The prepared sections were blocked with 3% (w/v) bovine serum albumin (BSA; ZSbio) in PBST (0.1% [v/v] Triton X-100 in PBS) for 1 h at room temperature and then incubated with the following primary antibodies overnight at 4°C: anti-Sycp3 (1:200, Abcam, Cambridge, MA, USA), anti-synaptonemal complex protein 1 (Sycp1; 1:200, Abcam), anti-Vasa (1:500, Abcam), anti-c-kit (1:500, Abcam), anti-Shp2 (1:200, Santa Cruz Biotechnology), anti-Plzf (1:500, Santa Cruz Biotechnology), anti-cleaved caspase 3 (1:200, Cell Signaling Technology, Boston, MA, USA), anti-Dmc1 (1:200, Abcam), anti-Smc3 (1:500, Abcam), and anti-DNA repair recombinase rad51 (Rad51; 1:200, Invitrogen). After being washed three times with PBST, the samples were incubated with the following secondary antibodies at a 1:200 dilution for 1 h at 37°C: Alexa Fluor 594/488-labeled anti-rabbit or anti-mouse IgG (YEASEN, Shanghai, China). The slides were subsequently washed three times in PBST and mounted with Vectashield containing 4’-6-diamidino-2-phenylindole (DAPI; Vector Laboratories, Burlingame, CA, USA).
Anti vasa
Manufactured by Abcam
Sourced in United States
Anti-Vasa is a lab equipment product that functions as an antibody. It is used for the detection and analysis of the Vasa protein, which is a key regulator of germ cell development in various organisms.
Automatically generated - may contain errors
Lab products found in correlation
Anti plzf, by Santa Cruz Biotechnology (2 mentions)
Anti c kit, by Abcam (2 mentions)
Bovine serum albumin bsa, by ZSGB-BIO (1 mentions)
Anti sycp3, by Abcam (1 mentions)
Sycp1, by Abcam (1 mentions)
Anti shp2, by Santa Cruz Biotechnology (1 mentions)
Anti dmc1, by Abcam (1 mentions)
Anti smc3, by Abcam (1 mentions)
Vectashield containing 4 6 diamidino 2 phenylindole dapi, by Vector Laboratories (1 mentions)
Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Nbp2 16109, by Novus Biologicals (1 mentions)
Alexa fluor 488, by Jackson ImmunoResearch (1 mentions)
Ab9045, by Abcam (1 mentions)
Ab1220, by Abcam (1 mentions)
Anti flag clone m2, by Merck Group (1 mentions)
Anti c kit, by BioLegend (1 mentions)
Anti h3k9me2, by Abcam (1 mentions)
Anti epcam clone g8, by Thermo Fisher Scientific (1 mentions)
Anti tra98, by Abcam (1 mentions)
Anti sox2, by Abcam (1 mentions)
4 protocols using anti vasa
1
Immunofluorescence Staining of Germ Cell Markers
2
Zebrafish Embryonic Protein Localization
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Zebrafish embryos at different stages of development were fixed overnight in cold 4% paraformaldehyde-PBS (PFA). Embryos were then dehydrated with methanol for storage at −20°C and rehydrated, washed in PBST [PBS+0.1% Tween 20], permeabilized with cold acetone (20 s), blocked with goat serum for at least 1 h at room temperature. Thereafter, they were incubated with rabbit anti-P54 serum (1:2000), rabbit pre-immune serum, rabbit anti-Dcp2 (Novus Biologicals, NBP2-16109; 1:2000), rabbit anti-TIAL-1 (Novus Biologicals ,NBP1-79932; 1:2000), rabbit anti-phosphorylated non-muscle myosin (NMII-p) (Cell Signaling, 3671) or rabbit anti-DDX4 (anti-Vasa) (Abcam, ab13840), these were diluted in PBST and incubated overnight at 4°C. Labeling was detected using secondary antibody (1:1000) goat anti-rabbit IgG conjugated with Alexa Fluor 488 (Jackson ImmunoResearch, 111-545-003), and nuclei were counterstained with DAPI (4′,6-diamidino-2-phenylindole).
3
Antibody Immunoblotting for Stem Cell Markers
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The following antibodies were used were used for immunoblotting. Anti-SOX9 (Merck Millipore, AB5535), anti-HSD3b (TransGenic, KO607), anti-TRA98 (Abcam, ab82527), anti-FLAG (clone M2, Sigma, A8592), anti-VASA (Abcam, ab13840), anti-EPCAM (clone G8.8, eBioscience, no. 14-5791-85), anti-FOXO1 (CST, no. 2880), anti-PLZF (Santa Cruz, H300), anti-c-KIT (BioLegend, no. 105803), anti-JMJD1B (Abvnova, PAB15833), anti-H3K9me1 (Abcam, ab9045), Anti-H3K9me2 (clone 6D11) (Kimura et al., 2008 (link)), anti-H3K9me3 (clone 2F3) (Kimura et al., 2008 (link)), anti-JMJD1A (clone F0618) (Abe et al., 2015 (link)), and anti-JMJD1B (Abvnova, PAB15833). Anti-H3K9me2 (Abcam, Ab1220) was used for ChIP analysis.
4
Characterization of Pluripotent Stem Cells
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Cells from different culture time points were grown on or adhered to coverslips. The cells were subsequently fixed with 4% paraformaldehyde and blocked in 1× PBS with 5% BSA and 0.05% Triton-X-100 and were subsequently incubated overnight at 4°C with various primary antibodies, including anti-Oct4 (1:200; rabbit origin; AbCam), anti-Sox2 (1:200; rabbit; AbCam), anti-c-Kit (1:80; rabbit; AbCam), anti-Vasa (1:200; rabbit; AbCam), anti-DAZL (1:150; mouse; AbCam), anti-Nanos3 (1:200; rabbit; AbCam), anti-GDF9 (1:200; rabbit; AbCam), or SCP3 (1:400; rabbit; AbCam). Next, the cells were incubated with Cy3 fluorescent dye-conjugated secondary antibodies (Jackson). The cell nuclei were counterstained with 4, 6-diamidino-2-phenylindole (DAPI; Invitrogen).
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