Lsm 780 confocal microscope

Manufactured by Adobe

The LSM 780 is a high-performance confocal microscope designed for advanced imaging applications. It utilizes a laser scanning system to capture detailed, high-resolution images of samples. The core function of the LSM 780 is to provide researchers with a versatile tool for non-invasive, three-dimensional visualization and analysis of a wide range of specimens.

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Lab products found in correlation

A1 laser scanning confocal microscope, by Nikon (1 mentions) Nis elements software, by Nikon (1 mentions) Plan apo, by Nikon (1 mentions) Ti e microscope, by Nikon (1 mentions) Goat anti sox2, by Santa Cruz Biotechnology (1 mentions) Rabbit anti vimentin, by Cell Signaling Technology (1 mentions) Rabbit anti e cadherin, by Cell Signaling Technology (1 mentions) Mouse anti cdx2, by BioGenex (1 mentions) Rabbit anti pdgfrb, by Cell Signaling Technology (1 mentions) Rabbit anti cdx2, by Abcam (1 mentions) Fluorescent conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions) Rabbit anti lef1, by Cell Signaling Technology (1 mentions) Hoechst 33258, by Merck Group (1 mentions)

4 protocols using lsm 780 confocal microscope

Lysozyme Detection in Fly Brains

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A Zeiss LSM 780 confocal microscope was used for fluorescent imaging; the images were processed using LSM software or Adobe Photoshop. All images were treated identically. To detect lysozyme species by emission spectroscopy, samples were excited with a laser tuned to 488 nm and emission spectra were recorded using a tunable In Tune laser (488–640 nm). Spectra were collected from a minimum of five individual spots within a minimum of three different fly brains. Fluorescence life time imaging was carried out as described in [18 (link)], using 63x magnification.

Bergkvist L., Richards D.R., Bernardo-Gancedo A., Kumita J.R., Nilsson P.R, & Brorsson A.C. (2020). Serum amyloid P component promotes formation of distinct aggregated lysozyme morphologies and reduces toxicity in Drosophila flies expressing F57I lysozyme. PLoS ONE, 15(1), e0227227.

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Confocal Microscopy Image Analysis

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All images were taken with a Zeiss LSM780 confocal microscope and analyzed with Photoshop CS6 (Adobe). ZEN 2010 was applied for image acquisition and processing. Brightness or expression quantity was measured using ImageJ when needed.

Li Y, & Jiao J. (2020). Deficiency of TRPM2 leads to embryonic neurogenesis defects in hyperthermia. Science Advances, 6(1), eaay6350.

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In utero Electroporation Imaging Protocol

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For the in utero electroporation experiments, images were acquired in 1024 × 1024 mode with a Nikon Ti-E microscope equipped with the A1 laser scanning confocal microscope. We used the following objective lenses (Nikon): 10x PlanApo, NA 0.45 (for images of cortical slices), 60x Apo TIRF, NA 1.49 (analyses the leading process and morphometric analyses of multipolar and bipolar cells). Images were acquired using Nikon software NIS-Elements (Nikon Corporation, Melville, NY). The immunohistochemistry performed for Nav1 localization was acquired with Zeiss LSM780 confocal microscope, and digital images were adjusted for brightness and contrast in Photoshop (Adobe).

Powers R.M., Daza R., Koehler A.E., Courchet J., Calabrese B., Hevner R.F, & Halpain S. (2022). Growth cone macropinocytosis of neurotrophin receptor and neuritogenesis are regulated by neuron navigator 1. Molecular Biology of the Cell, 33(7), ar64.

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Multimarker Immunofluorescence Characterization

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We performed antibody staining of the differentiated cells according to standard protocols. The following primary antibodies were used: Rabbit anti-CDX2 (Abcam), Mouse anti-CDX2 (BioGenex), Goat anti-SOX2 (Santa Cruz), Rabbit anti-E-Cadherin (Cell signaling), Rabbit anti-Vimentin (cell signaling), Rabbit anti-FoxF1 (abcam), Rabbit anti-PDGFRB (cell signaling), Rabbit anti-LEF1 (cell signaling), Rabbit anti-Hand1 (Novus Biologicals) and Mouse anti-APLNR (R&D Systems). We stained the nuclei by using Hoechst 33258 (Sigma). The primary antibodies were detected by fluorescent-conjugated secondary antibodies from Jackson Immuno Research Laboratories. We collected the images by using the Zeiss LSM 780 confocal microscope and processed the images with Adobe Illustrator.

Zhang X., Chen Y., Ye Y., Wang J., Wang H., Yuan G., Lin Z., Wu Y., Zhang Y, & Lin X. (2016). Wnt signaling promotes hindgut fate commitment through regulating multi-lineage genes during hESC differentiation. Cellular signalling, 29, 12-22.

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