Chromatographic grade n-hexane and methanol were purchased from J&K Scientific Ltd (China). Sulfuric acid (purity 95–98 %) was analytical grade and was obtained from Tianjin Kemiou Chemical Reagent Co., Ltd (China). Chemically pure glyceryl trioleate (TAG) was purchased from Sinopharm Chemical Reagent Co., Ltd (SCRC) China. Methyl hexadecanoate was purchased from Sigma-Aldrich (USA). Soybean oil was purchased from a local supermarket, and the microalgal lipids were prepared using I. zhangjiangensis and Chaetoceros sp. cultured in our lab. Chlorella Vulgaris and Nannochloropsis were obtained from South China University of Technology and Beihang University, respectively.
Syringe pumps were purchased from Baoding Longer Precision Pump Co., Ltd. The Y-shaped MR is a simple triple valves with a 0.25 mm inner diameter from Dalian Elite Analytical Instruments Co., Ltd. The T-shaped and Q-shaped with 0.25 mm inner diameter MRs used in the studies were manufactured using glasses.
Syringe pumps were purchased from Baoding Longer Precision Pump Co., Ltd. The Y-shaped MR is a simple triple valves with a 0.25 mm inner diameter from Dalian Elite Analytical Instruments Co., Ltd. The T-shaped and Q-shaped with 0.25 mm inner diameter MRs used in the studies were manufactured using glasses.