Strains, plasmids and primers used in this study are listed in Tables S2-S3 respectively. nleF was amplified from EPEC E2348/69 and C. rodentium ICC169 genomic DNA by PCR. Site-directed mutagenesis was carried out by inverse PCR using KOD Hot Start polymerase and mismatch primers. All constructs were confirmed by sequencing (GATC biotech). For Western Blot, Mouse monoclonal anti-caspase-4 clone 4B9 (sc-56056; Santa Cruz), anti-α-Tubulin clone DM1A (T6199), mouse polyclonal antibody anti-caspase-11 p20 clone A-2 (sc-374615; Santa cruz) and anti-pro-IL-18 (CPTC-IL18-1; DSHB), the rabbit monoclonal anti-IL-18 (PM014; MBL), anti-caspase-5 (4429; Cell signalling) and the rabbit polyclonal antibody anti-GFP (Ab290; Abcam) were used as primary antibodies. Horse radish peroxidase (HRP)-conjugated goat anti-rabbit IgG (Fc fragement; catalog no.111-035-008; Jackson immunoresearch) and HRP-conjugated goat anti-mouse IgG (Fc fragement; catalog no, 115-035-008; Jackson immunoresearch) were used as secondary antibodies.
Anti caspase 11 p20 clone a 2
Manufactured by Santa Cruz Biotechnology
Anti-caspase-11 p20 clone A-2 is a laboratory reagent that can be used to detect the p20 subunit of caspase-11. Caspase-11 is an enzyme involved in the inflammatory response and programmed cell death. This product can be utilized in various research applications that require the identification or quantification of the caspase-11 p20 subunit.
Automatically generated - may contain errors
Lab products found in correlation
Anti gfp ab290, by Abcam (2 mentions)
Anti caspase 4 clone 4b9, by Santa Cruz Biotechnology (2 mentions)
Anti α tubulin clone dm1a t6199, by Santa Cruz Biotechnology (2 mentions)
Hrp conjugated goat anti rabbit igg fc fragement, by Jackson ImmunoResearch (2 mentions)
Hrp conjugated goat anti mouse igg fc fragement, by Jackson ImmunoResearch (2 mentions)
Sc 374615, by Santa Cruz Biotechnology (2 mentions)
Anti caspase 5, by Cell Signaling Technology (2 mentions)
2 protocols using anti caspase 11 p20 clone a 2
1
Molecular Cloning and Analysis
2
Molecular Cloning and Analysis
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Strains, plasmids and primers used in this study are listed in Tables S2-S3 respectively. nleF was amplified from EPEC E2348/69 and C. rodentium ICC169 genomic DNA by PCR. Site-directed mutagenesis was carried out by inverse PCR using KOD Hot Start polymerase and mismatch primers. All constructs were confirmed by sequencing (GATC biotech). For Western Blot, Mouse monoclonal anti-caspase-4 clone 4B9 (sc-56056; Santa Cruz), anti-α-Tubulin clone DM1A (T6199), mouse polyclonal antibody anti-caspase-11 p20 clone A-2 (sc-374615; Santa cruz) and anti-pro-IL-18 (CPTC-IL18-1; DSHB), the rabbit monoclonal anti-IL-18 (PM014; MBL), anti-caspase-5 (4429; Cell signalling) and the rabbit polyclonal antibody anti-GFP (Ab290; Abcam) were used as primary antibodies. Horse radish peroxidase (HRP)-conjugated goat anti-rabbit IgG (Fc fragement; catalog no.111-035-008; Jackson immunoresearch) and HRP-conjugated goat anti-mouse IgG (Fc fragement; catalog no, 115-035-008; Jackson immunoresearch) were used as secondary antibodies.
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