Sequencing data was processed as previously described17 (link),63 (link). Briefly, ChIP-seq reads were aligned to hg19 using bowtie58 (link) (v1.1.0) allowing up to 3 mismatches, discarding ambiguous and clonal reads. All histone ChIP-seq peaks were called using PeakRanger67 (v1.16). Broad domains of lamins (LADs), H3K9me3 and H3K27me3 were called using the enriched domain detector (EDD) for identification of wide genomic enrichment domains68 (link).
Truseq chip library prep kit
The TruSeq ChIP Library Prep Kit is a laboratory equipment product designed for the preparation of DNA libraries for chromatin immunoprecipitation (ChIP) sequencing. The kit provides the necessary reagents and protocols to convert DNA samples into indexed libraries suitable for sequencing on Illumina platforms.
Lab products found in correlation
15 protocols using truseq chip library prep kit
Chromatin Immunoprecipitation Sequencing Protocol
Sequencing data was processed as previously described17 (link),63 (link). Briefly, ChIP-seq reads were aligned to hg19 using bowtie58 (link) (v1.1.0) allowing up to 3 mismatches, discarding ambiguous and clonal reads. All histone ChIP-seq peaks were called using PeakRanger67 (v1.16). Broad domains of lamins (LADs), H3K9me3 and H3K27me3 were called using the enriched domain detector (EDD) for identification of wide genomic enrichment domains68 (link).
ChIP-seq Analysis of Chromatin Epigenetic Marks
DNA Sequencing Library Preparation
Affinity-seq for Identifying Protein-DNA Interactions
SNAI2 ChIP-seq Protocol for Gene Regulation
Chromatin Immunoprecipitation with Sonication
ChIP-seq and In Situ Biotinylation-seq in Drosophila
ChIP-seq Analysis of Chromatin Epigenetic Marks
Nuclei Isolation and DNA Extraction
ChIP-seq Data Processing and Analysis
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