Horseradish peroxidase conjugated goat anti rabbit igg antibody

Tissue lysates were prepared as in previous studies [12 (link)]. The protein extracts from the renal cortex were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then transferred to polyvinylidene fluoride membranes. The membranes were blocked with 5% nonfat milk for 2 h at 37°C and then were incubated overnight at 4°C with anti-E-cadherin, α-SMA, monocyte chemotactic factor-1 (MCP-1), intercellular adhesion molecule (ICAM-1), phosphorylated signal transducer, and activator of transcription 1 (p-STAT1), p-STAT3, total STAT1, total STAT3, or β-actin antibodies. The anti-β-actin antibody was purchased from ZSGB-BIO (Beijing, China). The anti-p-STAT and total STAT antibodies were purchased from Cell Signaling (Boston, MA, USA). The other antibodies were purchased from Proteintech Group (Chicago, IL, USA). Thereafter, the membranes were incubated with a horseradish peroxidase-conjugated goat anti-rabbit IgG antibody (ZSGB-BIO) and imaged using the Odyssey Infrared Imaging System (LI-COR, Lincoln, NE, USA).