FITC-labeled anti-mouse CD3 (145-2C11, #11-0031-82, 1:100) and GL7 (GL7, #13-5902-81, 1:200) were purchased from eBiosciences (San Diego, CA). Rat anti-mouse B220 (RA3-6B2, #550286, 1:100) and rat anti-mouse IgD (11-26c.2a, #553438, 1:100) were purchased from BD Biosciences (San Jose, USA). Biotinylated PNA (#B-1075,1:300), goat anti-mouse IgM (#FI-2020,1:100) and AMCA-labeled streptavidin (#SA-5008,1:300), biotinylated ulex europaeus agglutinin-1 (UEA-1, #B1065, 1:200) and FITC-labeled streptavidin (#SA-5001, 1:100) were purchased from Vector Laboratories (Burlingame, USA). Texas red-labeled goat anti-rat IgG (#T-6392, 1:500), Alexa Fluor® 488-labeled goat anti-rat IgG (#A-11006, 1:500), and Alexa Fluor® 488-labeled goat anti-mouse IgG (#A11001, 1:200) were purchased from Thermo Fisher Scientific (Waltham, USA). Rat anti-mouse cytokeratin 8 (#Troma-1 1:100) was purchased from Developmental Studies Hybridoma Bank (Iowa City, USA).
Fitc labeled streptavidin
Manufactured by Vector Laboratories
Sourced in United States
FITC-labeled streptavidin is a protein complex that consists of the bacterial protein streptavidin conjugated to the fluorescent dye fluorescein isothiocyanate (FITC). Streptavidin has a high affinity for the vitamin biotin, and the FITC label allows for the visualization of streptavidin-biotin interactions.
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Lab products found in correlation
Alexa fluor 488 labeled goat anti mouse igg, by Thermo Fisher Scientific (1 mentions)
Goat anti mouse igm, by Vector Laboratories (1 mentions)
Biotinylated ulex europaeus agglutinin 1 uea 1, by Vector Laboratories (1 mentions)
Rat anti mouse b220 ra3 6b2, by BD (1 mentions)
Biotinylated pna, by Vector Laboratories (1 mentions)
Facsverse flow cytometer, by BD (1 mentions)
Non enzymatic cell dissociation solution, by Merck Group (1 mentions)
Fitc labeled pna, by Merck Group (1 mentions)
2 protocols using fitc labeled streptavidin
1
Multicolor Flow Cytometry Staining
2
Flow Cytometric Characterization of Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were washed once with PBS and subsequently incubated with 500 μl of nonenzymatic cell dissociation solution (Sigma) until they detached (20 min). Cells were collected in 4 ml PBS and washed twice with 1 ml of 0.1% fetal calf serum in PBS centrifuging at 500g for 5 min in between. Cells were then incubated with IIH6C4 antibody (1:200 dilution; Merck Millipore) for 40 min, with biotin-labeled MAL II (10 μg/ml; Vector Laboratories) for 40 min or with FITC–labeled PNA (10 μg/ml; Sigma–Aldrich) for 90 min. Subsequently, cells were washed twice as described previously and incubated for 20 min with 1:200 diluted goat antimouse IgM-CFL (Santa Cruz) for the cells incubated with IIH6C4 antibody or with 5 μg/ml of FITC-labeled streptavidin (Vector Laboratories) for the cells incubated with MAL II. All cells were washed twice as described previously, resuspended in 400 μl PBS, and analyzed using a FACSverse flow cytometer (BD Biosciences).
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