Inhibitors CAY10657 (Cat# 11140, CAS № 494772-86-0) and 5Z-7-oxozeaenol,Ox1, (Cat# 17459, CAS № 253863-19-3), and U0126 (Cat#70970) were obtained from Cayman Chemical (Ann Arbor, Michigan); 5Z-7-oxozeaenol, Ox2, (Cat#3604) was obtained from Tocris Bio-Techne (Minneapolis, MN); BMS-345541 (Cat#A3248), CX-5461 (Cat# A8337), Birinapant TL-32711 were from APExBio (Houston, TX); SB202190 (Cat# 559388) was from Calbiochem (EMD Millipore; Billerica, MA); Nutlin 3A (Cat# SML0580) was obtained from Sigma Aldrich (Atlanta, GA). Antibodies for: GAPDH (Cat# sc-25778), Fibrillarin (Cat# sc-25397), and IκBα (Cat# sc-371) were from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA); RELA/p65 (Cat# 8242) and phospho-p65/RELA (Ser536) (Cat#3033); phospho-p53 (Ser15) (Cat# 9284) were from Cell Signaling Technology (Danvers, MA); α-Tubulin (Cat# T6074), Goat anti-Rabbit IgG (H + L)-Horseradish Peroxidase (HRP) (Cat# 170–6515) and goat anti-Mouse IgG (H + L)-HRP (CAT# 170–6516) secondary antibodies were from BIO-RAD Laboratories (Hercules, CA).
Cx 5461
Manufactured by Apexbio
Sourced in United States
CX-5461 is a laboratory instrument designed for high-throughput screening and analysis. It is capable of performing various spectroscopic techniques, including absorbance, fluorescence, and luminescence measurements. The CX-5461 is a versatile tool for researchers in fields such as biochemistry, molecular biology, and drug discovery.
Automatically generated - may contain errors
Lab products found in correlation
Fibrillarin, by Santa Cruz Biotechnology (1 mentions)
Goat anti mouse igg h l hrp, by Bio-Rad (1 mentions)
Cay10657, by Cayman Chemical (1 mentions)
Bms 345541, by Apexbio (1 mentions)
Birinapant tl 32711, by Apexbio (1 mentions)
Goat anti rabbit igg h l horseradish peroxidase hrp, by Bio-Rad (1 mentions)
U0126, by Cayman Chemical (1 mentions)
α tubulin, by Bio-Rad (1 mentions)
Phospho p65 rela ser536, by Cell Signaling Technology (1 mentions)
Rela p65, by Cell Signaling Technology (1 mentions)
Phospho p53 ser15, by Cell Signaling Technology (1 mentions)
Nutlin 3a, by Merck Group (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
Doxycycline, by Thermo Fisher Scientific (1 mentions)
α sma, by Abcam (1 mentions)
Antibodies for p53, by Cell Signaling Technology (1 mentions)
Pifithrin α, by Selleck Chemicals (1 mentions)
Ifn γ, by Proteintech (1 mentions)
Lipopolysaccharides (lps), by Solarbio (1 mentions)
4 protocols using cx 5461
1
Inhibitors and Antibodies for Cell Signaling
2
Optimizing Drug Treatments in Cell Cultures
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CX-5461 (ApexBio) was dissolved in 50 mM NaH2PO4 (pH 4.5) to obtain a 1 mM stock solution. 2’-C-Methyladenosine (Santa Cruz Biotechnologies) was dissolved in DMSO to obtain a 100 mM stock solution. Doxycycline (Fisher Scientific) was dissolved in H2O to obtain a 100 mM stock solution. Drugs were stored in aliquots at -20°C. Treatments were performed with drugs or vehicle diluted in medium at the indicated concentrations as described in the Results. For qRT-PCR analysis, EU incorporation, and EdU incorporation, 30-50% confluent cells were treated for 24 h. For MTT analysis, cells were treated for 3-5 days refreshing the medium every 48 hours for the duration of the treatment.
3
Molecular Effects of Therapeutic Agents in Pulmonary Hypertension
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CX‐5461 was from ApexBio Technology LLC (Houston, TX, USA). Monocrotaline (MCT, #C2401) and Sugen (SU 5416, #S8442) were purchased from Sigma‐Aldrich (St Louis, MO, USA). Pifithrin‐α was purchased from Selleck Chemicals (Houston, TX, USA). Primary antibodies used were as follows: phospho‐UBF1 (S484) (#ab182583, 1:100 for immunohistochemistry), proliferating cell nuclear antigen (PCNA) (#ab18197, 1:500 for immunofluorescence, RRID:AB_444313), α‐smooth muscle actin (α‐SMA, #ab5694, 1:200 for immunohistochemistry, RRID:AB_2223021), CD68 (#ab125212, 1:500 for immunohistochemistry, RRID:AB_10975465), CD4 (#ab203034, 1:400 for immunohistochemistry), CD8 (#ab33786, 1:200 for immunohistochemistry, RRID:AB_726709), and CD31 (#ab182981, 1:1500 for immunohistochemistry) were from Abcam (Cambridge, UK). Antibodies for p53 (#2524, 1:1000 for western blot, RRID:AB_331743) and phospho‐p53 (Ser15) (#9284, 1:1000 for western blot and 1:150 for immunohistochemistry, RRID:AB_331464) were from Cell Signaling (Beverley, MA, USA).
4
CX-5461 Inhibition of LPS/IFN-γ
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CX-5461 (purchased from ApexBio Technology LLC, Houston, TX, United States) was initially dissolved in DMSO, and diluted in the culture medium to the final concentration of 1 μM (treatment duration 24 h). DMSO was used as vehicle control. LPS was purchased from Solarbio (Beijing, China) and used at the final concentration of 1 μg/ml. IFN-γ was from Proteintech (Wuhan, Hubei Province, China) and used at 150 U/ml.
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