Fetal bovine serum (fbs)

Human bladder cancer cell lines (J82 and T24) were obtained from
Dr. P Guo (Xi’an Jiaotong University). The human lung cancer
cell line (A549) was purchased from ATCC. The human ovarian cancer
cell line (OVCAR3) was purchased from China center for type culture
collection. The human ovarian cancer cell line (SKOV3) was a gift
from Xiangya hospital, China. The human umbilical vein endothelial
cell line (HUVEC) also was a gift from Prof. F. Y. Chen (Shanghai
Jiaotong University, China). SKOV3 and OVCAR3 cells were grown in
RPMI 1640 (Procell, China) supplemented with 20% fetal bovine serum
(Procell, China) and 1% penicillin/streptomycin mixture. A549 and
HUVEC cells were grown in DMEM (Hyclone, Logan, UT, USA) supplemented
with 10% fetal bovine serum (Procell, China) and 1% penicillin/streptomycin
mixture. J82 cells were grown in MEM (Procell, China) supplemented
with 10% fetal bovine serum (Procell, China) and 1% penicillin/streptomycin
mixture. T24 cells was grown in 5A (Procell, China) supplemented with
10% fetal bovine serum (Procell, China) and 1% penicillin/streptomycin
mixture. In addition, all cells were maintained in a 37 °C, 5%
CO₂ humidified incubator.

The cell lines (MDA-MB-231, MDA-MB-468, MDA-MB-436, MCF-10A, MCF-7, BT-474, and SK-BR3) were acquired from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and Procell (Wuhan, China). All cell lines except for MDA-MB-468 were cultured in a humidified incubator at 37°C with 5% CO2. MDA-MB-231 and MDA-MB-436 cells were grown in DMEM supplemented with 10% FBS (Procell, Wuhan, China) and 1% penicillin/streptomycin (Yeasen, Shanghai, China). MCF-10A, BT-474, and SK-BR3 cells were grown in appropriate special complete medium. MCF-7 cells were cultured in 1640 medium enriched with 10% FBS (Procell) and 1% penicillin/streptomycin (Yeasen). MDA-MB-468 cells were cultured in L-15 medium with 10% FBS (Procell) and 1% penicillin/streptomycin (Yeasen) in a humidified incubator at 37°C without CO2.