Dulbecco s modified eagle s minimal essential medium

The THP-1 human pro-monocytic cell line was grown in Dulbecco’s modified Eagle’s minimal essential medium (Sigma–Aldrich) supplemented with 10% fetal calf serum (Lonza, Verviers, Belgium) and 1% of an antibiotic/antimycotic (AB/AM) solution (Lonza) at 37 °C in a 5% CO₂ atmosphere.

HepG2 (hepatoma) cell line and HepG.2.215 (HBV-infected hepatoma cells) were generous gift from the lab of Prof. Shaul, Weizmann Institute of Science in Rehovot, Israel. These cells were maintained in Dulbecco’s modified Eagle’s minimal essential medium (Biological Industries, Israel), as previously described (Rechtman et al., 2010 (link)). Cells were grown to reach near confluence 24 h prior to transfection, which was carried out using the Lipofectamin 2000 reagent (Invitrogen Carlsbad, California USA), according to the manufacture’s instructions.
HepAD38 cells were generous gift from the lab of Prof. Seeger, Fox Chase Cancer Center, PA USA, and David Durantel Cancer Research Center of Lyon, France. These cells were cultured in a Dulbecco’s modified Eagle’s minimal essential medium with 10% FCS with or without 0.3 μg/mL tetracycline (Sigma St. Louis, MO, USA) for 7 days before analyzing the cells.
All cell lines were treated with increasing concentrations of Calcitriol (0–100 nM) (Sigma St. Louis, MO, USA) for 24 h.