Log In Sign up
The largest database of trusted experimental protocols

Pen strep

Manufactured by Corning
Visit Supplier
Sourced in United States

Pen/Strep is a sterile solution containing the antibiotics penicillin and streptomycin. It is commonly used in cell culture applications to prevent bacterial contamination.

Automatically generated - may contain errors

Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Corning (33 mentions) Fetal bovine serum (fbs), by Merck Group (19 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (17 mentions) L glutamine, by Corning (16 mentions) Pen strep, by Thermo Fisher Scientific (16 mentions) Rpmi 1640, by Corning (14 mentions) Fetal bovine serum (fbs), by Corning (12 mentions) Sodium pyruvate, by Corning (11 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (10 mentions) Pen strep, by Merck Group (7 mentions) Mda mb 231, by American Type Culture Collection (6 mentions) L glutamine, by Thermo Fisher Scientific (6 mentions) Non essential amino acids (neaa), by Corning (6 mentions) Glutamax, by Thermo Fisher Scientific (5 mentions) Glutamine, by Corning (5 mentions) Puromycin, by Corning (5 mentions) Hygromycin b, by Corning (5 mentions) Dmem medium, by Corning (4 mentions) Mda mb 468, by American Type Culture Collection (4 mentions) Rpmi 1640, by Thermo Fisher Scientific (4 mentions)

129 protocols using pen strep

1

Cell Culture Protocols for Breast Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
MDA-MB-231, Hs578t, MCF-7, and MDA-MB-468 cells were purchased from American Type Culture Collection (ATCC). MDA-MB-231 and MCF-7 were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Corning CellGro., Manassas, VA, USA) supplemented with 10% fetal bovine serum (Sigma-Aldrich, St. Louis, MO, USA), 1% pen–strep, and 1% L-glutamine (Corning, CellGro). MDA-MB-468 was cultured in DMEM (Corning CellGro) supplemented with 10% fetal bovine serum, 1% pen–strep, 1% L-glutamine, 1% sodium pyruvate, and 1% Minimum Essential Medium (MEM) amino acids (Corning CellGro). Hs578t was cultured in MEM supplemented with 10% fetal bovine serum, 1% pen–strep, and 1% L-glutamine (Corning CellGro). SUM159PT cells were obtained from Asterand and cultured in Ham’s/F-12 (Corning CellGro) supplemented with 10% fetal bovine serum, 1% pen–strep, 1% L-glutamine, 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 1 μg/mL hydrocortisone, and 5 μg/mL insulin. All cell lines were maintained in a humidified incubator at 5% CO2 and 37 °C.
Andrade D., Mehta M., Griffith J., Oh S., Corbin J., Babu A., De S., Chen A., Zhao Y.D., Husain S., Roy S., Xu L., Aube J., Janknecht R., Gorospe M., Herman T., Ramesh R, & Munshi A. (2019). HuR Reduces Radiation-Induced DNA Damage by Enhancing Expression of ARID1A. Cancers, 11(12), 2014.
+ Open protocol
+ Expand
2

Isolation and Culture of Immune Cells for In Vitro Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
THP-1 cells, a human leukemia monocyte cell line, were maintained in RPMI-1640 Medium (Sigma-Aldrich) with 10% fetal bovine serum (FBS, Sigma-Aldrich), 1% L-glutamine (Corning), 2% of 5000 IU/mL of Penicillin and 5,000 μg/ml of Streptomycin Solution (Pen-Strep, Corning), 1% of 1 M HEPES (Corning), and 5 mM 2-Mercaptoethanol (Gibco). Cells were incubated at 37 °C with 5% CO2.
Primary neutrophils for antibody-dependent neutrophil phagocytosis (ADNP) assays were isolated from whole blood from healthy donors using Ammonium-Chloride-Potassium (ACK) Lysing Buffer (Quality Biological) followed by centrifugation and multiple wash steps prior to assay usage.
Primary natural killer cells for the antibody-dependent natural killer activation (ADNKA) assays were isolated from buffy coats from healthy donors using the RosetteSep NK cell enrichment kit (STEMCELL Technologies). Cells were incubated overnight at 37 °C with 5% CO2 in RPMI-1640 Medium (Sigma-Aldrich) with 10% FBS (Sigma-Aldrich), 1% L-glutamine (Corning), 2% of Pen-Strep (Corning), and 1% of 1 M HEPES (Corning) prior to assay usage.
Human embryonic kidney (HEK) 293 T cells expressing ACE2 for neutralization assays were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, Corning) with 10% FBS (VWR) and 1% Pen-Strep (Corning) and incubated in 37 °C with 5% CO2.
Bartsch Y.C., Chen J.W., Kang J., Burns M.D., St Denis K.J., Sheehan M.L., Davis J.P., Edlow A.G., Balazs A.B., Yonker L.M, & Alter G. (2022). BNT162b2 induces robust cross-variant SARS-CoV-2 immunity in children. NPJ Vaccines, 7, 158.
+ Open protocol
+ Expand
3

Stable Expression of Human VMAT2 in HEK293 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human embryonic kidney cells (HEK293, ATCC) were cultured at 37°C with 5% CO2 in media comprised of Dulbecco’s Modified Eagle Medium (DMEM, Corning), 10% Fetal Bovine Serum (FBS, Atlanta Biologicals), and 1% Penicillin-Streptomycin (Pen Strep, Corning). All human VMAT2-containing constructs were made in pcDNA3.1 vectors (Life Technologies) containing a zeocin resistance gene. Plasmids were transfected into HEK293 cells with Lipofectamine 2000 using the manufacturer protocol and stable cell lines were generated by repetitive rounds of limiting dilutions in selection media. HEK293 cells stably expressing human VMAT2 (HEK+VMAT2) or mCherry-tagged human VMAT2 (HEK+mCherry-VMAT2) were cultured at 37°C with 5% CO2 in selection media comprised of DMEM (Corning), 10% FBS (Atlanta Biologicals), 1% Pen Strep (Corning), and zeocin (100 μg/mL, InvivoGen). Experimental media used to optimize the 96-well plate screening assay and to screen pharmacological inhibitors of VMAT2 and environmental toxicants was comprised of DMEM without phenol red (Corning), 1% Pen Strep (Corning), and 1% L-glutamine (Gibco).
Black C.A., Bucher M.L., Bradner J.M., Jonas L., Igarza K, & Miller G.W. (2020). Assessing vesicular monoamine transport and toxicity using fluorescent false neurotransmitters. Chemical research in toxicology.
+ Open protocol
+ Expand
4

Culturing Primary Human Keratinocytes and THP-1 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human primary keratinocytes (HEKn, Gibco, C0015C) were cultured in DermaLife basal medium (Lifeline Cell Technology) supplemented with growth factors (DermaLife K LifeFactors kit, Lifeline Cell Technology), 1% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (Pen/Strep, Corning) and used up to passage 8. THP-1 cells (ATCC, TIB-202) were cultured in RPMI 1640 medium containing l-glutamine (Corning) and supplemented with 10% FBS and 1% Pen/Strep and differentiated with phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich) at a final concentration of 1 μM for 48 h. The HEKn and THP-1 cells were authenticated by the manufacturers. Murine BMDMs were cultured in DMEM (Corning) supplemented with 1% FBS with or without Pen/Strep. All cell lines were routinely tested for mycoplasma contamination.
Cai T., Reilly T.R., Cerio M, & Schmitt M.E. (1999). Mutagenesis of SNM1, Which Encodes a Protein Component of the Yeast RNase MRP, Reveals a Role for This Ribonucleoprotein Endoribonuclease in Plasmid Segregation. Molecular and Cellular Biology, 19(11), 7857-7869.
+ Open protocol
+ Expand
5

Murine Hepatocyte Cell Line Maintenance

Check if the same lab product or an alternative is used in the 5 most similar protocols
Hepa1-6 and AML-12 murine hepatocyte cell lines were obtained from the ATCC and maintained in DMEM, 4.5g/L glucose without sodium pyruvate (Thermo Scientific) and supplemented with Pen/Strep and Glutagro (Corning) and DMEM-F12 1:1, (ThermoFisher Scientific) supplemented with Pen/Strep, Glutagro (Corning), insulin-transferrin-selenium (ThermoFisher Scientific) and dexamethasone (100nM, Sigma Aldrich), respectively.
Byles V., Cormerais Y., Kalafut K., Barrera V., Hallett J.E.H., Sui S.H., Asara J.M., Adams C.M., Hoxhaj G., Ben‐Sahra I., & Manning B.D. (2021). Hepatic mTORC1 signaling activates ATF4 as part of its metabolic response to feeding and insulin.
+ Open protocol
+ Expand
6

Culturing Cancer Cell Lines for Hypoxia Studies

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Human acute T cell leukemia cell line Jurkat (American Type Culture Collection (ATCC), TIB-152), lung cancer cell line NCI-H292 (ATCC, CRL-1848) and ovarian cancer cell line SKOV3 (ATCC, HTB-77) was cultured in RPMI 1640 (Corning) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% penicillin-streptomycin (Pen/Strep, Corning). Embryonic kidney cell line HEK293T (ATCC, CRL-3216), lung cancer cell line A549 (ATCC, CCL-185) and epithelioid cervix carcinoma cell line Hela (ATCC, CBP-60232) were cultured in Dulbecco’ s modified Eagle’s medium (Corning) supplemented with 10% FBS and 1% Pen/Strep. Tumor cells carrying the report gene firefly luciferase (SKOV3-Luc, NCI-H292-Luc and A549-Luc cells) were engineered as previously described.36 (link) The cells were maintained in a humidified atmosphere containing 5% CO2 at 37°C. The hypoxia treatment (1% O2, 5% CO2 and 94 % N2) was carried out in Smartor 118 mobile three-gas control system (China Innovation Instrument) or by adding different concentration of CoCl2 into the culture medium to simulated chemical hypoxia.
The recombinant type 5 adenovirus expressing tumor antigen Her2 with reporter firefly luciferase (Ad5-Her2) was contracted for production to Shanghai Genechem Co., Ltd.
He H., Liao Q., Zhao C., Zhu C., Feng M., Liu Z., Jiang L., Zhang L., Ding X., Yuan M., Zhang X, & Xu J. (2021). Conditioned CAR-T cells by hypoxia-inducible transcription amplification (HiTA) system significantly enhances systemic safety and retains antitumor efficacy. Journal for Immunotherapy of Cancer, 9(10), e002755.
+ Open protocol
+ Expand
7

Murine Hepatocyte Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols
Hepa1-6 and AML-12 murine hepatocyte cell lines were obtained from the ATCC and maintained in DMEM, 4.5 g/L glucose without sodium pyruvate (Thermo Scientific) and supplemented with Pen/Strep and Glutagro (Corning) and DMEM-F12 1:1, (ThermoFisher Scientific) supplemented with Pen/Strep, Glutagro (Corning), insulin-transferrin-selenium (ThermoFisher Scientific) and dexamethasone (100 nM, Sigma Aldrich), respectively.
Byles V., Cormerais Y., Kalafut K., Barrera V., Hughes Hallett J.E., Sui S.H., Asara J.M., Adams C.M., Hoxhaj G., Ben-Sahra I, & Manning B.D. (2021). Hepatic mTORC1 signaling activates ATF4 as part of its metabolic response to feeding and insulin. Molecular Metabolism, 53, 101309.
+ Open protocol
+ Expand
8

Isolation and Culture of Primary Glial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
PIGPC were isolated as described previously (7) . PIGPC and U251 (Sigma; RRID:CVCL_0021) cells were cultured in DMEM (Corning) supplemented with 10% FBS (Biological Industries) and 1% PenStrep (Corning). HBVP (ScienCell) were cultured in PM (ScienCell) on 2 mg/cm 2 poly-l-lysine mol wt 70,000 to 150,000 (Sigma) coated plastic and used below passage 4. HMC3 (ATCC, CRL-3304, RRID:CVCL_II76) were cultured in DMEM (Corning) supplemented with 10% FBS (Biological Industries) and 1% PenStrep (Corning). BEND3 (ATCC, CRL-2299, RRID:CVCL_0170) were cultured in Endothelial Cell Growth Medium MV2 (PromoCell) on 0.1% gelatin coated plastic. Primary human astrocytes (3H Biomedical) were cultured in Astrocyte Medium (3H Biomedical) and used below passage 15. U3082 glioma cells were obtained from HGCC (hgcc.se, RRID:CVCL_IR93) and were cultured in HGC medium on laminincoated plates as described previously (18) . Cells were acquired 2015 to 2020, tested quarterly for mycoplasma (Eurofins, MycoplasmaCheck), and not further authenticated.
Berg T.J., Marques C., Pantazopoulou V., Johansson E., von Stedingk K., Lindgren D., Jeannot P., Pietras E.J., Bergström T., Swartling F.J., Governa V., Bengzon J., Belting M., Axelson H., Squatrito M, & Pietras A. (2021). The Irradiated Brain Microenvironment Supports Glioma Stemness and Survival via Astrocyte-Derived Transglutaminase 2. Cancer research, 81(8).
+ Open protocol
+ Expand
9

Maintenance of Ae. aegypti Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ae. aegypti A20 and Aag2 cells were maintained in Leibovitz’s L-15 media (Gibco) supplemented with 10% FBS (Atlanta Biologicals) and 1% Pen-strep (Corning) at 28 °C. Aag2 cells were also maintained in Schneider’s Drosophila Media (Thermo Fisher) with 10% FBS (Atlanta Biologicals) and 1% Pen-strep (Corning) at 28 °C. Both cell lines are available upon request.
Eggleston H, & Adelman Z.N. (2020). Transcriptomic analyses of Aedes aegypti cultured cells and ex vivo midguts in response to an excess or deficiency of heme: a quest for transcriptionally-regulated heme transporters. BMC Genomics, 21, 604.
+ Open protocol
+ Expand
10

Cell Culture Conditions for Diverse Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
iSLK.219 cells (a kind gift from Dr. Don Ganem) were maintained in DMEM medium (Corning) containing 10% tetracycline (Tet)-free FBS (Sigma), 1% Pen-Strep (Corning), 10 μg/ml puromycin (Corning), 250 μg/ml Geneticin (Corning), and 400 μg/ml hygromycin B (Corning). BCBL1-TREx-RTA cells (a kind gift from Dr. Jae Jung) were cultured in RPMI 1640 (Corning) medium supplemented with 10% tetracycline (Tet)-free FBS, 1% Pen-Strep, 1% L-glutamine, 1% sodium bicarbonate, 0.05 mM β-mercaptoethanol, and 20 μg/ml hygromycin. 293FT (Thermo Fisher, R7007) were maintained in DMEM medium containing 10% FBS (Millipore) and 1% Pen-Strep. All cells were maintained at 37°C in a 5% CO2 laboratory incubator subject to routine cleaning and decontamination.
Zhang H., Ni G, & Damania B. (2020). ADAR1 Facilitates KSHV Lytic Reactivation by Modulating the RLR-Dependent Signaling Pathway. Cell reports, 31(4), 107564.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!