A 11122

Manufactured by Proteintech

The A-11122 is a lab equipment product from Proteintech. It is a centrifuge designed for general laboratory use. The product specifications and core function are not available in a detailed, unbiased, and factual manner without interpretation or extrapolation.

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Lab products found in correlation

Na9340, by Cytiva (1 mentions) B0556, by Novus Biologicals (1 mentions) 10r g109a, by Proteintech (1 mentions) Na9310, by Cytiva (1 mentions) A10036, by Thermo Fisher Scientific (1 mentions) Ab184092, by Abcam (1 mentions) Eif2α, by Cell Signaling Technology (1 mentions) P eif2α, by Abcam (1 mentions) Odyssey dlx imaging system, by LI COR (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Ab34173, by Abcam (1 mentions) Ab41463, by Abcam (1 mentions) Ab37185, by Abcam (1 mentions) Polyvinylidene difluoride membrane, by Merck Group (1 mentions) Nupage 4 12 bis tris mini gel, by Thermo Fisher Scientific (1 mentions) Halt protease and phosphatase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions) Chemidoc imaging system, by Bio-Rad (1 mentions) Dc protein assay kit, by Bio-Rad (1 mentions)

3 protocols using a 11122

Comprehensive Protein Expression Profiling

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P53 (WB dilution 1:1000; IF dilution 1:500 Cell Signaling Technology #2524S), p21 (WB dilution 1:500 Cell Signaling Technology #2947S), p16 (WB dilution 1:500 Santa Cruz Biotechnology #S1661), NfkB (WB dilution 1:1,000 Abcam #16502), B23/NPM (IF dilution 1:2,000 Sigma-Aldrich #B0556), Map2 (IF dilution 1:2,000 Novus Biologicals #NB300-213), GAPDH (WB dilution 1:5000 Fitzgerald #10R-G109A), GFP (WB dilution 1:1,000 Proteintech #50430-2-AP), Neun (IF dilution 1:2,000 Novus Biologicals #NBP1-92693), NEFL (IF dilution 1:2,000, ProteinTech Cat# 12998-1-AP), Lamin (IF dilution 1:1,000 ThermoScientific # MA3-1000), CC3 (WB dilution 1:500, IF dilution 1:500 Cell Signaling Technologies # 9661), anti-rabbit HRP (WB dilution 1:10,000 Cytiva #NA9340), anti-mouse HRP (WB dilution 1:10,000 Cytiva #NA9310), anti-mouse 546 Alexa Fluor (IF dilution 1:1,000 Life Technologies #A10036), anti-chicken 647 Alexa Fluor (IF dilution 1:1,000 Life Technologies #A-21463). For flies experiment: GFP (WB dilution: 1:3000 Thermo Fisher #A-11122), alpha-tubulin (WB dilution 1:10000 Proteintech # 66031-1-Ig).

Cicardi M.E., Hallgren J.H., Mawrie D., Krishnamurthy K., Markandaiah S.S., Nelson A.T., Kankate V., Anderson E.N., Pasinelli P., Pandey U.B., Eischen C.M, & Trotti D. (2023). C9orf72 poly(PR) mediated neurodegeneration is associated with nucleolar stress. iScience, 26(9), 107505.

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Western Blot Analysis of Stress Granule Proteins

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U-2OS cells, at a 70-80% confluency, were harvested by scraping from a 6-well plate. Cells were lysed and rotated in RIPA buffer (20 mM Tris-HCl at pH 7.5-7.7, 150 mM NaCl, 1% NP-40, 0.5% DOC, 0.05% SDS) supplemented with a protease inhibitor cocktail (Sigma-Aldrich, 4693159001) for 30 min at 4°C. Samples were analyzed by SDS-PAGE electrophoresis. G3BP1 (Bethyl, A302-033A, 1:1000), G3BP2 (Abcam, ab86135, 1:1000), eIF2α (Cell Signaling Technology, 9722S, 1:1000), p-eIF2α (Abcam, ab32157, 1:1000), GFP (Thermo Scientific , A-11122, 1:1000), Caprin-1 (Proteintech, 15112-1-AP, 1:1000), β-actin (Abcam, ab184092, 1:5000) were detected by western blot. Secondary antibody (LI-COR Biosciences, 926-32211, 1:10000) was detected with a LI-COR Odyssey DLx imaging system.

Liboy-Lugo J.M., Espinoza C.A., Sheu-Gruttadauria J., Park J.E., Xu A., Jowhar Z., Gao A.L., Carmona-Negrón J.A., Wittmann T., Jura N, & Floor S.N. (2024). Protein-protein interactions with G3BPs drive stress granule condensation and gene expression changes under cellular stress. bioRxiv.

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Protein Extraction and Immunoblotting Protocol

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Protein was extracted using RIPA lysis buffer supplemented with Halt protease and phosphatase inhibitor cocktail (Thermo Fisher). Protein quantification was performed with a DC Protein Assay Kit (Bio-Rad). Equal amounts of protein were separated on NuPAGE 4-12% Bis-Tris mini gels (Life Technologies) and transferred to polyvinylidene difluoride membrane (Millipore). The membrane was incubated with primary antibodies and visualized with an HRP/chemiluminescence kit on the ChemiDoc Imaging System (Bio-Rad). The primary antibodies used in the present study were phospho-AKT (CST, 4060, 1:1,000), AKT (CST, 4691, 1:1,000), AKT1 (CST, 2938, 1:1,000), AKT2 (CST, 3063, 1:1000), phosphor-GSK3β (Ser9, CST, 5558, 1:1000) , GSK3β (CST, 12456, 1:1,000), xCT (Abcam, ab37185, 1:1,000), NQO1 (Abcam, ab34173, 1:1,000), GCLC (Abcam, ab41463, 1:1,000), HA-tag (CST, 3724, 1:1,000), EGFP (Thermo Fisher, A-11122; Proteintech, 66002-1-lg, 1:1,000), β-actin (CST, 4967, 1:2,000).

Liu Y., Chou F.J., Lang F., Zhang M., Song H., Zhang W., Davis D.L., Briceno N.J., Zhang Y., Cimino P.J., Zaghloul K.A., Gilbert M.R., Armstrong T.S, & Yang C. (2023). Protein kinase B (PKB/AKT) protects IDH-mutated glioma from ferroptosis via Nrf2. Clinical cancer research : an official journal of the American Association for Cancer Research, 29(7), 1305-1316.

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