Colorview 3 camera

Manufactured by Olympus

Sourced in Japan

The Colorview III camera is a high-performance digital camera designed for laboratory applications. It features a 5-megapixel CMOS sensor that captures detailed, color-accurate images. The camera is capable of delivering a resolution of up to 2592 x 1944 pixels and supports various image formats, including JPEG and TIFF. The Colorview III is equipped with a C-mount lens interface, allowing for the use of a wide range of compatible lenses.

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Lab products found in correlation

Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (2 mentions) Eclipse e600 microscope, by Olympus (2 mentions) Goat anti mouse igg conjugated to alexa 594, by Thermo Fisher Scientific (2 mentions) Cryostar nx70 cryostat, by Thermo Fisher Scientific (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Szx16 stereomicroscope, by Olympus (1 mentions) Microscopy slides, by Thermo Fisher Scientific (1 mentions) 1 4 diazabicyclo 2.2.2 octane dabco, by Merck Group (1 mentions) Bx60 microscope, by Olympus (1 mentions) Ix50 microscope, by Olympus (1 mentions) Bx50 fluorescent microscope, by Olympus (1 mentions) Bx50f4 microscope, by Olympus (1 mentions) Bx51 system microscope, by Olympus (1 mentions) Ax70 microscope, by Olympus (1 mentions) Bx50 compound microscope, by Olympus (1 mentions) Uplanfln objective, by Olympus (1 mentions) Ab11024, by Abcam (1 mentions) Ab9498, by Abcam (1 mentions) Prolong gold antifade, by Thermo Fisher Scientific (1 mentions) Ab7817, by Abcam (1 mentions)

Close spelling variants of this product

Colorview camera (8 citations) ColorView (6 citations) ColorView III and II cameras (2 citations)

15 protocols using colorview 3 camera

Sampling and Analyzing Aldrovanda Trap Content

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Aldrovanda plants were taken from the habitats and transferred into petri dishes filled with water. We inspected the plants for closed traps with dark biomass inside. We sampled only traps from the whorls 3–8 counted from the apex at the German site and whorl 2–12 at the Czech sites, to guarantee that compared traps were more or less of the same size. Filled snap-traps were dissected with small forceps and transferred into small glass vials or Eppendorf tubes filled with 70% ethanol at the German site and with 4% formaline at the Czech sites. All traps were dissected from various plants and various whorls to reduce sampling bias. After sampling, the plants were transferred back to the habitats.
Traps were opened using dissection needles and their content was analyzed and recorded with an Olympus SZX 16 stereo microscope and a colorview III camera (Olympus, Tokio, Japan). Species identification was performed according to Scourfield and Harding (1994) , Streble and Krauter (2006) , and Schaefer (2009) .

Horstmann M., Heier L., Kruppert S., Weiss L.C., Tollrian R., Adamec L., Westermeier A., Speck T, & Poppinga S. (2019). Comparative Prey Spectra Analyses on the Endangered Aquatic Carnivorous Waterwheel Plant (Aldrovanda vesiculosa, Droseraceae) at Several Naturalized Microsites in the Czech Republic and Germany. Integrative Organismal Biology, 1(1), oby012.

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Confocal Microscopy Imaging of Nuclei-Stained Cells

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Before imaging the cells with the confocal microscope, we stained the nuclei of the cells with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen/Molecular Probes, ref. D3571) diluted 1:40,000 in 1x PBS for 5 min at RT, mounted on microscopy slides (Thermo Scientific) with in-house-made Mowiol-Dabco (33.3% (v/v) glycerol containing 16.6% (w/v) Mowiol (Calbiochem, St. Louis, MO, USA) ref. 475904) and 2.5% (w/v) 1,4-diazabicyclo [2.2.2]octane (DABCO) (Sigma Aldrich, St. Louis, MO, USA) ref. D2, 780-2), and stored in the dark at 4 °C overnight.
The cells were imaged using an Olympus microscope IX81 with FluoView-1000 confocal setup, with the Olympus UPlanFLN 40x oil immersion objective (numerical aperture 1.30) and Olympus Immoil-F30CC immersion oil. The lasers used were a 405 nm multiline diode laser, a 488 nm argon laser, and 543 nm and 633 nm HeNe lasers. The following software settings were used in image acquisition: unidirectional scan mode, dwell time—4.0 µs/pixel, image size—640 × 640 pixels, aspect ratio—1:1, sequential line capture, Kalman averaging with 3 scans.
ISH-stained paraffin sections were imaged using an Olympus BX60 microscope fitted with an Olympus Colorview III camera.

Salmikangas S., Laiho J.E., Kalander K., Laajala M., Honkimaa A., Shanina I., Oikarinen S., Horwitz M.S., Hyöty H, & Marjomäki V. (2020). Detection of Viral −RNA and +RNA Strands in Enterovirus-Infected Cells and Tissues. Microorganisms, 8(12), 1928.

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Cell Surface Area Quantification

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Phase contrast images of adherent cells were taken with an Olympus IX50 microscope and an Olympus ColorView III camera (Olympus, Hamburg, Germany). The cell surface was calculated with the region of interest (ROI) manager tool of Fiji (Fiji is just ImageJ, http://fiji.sc/Fiji, [19 (link)]). For all cell lines the size of 10 cells from different passages was calculated and averaged.

Nemes A., Fortmann T., Poeschke S., Greve B., Prevedello D., Santacroce A., Stummer W., Senner V, & Ewelt C. (2016). 5-ALA Fluorescence in Native Pituitary Adenoma Cell Lines: Resection Control and Basis for Photodynamic Therapy (PDT)?. PLoS ONE, 11(9), e0161364.

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Microscopic Analysis of E. festucae Endophyte

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E. festucae Fl1 wild-type and acyA deletion mutants were grown in triplicate on potato dextrose broth (PDB, Difco, Le Pont, De Claix, France), either 1X or diluted 1:100 in water, containing 0.8% (w/v) agarose. The broths were inoculated and grown for 5 days at 22°C in continuous light. Alternatively, cultures were grown on the same medium on sterile microscope slides and incubated as described above. If grown in a culture dish, 0.5 cm² of agar was cut from the outer edge of the colony, mounted onto a microscope slide, and a drop of water and a cover glass placed directly onto the specimen. If grown on a microscope slide, a drop of water was added to the mycelium and a cover glass applied. Cultures were imaged by bright field microscopy using a BX50 fluorescent microscope (Olympus, Tokyo, Japan) and a 40X UPLANFLN objective with a 0.75 numerical aperture. Images were taken using an Olympus Colorview III camera with AnalySIS^B image processing software. Endophytes in the epidermal layer of the host leaf sheath were stained with 0.15% (w/v) aniline blue and examined by bright field microscopy as described previously (Christensen et al., 2008 (link)). Leaf sheaths from two tillers per plant, and at least three infected plants per strain in each inoculation experiment, were examined.

Voisey C.R., Christensen M.T., Johnson L.J., Forester N.T., Gagic M., Bryan G.T., Simpson W.R., Fleetwood D.J., Card S.D., Koolaard J.P., Maclean P.H, & Johnson R.D. (2016). cAMP Signaling Regulates Synchronised Growth of Symbiotic Epichloë Fungi with the Host Grass Lolium perenne. Frontiers in Plant Science, 7, 1546.

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Microscopic Bead Size Analysis

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Optical microscopy imaging was performed using an Olympus BX50F4 microscope equipped with phase contrast filters. Images were captured with a ColorView III camera (Olympus, Japan) which is a high resolution digital camera and was attached to the microscope. Bead sizing was performed manually using the sizing tool of AnalySIS software (Soft Imaging System GmbH).

Heaysman C.L., Phillips G.J., Lloyd A.W, & Lewis A.L. (2016). Synthesis and characterisation of cationic quaternary ammonium-modified polyvinyl alcohol hydrogel beads as a drug delivery embolisation system. Journal of Materials Science. Materials in Medicine, 27, 53.

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Microscopic Analysis of Giant Cells in Root Galls

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Microscopic examination of the giant cells was performed as described by Ji et al. [50 (link)]. Root galls were collected at 7 dpi, fixed in 1x PIPES buffer with 2 % glutaraldehyde overnight, and then dehydrated in a series of ethanol dilutions and infiltrated in Technovit 7100. The infiltrated roots were embedded in plastic cubes filled with Technovit 7100 plus Hardener II as described by the manufacturer. The embedded gall tissues were sectioned into 10-μm slices with a Leica RM2265 motorized rotary microtome (Leica Microsystems, Nussloch, Germany). Sections of the galls were maintained on cover glass and stained in 0.05 % toluidine blue for 5 min. Microscopic observations were performed using a BX 51 system microscope (Olympus Optical Company, Tokyo, Japan) at a 40x magnification, and images were obtained with an Olympus ColorView III camera. The experiment was repeated twice, and 10 galls from each treatment were observed.

Huang W.K., Ji H.L., Gheysen G., Debode J, & Kyndt T. (2015). Biochar-amended potting medium reduces the susceptibility of rice to root-knot nematode infections. BMC Plant Biology, 15, 267.

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Histological Analysis of Murine Tissues

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The remaining half of each murine LV and TA tissues were embedded in OCT (Optimal Cutting Temperature) compound. Von Kossa staining to determine calcium deposits and Sirius red staining to determine type I and III collagen fibers were undertaken on 5μm-thick frozen sections. Tissue sections were analyzed under an Olympus AX70 microscope (Olympus, Tokyo, Japan). Quantification of red-stained fibrotic areas was obtained from five different fields for each tissue sample at 400x magnification (corresponding to a field size of 0.344-mm²), using Cell-F software and a ColorView III camera (Olympus, Tokyo, Japan). Results were expressed as the mean value of the five different field findings from each tissue sample. Aorta thickness was measured in 5 μm-thick frozen sections using Image J software (National Institutes of health NIH, USA) (three to six aorta sections per animal).

Omarjee L., Roy C., Leboeuf C., Favre J., Henrion D., Mahe G., Leftheriotis G., Martin L., Janin A, & Kauffenstein G. (2019). Evidence of Cardiovascular Calcification and Fibrosis in Pseudoxanthoma Elasticum Mouse Models Subjected to DOCA-Salt Hypertension. Scientific Reports, 9, 16327.

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Hoechst 33342 Staining of Live Neurons

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Control and OGD/R-subjected live neurons were incubated on DIV 10 and DIV 11 respectively, with 8 μM Hoechst 33342 (Thermo Scientific) in culture medium. After 30 min, the staining solution was replaced with HBSS (Gibco) and images were taken using Olympus IX71 fluorescence microscope equipped with Olympus Colorview III camera (Olympus, Tokyo, Japan) and dedicated Cell^F software. Nucleus counting was performed using the Cell Counter plugin for ImageJ (NIH, Bethesda, MD, USA).

Wojtyniak P., Boratynska-Jasinska A., Serwach K., Gruszczynska-Biegala J., Zablocka B., Jaworski J, & Kawalec M. (2022). Mitofusin 2 Integrates Mitochondrial Network Remodelling, Mitophagy and Renewal of Respiratory Chain Proteins in Neurons after Oxygen and Glucose Deprivation. Molecular Neurobiology, 59(10), 6502-6518.

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NBT Staining for Superoxide Detection in E. festucae

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Superoxide radicals in E. festucae in axenic culture were stained using NBT (Sigma-Aldrich) as described by Tanaka et al. (2006 (link)). Three replicates for each strain were included in the analysis and the experiment was repeated twice. Strains were grown on PDA for 1 week at 22°C under 8 h light and 16 h dark, and mycelia stained for 5 h at 22°C in continuous light by incubation in 20 μL of 0.05% (w/v) NBT dissolved in 0.05 M sodium phosphate pH 7.5. The reaction was stopped by removing the stain and adding 40 μL of absolute ethanol. The ethanol was removed after 5 min, and mycelia were analyzed at 400X magnification under bright field illumination using an Olympus BX50 compound microscope with a 40X UPLANFLN objective and a 0.75 numerical aperture. Images were taken using an Olympus Colorview III camera with AnalySIS^B image processing software.

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Immunostaining of Vein Sections

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Vein samples were embedded in Frozen Section Medium (Richard-Allan Scientific Neg 50, Thermo Scientific) and frozen in dry ice-cooled isopenthane. Frozen tissue blocks were stored at —80C. The samples were cut in 8 um thick sections on a CryoStar™ NX70 Cryostat (Thermo Scientific™), mounted on SuperFrost Plus Adhesion slides and immediately post-fixed for 30 s in cold 95% ethanol. Slides were stored at —20C awaiting immunostaining.
Sections were immunostained for the presence of CD31 (1:50; Abcam #ab9498), von Willebrand factor (1:500; Abcam #ab6994), VEGFR-2 (1:200; Acris Antibodies TA337222), alfa smooth muscle actin (1:500, Abcam #ab7817), CD41 (1:1000; Abcam #ab11024) and fibrinogen (1:250; Abcam #ab118488). Antibodies were diluted in blocking buffer (5% goat serum/3% BSA in PBS), and slides were incubated at 4 °C overnight. Negative controls were made omitting the primary antibody. The secondary antibodies used were goat anti-rabbit IgG conjugated to Alexa 488 and goat anti-mouse IgG conjugated to Alexa 594 (Life Technologies), used at 1:500. Each immunostaining procedure was performed in triplicate and repeated at least twice per vein sample.
The stained sections were mounted with prolong gold antifade (Invitrogen), containing DAPI for nuclear staining. Imaging was done using an upright Nikon Eclipse E600 microscope equipped with an Olympus ColorView III camera.

Rambøl M.H., Hisdal J., Sundhagen J.O., Brinchmann J.E, & Rosales A. (2018). Recellularization of Decellularized Venous Grafts Using Peripheral Blood: A Critical Evaluation. EBioMedicine, 32, 215-222.

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