Cell viability was determined by using a CCK-8 assay kit (Jiancheng, Nanjing, China) in 96-well plates according to the manufacturer's instructions. 10 μl of CCK-8 reagent was added to the culture medium at each well and then continuously incubated for 3 h in darkness at 37°C. The absorbance was determined at 450 nm using a PerkinElmer microplate reader (PerkinElmer VICTOR 1420, USA).
Victor 1420
Manufactured by PerkinElmer
Sourced in United States
The Victor 1420 is a multi-mode microplate reader that can perform a variety of detection methods, including absorbance, fluorescence, and luminescence. It is designed for use in life science research and drug discovery applications.
Automatically generated - may contain errors
Lab products found in correlation
Cck 8 assay kit, by Nanjing Jiancheng (3 mentions)
Cell counting kit 8 assay, by Dojindo Laboratories (2 mentions)
Bright glo luciferase detection reagent, by Promega (2 mentions)
Ly2109761, by Selleck Chemicals (1 mentions)
Cck 8 kit, by Apexbio (1 mentions)
Elf97 endogenous phosphatase detection kit, by Thermo Fisher Scientific (1 mentions)
Cell counting kit 8 cck 8 assay kit, by Nanjing Jiancheng (1 mentions)
Facscalibur, by BD (1 mentions)
Lactate colorimetric fluorometric assay kit, by Abcam (1 mentions)
Synergy h1 microplate reader, by Agilent Technologies (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Dual luciferase reporter assay system, by Promega (1 mentions)
Cck 8, by Nanjing Jiancheng (1 mentions)
Cck 8, by PerkinElmer (1 mentions)
Toluidine blue, by Merck Group (1 mentions)
Adp glo kinase assay kit, by Promega (1 mentions)
Cck 8 kit, by Biosharp (1 mentions)
Dmem glutamax 1, by Thermo Fisher Scientific (1 mentions)
Delfia assay buffer, by PerkinElmer (1 mentions)
Delfia enhancement solution, by PerkinElmer (1 mentions)
28 protocols using victor 1420
1
Cell Viability Assay with CCK-8
2
Imatinib Drug Delivery for Cancer Cell Inhibition
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The effect of imatinib mesylate in the form of free drug solution and INPs on cell proliferation was determined using MTT assay. MCF-7 cells were plated in fat-bottom 96-well plates at 5,000 cells per well. After incubation for 24 hours at 37°C, the culture medium was removed and replaced with 100 μL fresh medium containing free drug (0.148, 0.295, 0.59, and 1.18 μg), and INPs (6.56, 13.11, 26.22, and 52.44 μg); both free drug and INP additions were equivalent to final imatinib concentrations of 2.5, 5, 10, and 20 μM, respectively. Corresponding controls were maintained by adding 100 μL medium for free drug control and 100 μL medium containing 52.44 μg empty nanoparticles for INP control. After 48 hours, medium from the treatments and controls were replaced with 100 μL medium containing 50 μg MTT and were incubated for 1 hour. Then, 100 μL sodium dodecyl sulfate (SDS) solution (20% w/v, water:DMF at a 1:1 ratio, pH 4.7) was added to each well and further incubated for 24 hours to dissolve the formazan crystals formed. The absorbance was read at 570 nm in an enzyme-linked immunosorbent assay (ELISA) plate reader (Victor 1420; PerkinElmer Inc, Waltham, MA, USA). The amount of MTT that is converted to formazan after incubation with cells corresponds to the number of viable cells. The half-maximal inhibitory concentration (IC50) was determined by nonlinear regression analysis.
3
Luminescence-based Sulfide Detection Assay
4
HUVEC Viability Evaluation Using CCK-8 Assay
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The Cell Counting Kit (CCK)-8 assay (Dojindo Laboratories, Kumamoto, Japan) was used to assess cell viability. Briefly, HUVECs were seeded in 96-well plates containing DMEM containing 10% FBS. The TGF-β inhibitor LY2109761 (S2704, Selleckchem, Houston, TX, USA) was reconstituted in DMSO and stored at -80° C. First, HUVECs at the beginning of the OGD period were treated with LY2109761 at different concentrations (0, 0.5, 1, 1.5, and 2 μM) [45 (link)] and Coicis Semen at different concentrations (10, 400, 500, 600, and 700 μM) [24 (link)] after OGD/RX to choose the appropriate doses. Then, the medium was removed, and 10 μl of CCK-8 solution was added to each well. After 2 h of incubation at 37° C, the absorbance at 450 nm was measured using an automatic microplate reader (PerkinElmer Victor 1420, Alburgh, VT, United States).
5
Assessing HT-29 Cell Viability
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CCK-8 kits (K1018, APExBIO, United States) were used to assess the viability of HT-29 cells. Cells at approximately 90% confluence in 96-well plates were treated with various doses of luteolin for 24 h. A total of 10 μL of CCK-8 reagent was added to each well and incubated for 1 h at 37°C in the dark. A PerkinElmer microplate reader was used to measure the absorbance at 450 nm (PerkinElmer VIC-TOR 1420, United States).
6
ALP Activity Measurement in Encapsulated Cells
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ALP activity was assesed using the ELF 97 Endogenous Phosphatase Detection Kit (Molecular Probes, Leiden, The Netherlands) according to the manufacturers protocol. At day 9 post encapsulation beads were dissolved in citrate (50mM) and cells were fixated and permeabilized before addition of the phosphatase substrate. Fluorescence was measured using a multilabel counter (Victor 1420, Perkin Elmer).
7
Quantifying Cell Viability with CCK-8
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Cell Counting Kit (CCK)-8 assay (Dojindo Laboratories, Kumamoto, Japan) was used to assess the cell viability. Briefly, neuronal cell line HT-22 and microglia cell line BV2 were seeded in 96-well plates with DMEM containment 10% FBS. Cells were treated with LPS (1 μg/mL) and different concentrations (10, 30, 50, 100, and 150 mM) of meisoindigo at the beginning of OGD. The optimal dose of LPS was selected based on previous studies (Chen et al., 2018 (link)). Then the medium was removed, 10 μl of CCK-8 solution was subsequently added to each well. After 2 h of incubation at 37°C, the absorbance at 450 nm was measured using an automatic microplate reader (PerkinElmer Victor 1420, Alburg, VT, United States).
8
Cell Viability Assessment Using CCK-8 Assay
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The Cell Counting Kit-8 (CCK-8) Assay Kit (Jiancheng, Nanjing, China) was used to assess cell viability. HK-2 cells were incubated in 96-well plates, followed by the addition of 10 μl of CCK-8 reagent per well. The plates were incubated for 3 h in the dark. A PerkinElmer Microplate reader (PerkinElmer Victor 1420, USA) was used to measure the absorbance at 450 nm. The percentage of cell viability was determined as follows: (Treatment group optical density/control group optical density) × 100%.
9
SARS-CoV-2 Entry Inhibition Screening
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HEK293T-ACE2-GFP cells were seeded in white, transparent bottom 96-well microplates at 20,000 cells per well in 100 μL growth medium and incubated at 37 °C with 5% CO2 overnight (~16 h). The growth medium was carefully removed, and 50 μL PP or PP-containing compounds were added to each well. The plates were then spinoculated by centrifugation at 1500 rpm (453× g) for 45 min and incubated for 24 h (48 h for Calu-3 cells) at 37 °C, 5% CO2 to allow cell entry of PP and the expression of luciferase. After incubation, the supernatant was carefully removed. Then 50 μL/well of Bright-Glo luciferase detection reagent (Promega) was added to assay plates and incubated for 5 min at room temperature. The luminescence signal was measured by a Victor 1420 plate reader (PerkinElmer). For ACE2-GFP cells, the GFP signal was also determined by the plate reader. Data were normalized with wells containing PP but no compound as 100%, wells mock-treated with phosphate buffer saline (PBS) as 0%, and the ratio of luciferase to the corresponding GFP intensity was calculated.
10
Measuring Plasma Oxytocin Levels in Mice
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