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The BP399 is a single-channel electronic pipette from Thermo Fisher Scientific. It is designed for precise and accurate liquid handling in various laboratory applications. The BP399 features an ergonomic design, digital volume display, and adjustable volume range to suit a variety of laboratory tasks.

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4 protocols using bp399

1

Biotinylated DNA Transcription Templates

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5′ biotinylated or 5′ biotinylated and 3′ Dy-549-labelled DNA in vitro transcription templates (prepared according to methods and approaches Section 2.3).
Streptavidin-coated DNA capture surface (prepared according to methods and approaches Section 2.4).
PBS (Fisher Scientific; BP399).
Tween 20 (Fisher Scientific; BP337).
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2

Microarray Slide Preparation Protocol

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NEXTERION Slide H NHS-activated microarray slides (Schott; 1070936)
LifterSlips (Thermo Scientific; 22X25I-2-4816 and 24X60I-2-4733).
Streptavidin (Sigma; S0677-1MG).
Phosphate-buffered saline (PBS) (Fisher Scientific; BP399).
Tween 20 (Fisher Scientific; BP337).
Ethanolamine (Sigma; E9508).
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3

RNA Capture Surface Preparation

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DNA in vitro transcription template array (prepared according to methods and approaches Section 2.5).
Streptavidin-coated RNA capture surface (prepared according to methods and approaches Section 2.4).
Parafilm (Bemis Flexible Packaging).
MEGAscript T7 Transcription Kit (Invitrogen; AMB1334-5).
PBS (Fisher Scientific; BP399).
Tween 20 (Fisher Scientific; BP337).
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4

RNA Capture Array Transcription

Check if the same lab product or an alternative is used in the 5 most similar protocols
DNA in vitro transcription template array (prepared according to methods and approaches Section 2.5).
Streptavidin-coated RNA capture surface (prepared according to methods and approaches Section 2.4).
Parafilm (Bemis Flexible Packaging).
MEGAscript T7 Transcription Kit (Invitrogen; AMB1334-5).
Probe (see results and applications section for details).
PBS (Fisher Scientific; BP399).
Tween 20 (Fisher Scientific; BP337).
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