Total protein was extracted using RIPA buffer with protease inhibitors added (1:100). Nuclear protein extraction was carried out using the Active Motif Nuclear Extraction Kit. Western blotting was performed by blotting Mini-PROTEAN TGX 5–20% gradient gels (Bio-Rad) on nitrocellulose membranes using the Trans-Blot Turbo system (Bio-Rad). Blocking was carried out using 5% milk in TBST. Antibodies are listed in Supplementary Materials .
Mini protean tgx 5 20 gradient gels
Manufactured by Bio-Rad
The Mini-PROTEAN TGX 5–20% gradient gels are a line of pre-cast polyacrylamide gels for use in protein electrophoresis. These gels feature a 5–20% polyacrylamide gradient, which allows for the separation of a wide range of protein molecular weights in a single gel.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mini protean tgx 5 20 gradient gels
1
Protein Extraction and Western Blotting
2
Protein Extraction and Western Blotting
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