Paraffin-embedded sections were first de-waxed and rehydrated prior to antigen retrieval. CGNPs were grown on poly-DL-ornithine-coated glass coverslips as described previously (10 (link)). The cells were fixed with 4% paraformaldehyde for 20 min. Sections and cells were analyzed by immunofluorescence according to standard methods. Antibodies used for immunofluorescence were YB-1 (Cell Signaling Cat# 4202S/Santa Cruz Biotechnology Cat# sc-4358), or PCNA (Calbiochem, Temecula, CA, USA; Cat# NA03), or Ki67 (Vector Labs, Burlingame, CA, USA; Cat#VP-K451), GFP (Abcam, Cambridge, MA; Cat#ab290), mouse and rabbit IgG (Life technologies; Cat# A11032, A11034, A11012, A11017).
Mouse and rabbit igg
Manufactured by Thermo Fisher Scientific
Sourced in United States
Mouse and rabbit IgG are antibodies commonly used in various laboratory techniques, such as Western blotting, immunoprecipitation, and enzyme-linked immunosorbent assays (ELISA). They serve as secondary antibodies to detect and quantify target proteins or antigens in biological samples.
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Lab products found in correlation
2 protocols using mouse and rabbit igg
1
Immunofluorescence Staining of Cells
2
Immunofluorescence Staining of Cells
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Paraffin-embedded sections were first de-waxed and rehydrated prior to antigen retrieval. CGNPs were grown on poly-DL-ornithine-coated glass coverslips as described previously (10 (link)). The cells were fixed with 4% paraformaldehyde for 20 min. Sections and cells were analyzed by immunofluorescence according to standard methods. Antibodies used for immunofluorescence were YB-1 (Cell Signaling Cat# 4202S/Santa Cruz Biotechnology Cat# sc-4358), or PCNA (Calbiochem, Temecula, CA, USA; Cat# NA03), or Ki67 (Vector Labs, Burlingame, CA, USA; Cat#VP-K451), GFP (Abcam, Cambridge, MA; Cat#ab290), mouse and rabbit IgG (Life technologies; Cat# A11032, A11034, A11012, A11017).
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