Piceatannol

Manufactured by Selleck Chemicals

Sourced in United States, China

Piceatannol is a naturally occurring polyphenol compound. It serves as a research tool for studies related to cellular signaling and biological processes.

Automatically generated - may contain errors

Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) U73122, by Cayman Chemical (2 mentions) Sb203580, by InvivoGen (2 mentions) U0126, by Bio-Techne (2 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Caspase 3 activity assay kit, by Beyotime (1 mentions) Hoechst 33258, by Beyotime (1 mentions) Trap stain kit, by Merck Group (1 mentions) M csf, by R&D Systems (1 mentions) Rankl, by R&D Systems (1 mentions) Raw264.7 cells, by Procell (1 mentions) Acarbose, by Merck Group (1 mentions) α glucosidase from saccharomyces, by Merck Group (1 mentions) Tcs sp5, by Leica (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Curcumin, by Selleck Chemicals (1 mentions) Resminostat, by Selleck Chemicals (1 mentions)

10 protocols using piceatannol

Osteoclast Differentiation Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were purchased from GIBCO (Invitrogen Corp., Carlsbad, CA, USA). The M-CSF and RANKL were obtained from R & D Systems (Minneapolis, MN, USA). The cell counting kit-8 was obtained from Phygene (Fuzhou, China). The caspase-3 activity assay kit and Hoechst 33258 were obtained from Beyotime Biotechnology (Shanghai, China). TRAP stain kit was purchased from Sigma-Aldrich (USA). RAW264.7 cells were obtained from Procell (Wuhan, China). Piceatannol (PIC) was acquired from Selleck Chemicals (USA) and dissolved in dimethyl sulfoxide (DMSO) and then diluted to the required working concentrations in complete culture medium. All antibodies used in this study were obtained from Cell Signalling Technology (Danvers, MA).

Yan L., Lu L., Hu F., Shetti D, & Wei K. (2019). Piceatannol attenuates RANKL-induced osteoclast differentiation and bone resorption by suppressing MAPK, NF-κB and AKT signalling pathways and promotes Caspase3-mediated apoptosis of mature osteoclasts. Royal Society Open Science, 6(6), 190360.

+ Open protocol

+ Expand

Yeast α-Glucosidase Enzymatic Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

α-Glucosidase from Saccharomyces cerevisiae (EC 3.2.1.20, 50.3 U mg⁻¹), 4-nitrophenyl-β-d-glucopyranosiduronic acid (pNPG), and acarbose were obtained from Sigma-Aldrich (St, Louis, USA). p-Nitrophenol (pNP) was purchased from Aladdin Biotechnology (Shanghai, China). Piceatannol was purchased from Selleck Chemicals (USA). All other chemicals were of analytical reagent grade, and their aqueous solutions were prepared with freshly ultrapure water.

Jiang L., Wang Z., Wang X., Wang S., Cao J, & Liu Y. (2020). Exploring the inhibitory mechanism of piceatannol on α-glucosidase relevant to diabetes mellitus. RSC Advances, 10(8), 4529-4537.

+ Open protocol

+ Expand

Piceatannol Inhibits Schistosoma japonicum Reproduction

Check if the same lab product or an alternative is used in the 5 most similar protocols

Piceatannol (3, 4, 39, 59-tetrahydroxy-trans-stilbene; Selleck Chemicals, Shanghai, China) was dissolved in dimethyl sulfoxide (DMSO). In each experimental group, 15 adult couples of S. japonicum were cultured in 3 ml of medium and treated with one of three different concentrations of Piceatannol (35 mM, 70 mM, or 100 mM). The medium with inhibitor was changed every 24 h for 7 days. During this time, the viability of parasites, worm pairing, and the number of eggs in the culture medium were recorded. For the morphological analysis, Schistosoma worm pairs were fixed in a solution of alcohol (95%), formalin (3%), and glacial acetic acid (2%) (AFA) for at least 24 h. Parasites were stained in hydrochloric acid-carmine dye (Ourchem, Shanghai, China) for 17 h, and then destained in acidic 70% ethanol. The parasites were dehydrated in a graded ethanol series (70%, 90% and 100%). Parasites were cleared in 50% xylene diluted in ethanol and 100% xylene for 1 min each, and then mounted onto slides with neutral gum, sealed with cover glass, and laid flat to dry. The morphology of the reproductive organs of parasites was observed with a confocal laser scanning microscope (CLSM, Leica TCS SP5, Mannheim, Germany) using an emission wavelength of 488 nm and a scan excitation of 30%. Images were captured and stored at 1024 × 1024 pixels.

Ding H., Liu F., Zhu L., Wu F., Liu Q., He S., Shao W., Du Y., Ren C., Shen J, & Liu M. (2017). Tyrosine kinase 4 is involved in the reproduction of the platyhelminth parasite Schistosoma japonicum. Parasites & Vectors, 10, 498.

+ Open protocol

+ Expand

Comprehensive Chemical Reagents for Cellular Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols

Resveratrol, piceatannol, resminostat, entinostat, mocetinostat, vorinostat, curcumin, garcinol, anacardic acid and Tip60i were purchased from Selleckchem (Houston, TX, USA). MB-3 and BMS-345541 were purchased from MilliporeSigma (Burlington, MA, USA). Pterostilbene and myricetin were from LKT Laboratories (St. Paul, MN, USA) and trimethoxy-Resveratrol (trans-3,5,4′-trimethoxystilbene) was from Cayman Chemical Co. (Ann Arbor, MI, USA). Stock solutions of the chemicals were prepared based on the information provided by the manufacturer and maintained at −20°C. The antibodies for human PD-L1 (E1L3N, 13684), p38 MAPK (D13E1, 8690), NF-κB p65 (D14E12, 8242), γH2AX (20E3, 9718), cleaved caspase 3 (D3E9, 9579), IRF-1 (D5E4, 8478) and rabbit IgG isotype monoclonal antibody (DA1E, 5742) conjugated to PE were obtained from Cell Signaling Technology (Beverly, MA, USA). c-Myc antibody (9E10, sc-40) was obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Fetal bovine serum, RPMI-1640, DMEM, streptomycin and penicillin were from Gibco/Thermo Fisher Scientific (Waltham, MA, USA). All other chemicals and solvents were of analytical grade.

Lucas J., Hsieh T.C., Halicka H.D., Darzynkiewicz Z, & Wu J.M. (2018). Upregulation of PD-L1 expression by resveratrol and piceatannol in breast and colorectal cancer cells occurs via HDAC3/p300-mediated NF-κB signaling. International Journal of Oncology, 53(4), 1469-1480.

+ Open protocol

+ Expand

Mouse Dendritic Cell Activation Assay

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse bone marrow derived DCs were grown as previously described (Goodridge et al., 2009 (link)). DCs were stimulated overnight with PFA-fixed yeast (10 yeast/cell), PFA-fixed hyphae (same dry weight as yeast preparation), 3,6,15, 25, and 45 μm polystyrene beads (10, 2.5, 0.4, 0.144, 0.044 beads/cell respectively). For inhibitor studies, cells were treated with U-73122 (Cayman chemical), U0126 (Tocris Bioscience), SB203580(Invivogen) or piceatannol (Selleck Chemicals) 1 h prior to addition of stimuli.

Oh S., Li K., Prince A., Wheeler M.L., Hamade H., Nguyen C., Michelsen K.S, & Underhill D.M. (2022). Pathogen size alters C-type lectin receptor signaling in dendritic cells to influence CD4 Th9 cell differentiation. Cell reports, 38(13), 110567.

+ Open protocol

+ Expand

Mouse Dendritic Cell Activation Assay

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

Acupuncture for Intracerebral Hemorrhage

Check if the same lab product or an alternative is used in the 5 most similar protocols

After successful model establishment, in the acupuncture group, Baihui (DU20) (specific location: head between the middle ears) and Qubin (GB7) (specific location: leading edge of ear root) points were identified (Li, 2007). Hair was cut around the Baihui (DU20) point, and a 0.30 × 25 mm acupuncture needle (Hua Tuo Brand; Suzhou Medical Appliance, Suzhou, China) used to pierce from the epicranial aponeurosis to the Qubin (GB7) point in the bottom right direction. The needle pierced to a depth of 15 mm, and was then rotated at 200 r/min for 5 minutes. For each 30-minute session, needling at 200 r/min was performed for three session, each lasting for 5 minutes.
In the sham and ICH groups, rats were fixed on the frame for 30 minutes without any operation.
In the piceatannol group, rats were intraperitoneally injected with piceatannol (34.62 mg/kg) (license No. 10083-24-6; Selleck, Shanghai, China) at 1 hour after model establishment.

Liu X.Y., Dai X.H., Zou W., Yu X.P., Teng W., Wang Y., Yu W.W., Ma H.H., Chen Q.X., Liu P., Guan R.Q, & Dong S.S. (2018). Acupuncture through Baihui (DU20) to Qubin (GB7) mitigates neurological impairment after intracerebral hemorrhage. Neural Regeneration Research, 13(8), 1425-1432.

+ Open protocol

+ Expand

Inhibiting TREM1 and SYK in SAH

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

As previously reported, TREM1 inhibitory peptide LP17 (LQVTDSGLYRCVIYHPP) was chemically synthesized from GenScript, China (9 (link), 25 (link)). To inhibit TREM1, vehicle or LP17 (1 mg/kg) dissolved in ultrapure water was administered intranasally 1 h post-modeling (9 (link)). Recombinant TREM-1 (GenScript, China) was intranasally administered at 1 h after SAH at the dosage of 3 μg/mouse (9 (link)). To inhibit SYK, mice were injected intraperitoneally with piceatannol (20 mg/kg, Selleck, USA) 1 h post-modeling (34 (link)).

Wu X., Zeng H., Xu C., Chen H., Fan L., Zhou H., Yu Q., Fu X., Peng Y., Yan F., Yu X, & Chen G. (2021). TREM1 Regulates Neuroinflammatory Injury by Modulate Proinflammatory Subtype Transition of Microglia and Formation of Neutrophil Extracellular Traps via Interaction With SYK in Experimental Subarachnoid Hemorrhage. Frontiers in Immunology, 12, 766178.

+ Open protocol

+ Expand

THCE Cells: Culturing and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

The THCE cells were kindly given by Xiamen eye center. Delbeccon’s modified Eagle’s medium(DMEM), Fetal Bovine Serum(FBS) and 0.25 % trypsin/0.03 % EDTA solution were purchased from Gibco (San Diego, California, USA); Dimethylsulfoxide (DMSO) was purchased from Solarbio (Beijing, China); Sabouroud medium was purchased from Babio biotech (Jinan, China); RNAiso Plus and reverse transcriptase polymerase chain reaction (RT-PCR) kits and SYBR® Premix Ex Taq™ (Tli RNaseH Plus) were purchased from TaKaRa (Dalian, China); Primary antibodies against phospho-Syk and Syk came from Cell Signaling Technology (Danvers, MA). A mouse antibody against GAPDH, Bicinchoninic Acid Assay, ECL Western Blotting Detection Reagent were from Beyotime (Shanghai, China); The secondary antibodies were from Cwbiotech (Beijing, China); Syk inhibitors, PRT062607 and Piceatannol, were purchased from Selleck Chemicals (Houston, USA); Phenylmethylsulphonylfluoride (PMSF) and radio immunoprecipitation assay (RIPA) lysis buffer were purchased from Solarbio (Beijing, China).

Liu Y., Zhao G., Lin J., Li C., Li Q., Che C., Wang Q, & Hu L. (2015). The role of Syk signaling in antifungal innate immunity of human corneal epithelial cells. BMC Ophthalmology, 15, 55.

+ Open protocol

+ Expand

Phagocytosis of Aged Red Blood Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

BMDMs were grown in 20% L929-conditioned media for 7 days and then were replated at a density of 2 × 10⁶ cells per well. BMDMs were stimulated overnight with zymosan (10 μg/ml) and LPS (10 ng/ml). RBCs were collected in an EDTA-coated tube, labeled with CFSE, and aged in Hepes buffer [10 mM Hepes, 140 mM NaCl, and 0.1% BSA (pH 7.4)] containing calcium (2.5 mM) and Ca2⁺ ionophore (0.5 μM, A23187). RBCs were aged for 16 hours at 37°C. BMDMs were washed twice with PBS before aged RBCs were added at a concentration of 3 × 10⁷ cells/ml (52 (link)). BMDMs were incubated with either 20 μM LY294002 (S1105, Selleckchem) or 100 μM piceatannol (S3026, Selleckchem) for 1 hour before the addition of aged RBCs. BMDMs were incubated with RBCs at 37°C for 1 hour and were then washed twice with PBS. BMDMs were harvested, and phagocytosis was assessed by flow cytometry. For in vitro phagocytosis assays using primary phagocytes, splenocytes were isolated and rested for 4 hours to allow cells to adhere. Cells were incubated with 30 μM JSH-23 (S7351, Selleckchem) for 4 hours while resting. Primary cells were then incubated with aged RBCs for 3 hours, and RNA was isolated.

Bennett L.F., Liao C., Quickel M.D., Yeoh B.S., Vijay-Kumar M., Hankey-Giblin P., Prabhu K.S, & Paulson R.F. (2019). Inflammation induces stress erythropoiesis through heme-dependent activation of SPI-C. Science signaling, 12(598), eaap7336.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!