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2 protocols using mgcl2

1

Fabrication of Hydrogel Composites

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PVA (PVA-1799), acrylic acid (AA), 2-hydroxy-2-methylpropiophenone (I1173), ZrOCl2·8H2O, chitosan (deacetylation degree ≥ 95%, viscosity is 100–200 mPa·s), and ZnCl2 were purchased from Shanghai Macklin Biochemical Co., Ltd. Agarose (high gel strength) and gelatin (gel strength ~250 g Bloom) were purchased from Shanghai Aladdin Bio-Chem Technology Co., Ltd. AlCl3 was purchased from Sinopharm Chemical Reagent Co., Ltd. Gly, K2CO3, NaBr, KCl, CaCl2, BaCl2, MgCl2, Na2SO4, NaCl, Na3Cit, NaAc, Na2HPO4, and Na2CO3 were purchased from Guangzhou Chemical Reagent Factory. The deionized water was obtained using a water purification system.
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2

Keratinocyte serum-free medium protocol

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Keratinocyte serum-free medium (K-SFM), Hank’s balanced salt solution, trypsin, penicillin, and streptomycin were purchased from Gibco (Carlsbad, CA, USA). Fetal bovine serum was purchased from Hyclone Laboratories (Logan, UT, USA). ACh, L-NG-nitro-arginine (L-NNA), indomethacin, prostaglandin E2 (PGE2), U73122, and thapsigargin (Tg) were purchased from Sigma Aldrich (St. Louis, MO, USA). Fluo-3 AM was purchased from Molecular Probes (Eugene, OR, USA). Atropine sulfate was purchased from Hengjian Pharmacy (Jiangmen, China). NaCl, KCl, CaCl2, MgCl2, NaHCO3, MgSO4, KH2PO4, Glucose, and Na2HPO4 were purchased from Guangzhou Chemical Reagent Factory (Guangzhou, China). N-2-hydroethypiperazine-N’-2-ethanessulfonic acid (HEPES) was purchased from Biocolor BioScience & Technology (Shanghai, China).
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