Lncap cells

LNCaP and PC3 human prostate cancer cells were obtained from American Type Culture Collection (ATCC, Manassas, VA) and were maintained in Roswell Park Memorial Institute medium (RPMI 1640) supplemented with 10% FBS (Wisent Bioproducts, St Bruno, Québec). PACE4 knockdown LNCaP were cultivated as described previously [21] (link). Cells were grown at 37°C in a water-saturated atmosphere with 5% CO₂. Cells were grown in complete media and harvested at their exponential growing state. Four- to 6-week-old male athymic nude mice (Nu/Nu; Charles River Laboratories, LaSalle, Québec) were inoculated subcutaneously on the shoulders with a suspension of 1 × 10⁶ PC3 cells in 100 μl of PBS or on the hips with 2.5 × 10⁶ LNCaP cells in 200 μl of ice-cold PBS/Matrigel (1:1; BD Biosciences, Bedford, MA). Mice were housed under pathogen-free conditions and the inoculations were done under isoflurane anesthesia in a sterile laminar flow hood. Tumors were given a minimum of 4 weeks to grow to a minimal diameter of 5 mm before being used for biodistribution or PET imaging studies. All animal studies were conducted in compliance with the Canadian Council on Animal Care guidelines and with the approval of the Animal Care Committee of the Université de Sherbrooke.