A CellTitre-Blue® assay (Promega, UK) was used to evaluate cell viability. Scaffolds were placed in a new 48 well suspension plate and washed 3 times in PBS, 480 µl of stock solution (5:1, media/assay) was added to each scaffold and incubated for 2 h. Fluorescence was read using a microplate reader (Modulus II 9300-062, Turner Biosystems) at Ex 520 nm Em 580–640 nm, N = 4 independent replicates. A standard curve was used to estimate cell number.
Modulus 2 9300 062
Manufactured by Promega
The Modulus II 9300-062 is a multi-mode microplate reader that can perform absorbance, fluorescence, and luminescence measurements. It is designed to support a variety of common laboratory assays.
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Lab products found in correlation
3 protocols using modulus 2 9300 062
1
CellTitre-Blue Assay for Cell Viability
2
Cell Viability Assay on Scaffolds
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CellTitre-Blue® assay (Promega, Southampton, UK) was used to determine cell viability according to manufacturer’s instruction on the day of each time point. Cell seeded scaffolds were washed 3 times in PBS and then a mixture of CellTitre-Blue® assay with cell culture media (1:5) was added on scaffolds to incubate for 3.5 h. Fluorescence was read using a microplate reader (Modulus II 9300–062, Turner Biosystems) at Ex 520 nm Em 580–640 nm.
3
Cell Viability Assay using CellTiter-Blue
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Cell metabolism was assessed using a CellTitre-Blue ® assay (Promega, UK). Scaffold were washed 3 times in PBS and 80 µl of CellTitre-Blue ® assay was added to 400 µl cell culture media (1:5) and incubated for 2 hours. Fluorescence was read using a microplate reader (Modulus II 9300-062, Turner Biosystems) at Ex 520 nm Em 580-640 nm, N ≥ 4 independent replicates.
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