Amphiphilic block-co-polymers PEG-P(AIE) were synthesized according to the previously reported methodology [22 (link)]. To prepare the AIE polymersomes, PEG44-P(AIE)14 was dissolved in THF (2 mg/mL) in a 4 mL glass vial with a magnetic stirring bar and sealed with a rubber septum. After stirring for 10 min, 0.5 mL of ultrapure Milli-Q water was added to the polymer solution through a syringe pump (Chemyx, Inc., Fusion 100, KR Analytical Limited, Stafford, TX, USA) with a speed of 0.25 mL/h. Then, the resulting cloudy solution was transferred into a pre-hydrated dialysis bag (Spectra/Pro®, MWCO 12,000–14,000, 2 mL/cm, Rancho Dominguez, CA, USA) for dialysis against 0 mM NaCl or 100 mM NaCl solution at 4 °C for at least 24 h. Dynamic light scattering (DLS, Nano ZSP, Malvern Instruments, Malvern, UK), scanning electron microscopy (SEM, FEI Quanta 200 3D FEG, Thermo Fisher Scientific, Waltham, MA, USA), and cryogenic transmission electron microscopy (cryo-TEM, TU/e CryoTitan, Thermo Fisher Scientific, Waltham, MA, USA) were used for size and morphological characterization. For characterization of the fluorescence, a Spark® 10M microplate reader (TECAN, Männedorf, Switzerland), and two-photon/confocal laser scanning microscopy (TP-CLSM, Leica TCS SP8X, Wetzlar, Germany) were applied.
Fei quanta 200 3d feg
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom, United States
The FEI Quanta 200 3D FEG is a scanning electron microscope (SEM) that utilizes a field emission gun (FEG) to produce high-resolution images. It is capable of operating in various vacuum modes, including high vacuum, low vacuum, and environmental modes, to accommodate a wide range of sample types. The Quanta 200 3D FEG provides users with advanced imaging and analytical capabilities.
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2 protocols using fei quanta 200 3d feg
1
Synthesis and Characterization of Amphiphilic AIE Polymersomes
2
Fabrication of Janus Polymeric Microcapsules
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CHI/Hep microcapsules
were prepared by the LbL self-assembly method.55 (link)−57 (link) Briefly, 1
mg/mL of CHI and Hep solution containing 0.1 M NaCl was alternatively
deposited on the surface of template microparticles under continuous
shaking for 15 min. Unassembled polymer was removed by washing three
times with 0.1 M NaCl. Fluorescein-labeled CHI/Hep microcapsules were
synthesized by adding one layer of rhodamine B–CHI during the
assembly process.58 (link)−60 (link) In order to construct Janus capsule motors, a droplet
of the CHI/Hep microparticle solution was dropped on a hydrophilic
silica slide to form a monolayer of microparticles. After evaporation
in air, a turbo sputter coater (Quorum Technologies, K575X) was used
to coat one side of the polymeric particles with a thin gold layer
(65 mV, 30 s). Ultrasound treatment was used to redisperse the Janus
polymeric particles into aqueous solution. Next, 50 mg/mL of NH4F solution was then used to dissolve the silica template to
form Janus polymeric capsules with hollow structure. The morphology
of JPMs was characterized with scanning electron microscopy (FEI Quanta
200, 3D FEG, ThermoFisher Scientific, USA) and confocal laser scanning
microscopy (Zeiss, LSM510 META, Germany). EDX elemental mapping analysis
of JPMs was obtained by SEM (Phenom ProX, The Netherlands).
were prepared by the LbL self-assembly method.55 (link)−57 (link) Briefly, 1
mg/mL of CHI and Hep solution containing 0.1 M NaCl was alternatively
deposited on the surface of template microparticles under continuous
shaking for 15 min. Unassembled polymer was removed by washing three
times with 0.1 M NaCl. Fluorescein-labeled CHI/Hep microcapsules were
synthesized by adding one layer of rhodamine B–CHI during the
assembly process.58 (link)−60 (link) In order to construct Janus capsule motors, a droplet
of the CHI/Hep microparticle solution was dropped on a hydrophilic
silica slide to form a monolayer of microparticles. After evaporation
in air, a turbo sputter coater (Quorum Technologies, K575X) was used
to coat one side of the polymeric particles with a thin gold layer
(65 mV, 30 s). Ultrasound treatment was used to redisperse the Janus
polymeric particles into aqueous solution. Next, 50 mg/mL of NH4F solution was then used to dissolve the silica template to
form Janus polymeric capsules with hollow structure. The morphology
of JPMs was characterized with scanning electron microscopy (FEI Quanta
200, 3D FEG, ThermoFisher Scientific, USA) and confocal laser scanning
microscopy (Zeiss, LSM510 META, Germany). EDX elemental mapping analysis
of JPMs was obtained by SEM (Phenom ProX, The Netherlands).
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