glucose Oxidase to Nanostructured Films of Polystyrene-block-poly(2-vinylpyridine). Journal of colloid and interface science, 430, 351-356.

+ Open protocol

+ Expand

Enzymatic Uric Acid Determination

Check if the same lab product or an alternative is used in the 5 most similar protocols

Recombinant uricase (also known as urate oxidase) from Candida sp. expressed in E. coli and potassium hexacyanoferrate (III) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Uric acid, 99% was obtained from Alfa Aesar (Ward Hill, MA, USA). SulfUric acid (ACS/FCC, BDH ARISTAR, 95.0-98.0 %) was acquired from VWR (Pittsburgh, PA, USA). All aqueous solutions were prepared using 18 MΩ·cm water (NANOpure Diamond, Barnstead; Dubuque, IA) and analytical reagent grade chemicals. Phosphate buffer solution was prepared by dissolving anhydrous Na₂HPO₄ (Fisher Scientific; Fair Lawn, NJ, USA) in ultrapure water. The pH of the solutions was measured using a glass electrode connected to a digital pH meter (Orion 420A+, Thermo; Waltham, MA, USA) and adjusted with 1 mol·L⁻¹ solutions of either NaOH or HCl. Standard Uric acid solution was freshly prepared in phosphate buffer solution (10 mM, pH = 8.5) before each experiment.

Giuliani J.G., Benavidez T.E., Duran G.M., Vinogradova E., Rios A, & Garcia C.D. (2015). Development and Characterization of Carbon Based Electrodes from Pyrolyzed Paper for Biosensing Applications. Journal of electroanalytical chemistry (Lausanne, Switzerland), 765, 8-15.

+ Open protocol

+ Expand

Lysozyme Adsorption Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

All aqueous solutions were prepared using 18 MΩ⋅cm water (NANOpure Diamond, Barnstead; Dubuque, IA) and analytical grade chemicals. Lysozyme (LSZ, from chicken egg white) was purchased from Sigma‐Aldrich (St. Louis, MO). Sodium phosphate monobasic anhydrous was obtained from Fisher Scientific (Fair Lawn, NJ). AZ P4330‐RS Photoresist was acquired from AZ Electronic Materials USA Corp. (Somerville, NJ). Propylene glycol monomethyl ether acetate (PGMEA 99 %) was obtained from Alfa Aesar (Ward Hill, MA).
Stock solutions of LSZ (0.10 mg.mL⁻¹) were prepared daily by dissolving a known amount of protein in 10 mmol.L⁻¹ phosphate buffer solution at pH=11.00 (IEP, isoelectric point). The latter aspect is important as this pH value avoids potential contribution of (additional) charged residues on the overall dielectric behavior of proteins.[⁴², ⁴³] The pH of buffer solution was adjusted using 1 mol.L⁻¹ NaOH and measured with a glass electrode connected to a digital pH meter (Orion 420A+, Thermo; Waltham, MA). Before adsorption, the protein solution was filtered through 0.2 μm poly(tetrafluoroethylene) membrane (PTFE, VWR International; Radnor, PA) to remove any aggregates.

Benavidez T.E., Guerra J.D, & Garcia C.D. (2022). Dielectric Spectroscopy Can Predict the Effect of External AC Fields on the Dynamic Adsorption of Lysozyme. Chemphyschem, 23(10), e202100914.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!