Dulbecco’s Modified Eagle Medium—high glucose (DMEM-HG) (12800-58), Roswell Park Memorial Institute (RPMI)-1640, DMEM/Ham’s F-12 (GIBCO-Invitrogen, Carlsbad, CA, USA), fetal bovine serum (FBS), phosphate-buffered saline (PBS)and trypsin-EDTA solution were purchased from Gibco (Grand Island, NY, USA) Dimethyl sulfoxide (DMSO), and sulforhodamine B (SRB) was purchased from Sigma Chemical, Inc. (St Louis, MO, USA) The substrate of caspase-9 (LEHD-para-nitroaniline; LEHD-p-NA), caspase-8 (IETD-para-nitroaniline; IETD-p-NA), caspase-3 (DEVD-para-nitroaniline; DEVD-p-NA), and SuperSignal West Pico Chemiluminescent Substrate were obtained from Invitrogen (Thermo Fisher Scientific Inc., Waltham, MA, USA). Primary antibodies against caspase-9 (ab32539), caspase-8 (ab25901), caspase-7 (ab25900), BAX (ab32503), Bcl-xl (ab32370), Bid (ab2388), Noxa (ab13654), actin (ab8227) and peroxidase-labeled secondary antibodies; anti-rabbit IgG (ab97051), anti-mouse IgG (ab97046) were purchased from Abcam (Cambridge, UK). Protease inhibitor was obtained from Roche Diagnostics, Mannheim, Germany.
Devd p na
Manufactured by Thermo Fisher Scientific
Sourced in United States
DEVD-p-NA is a substrate used for the colorimetric detection of caspase-3/7 activity. It is a tetrapeptide sequence (Asp-Glu-Val-Asp) coupled to the chromophore p-nitroaniline (p-NA). Upon cleavage by caspase-3/7, the p-NA is released, resulting in a measurable color change that can be quantified spectrophotometrically.
Automatically generated - may contain errors
Lab products found in correlation
Ab32539, by Abcam (1 mentions)
Ab25901, by Abcam (1 mentions)
Ab13654, by Abcam (1 mentions)
Ab32503, by Abcam (1 mentions)
Lehd para nitroaniline lehd p na, by Thermo Fisher Scientific (1 mentions)
Ietd para nitroaniline ietd p na, by Thermo Fisher Scientific (1 mentions)
Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions)
Dmem ham s f12, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Trypsin edta solution, by Thermo Fisher Scientific (1 mentions)
Ab8227, by Abcam (1 mentions)
Protease inhibitor, by Roche (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Horse serum, by Thermo Fisher Scientific (1 mentions)
Penicillin g sodium, by Thermo Fisher Scientific (1 mentions)
Epidermal growth factor (egf), by Merck Group (1 mentions)
2 7 dichlorodihydrofluorescein diacetate dcfh da, by Merck Group (1 mentions)
3 3 dihexyloxacarbocyanine iodide dioc6, by Merck Group (1 mentions)
Annexin 5 fluos kit, by Roche (1 mentions)
2 protocols using devd p na
1
Apoptosis Pathway Analysis Protocol
2
Cell Culture Reagents and Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Dulbecco's modified Eagle medium (DMEM), Roswell Park Memorial Institute (RPMI) 1640 medium, Dulbecco's modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), fetal bovine serum (FBS), horse serum, streptomycin, and penicillin G sodium were obtained from Gibco BRL (Thermo Fisher Scientific Inc., Waltham, MA, USA). Hydrocortisone, insulin, epidermal growth factor (EGF), dimethyl sulfoxide (DMSO), 3-(4,5-dimethyl)-2,5-diphenyl tetrazolium bromide (MTT), propidium iodide (PI), 3,3#-dihexyloxacarbocyanine iodide (DiOC 6 ), and 2#,7#-dichlorodihydrofluorescein diacetate (DCFH-DA) were obtained from Sigma-Aldrich (St Louis, MO, USA). Annexin V-FLUOS Kit was obtained from Roche (Indianapolis, IN, USA). Dihydroethidium (DHE), Rhod-2 AM, Fluo-3 AM, and the substrates of caspase-9 (LEHDpara-nitroaniline, LEHD-p-NA), caspase-8 (IETD-p-NA), and caspase-3 (DEVD-p-NA) were obtained from Invitrogen (Thermo Fisher Scientific Inc.). Folin-Ciocalteu (F-C) reagent was obtained from Fluka (Milwaukee, WI, USA). Cholera Toxin, Vibrio cholerae Inaba 569B was obtained from United States Biological, Salem, MA, USA.
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