Transwell chambers with 8‐μm polycarbonate membrane filters (Corning, NY, USA) were used to make invasion analysis in A375 and A2058 cells. In short, the different treated A375 and A2058 cells (1 × 105) were harvested in 200 μL nonserum medium and then seeded into upper chambers. The lower chambers was supplemented with 500 μL medium with 10% FBS After incubating for 24 h, A375, and A2058 cells on the lower surface of the membrane were fixed with methanol, and 0.5% crystal violet (Sigma‐Aldrich) was used for invasive cells staining, and then Image J (NIH, Bethesda, Maryland, USA) software was used for invasive cells counting.
Transwell chambers with 8 μm polycarbonate membrane filters
Manufactured by Corning
Sourced in United States
Transwell chambers with 8‐μm polycarbonate membrane filters are a type of cell culture insert used for various cell-based assays. These chambers consist of a permeable polycarbonate membrane that allows for the study of cell migration, invasion, and co-culture experiments.
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2 protocols using transwell chambers with 8 μm polycarbonate membrane filters
1
Transwell Assay for Cell Invasion
2
Transwell Assay for Cell Migration and Invasion
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Cell migration and invasion were determined using transwell chambers with 8 μm polycarbonate membrane filters (Corning, Corning, NY, USA). The transfected Caki-1 and 786-O cells were injected into the upper chamber. Meanwhile, medium with 10% FBS was added as an attractant in the lower chamber. After 24 h of culture, the cells were fixed with methanol and stained with 0.1% crystal violet (Solarbio). The migrated cells were counted under a microscope at 100× magnification. For cell invasion test, the difference was that transwell chamber was pre-coated with Matrigel (Corning).
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