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Eos 60d digital camera

Manufactured by Canon
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Sourced in Japan

The Canon EOS 60D is a digital single-lens reflex camera. It features a 18.0-megapixel CMOS sensor, DIGIC 4 image processor, and a 3.0-inch vari-angle LCD screen. The camera is capable of recording full HD 1080p video at 30 frames per second.

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Lab products found in correlation

Ef s18 55 is lens, by Canon (7 mentions) Axiocam hrm camera, by Zeiss (2 mentions) Ef s60 mm f2, by Canon (2 mentions) Stemi dv4, by Zeiss (2 mentions) Axio zoom v16 microscope, by Zeiss (2 mentions) Mvx10 microscope, by Olympus (2 mentions) Et 640 30x, by Chroma Technology (2 mentions) Hq700 75m hcar, by Chroma Technology (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Ez4 hd microscope, by Leica camera (1 mentions) Sas software v9, by SAS Institute (1 mentions) Trastuzumab, by Genentech (1 mentions) Sz61 microscope, by Olympus (1 mentions) Leica s8 apo, by Leica camera (1 mentions) Dfc450, by Leica camera (1 mentions) Ivis lumina xrms series 3, by PerkinElmer (1 mentions) Axiozoom, by Zeiss (1 mentions) Zen software, by Zeiss (1 mentions) Nanozoomer slide scanner, by Hamamatsu Photonics (1 mentions) 1260 series hplc uv system, by Waters Corporation (1 mentions)

19 protocols using eos 60d digital camera

1

Multimodal Imaging of Mouse Anatomy

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Macroscopic images of the specimens were captured using an EOS 60D digital camera (Canon, Tokyo) equipped with a macro lens (EF-S60 mm F2.8, Canon, Tokyo). The HE-stained skin section histological images were captured with the NanoZoomer slide scanner (Hamamatsu Photonics, Hamamatsu, Japan). Stained bone specimens were observed with a binocular microscope (Stemi DV4, Zeiss, Oberkochen, Germany) and photographed using the EOS 60D digital camera (Canon, Tokyo) equipped with a macro lens (EF-S60 mm F2.8, Canon, Tokyo). An Axio Zoom.V16 microscope equipped with an AxioCam HRm camera (Zeiss, Oberkochen, Germany) was used for fluorescent observation. The stained adult mouse limbs were examined with a fluorescence zoom microscope (Axio Zoom, Carl Zeiss Microscopy, GmbH). Alexa Fluor 594 (Ex. 559–585/Em. 600–690 nm) was used for alizarin red S staining and Alexa Fluor 488 (Ex. 450–490/Em. 500–550 nm) was used to detect the non-specific signals from the muscles and tendons. The 3D images were reconstructed using ZEN software (Carl Zeiss Microscopy, GmbH) from Z-stacked images. A radiogram of the extremities of the CIA mice under anesthesia by isoflurane inhalation was obtained using the IVIS Lumina XRMS Series III (IVIS Lumina XRMS Series III, PerkinElmer, MA) under anesthesia by isoflurane inhalation.
Kariyama N., Sakata-Haga H., Tsukada T., Shimada H., Taniguchi M, & Hatta T. (2021). Rapid bone staining with hair removal (RAP-B/HR): a non-destructive and rapid whole-mount bone staining protocol optimized for adult hairy mice. Scientific Reports, 11, 1950.
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2

Subcellular Localization and Transcriptional Activation of ZjZFN1

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Agrobacterium tumefaciens EHA105 transformed with the 35S::ZjZFN1:YFP fusion construct were used to transform N. benthamiana to reveal the subcellular localization of ZjZFN1 using the transient overexpression method (Sparkes et al., 2006 (link)). After darkness induction for 48 h, the cells from the lower epidermis of N. benthamiana leaf cells were monitored and photographed under a SP-5 laser confocal scanning microscope (Leica, Mannheim, Germany). 1 μg/mL DAPI (Sigma-Aldrich, Munich, Germany) was used to show the nuclear.
The pGBKT7-ZjZFN1 construct was transformed into Saccharomyces cerevisiae Y2HGold competent cells using the LiAc method (Liu et al., 2016 (link)) to investigate its transcriptional activation ability. Firstly, transformed Y2HGold cells (pGBKT7 was use as control) were grown on synthetic defined (SD) medium without tryptophan (SD/-Trp). After colony-PCR verification, the positive clones were grown on SD medium without tryptophan, histidine and adenine (SD/-Trp-His-Ade) at 30°C for about 48 h. Yeast growth phenotypes were then photographed with an EOS 60D digital camera (Canon, Tokyo, Japan).
Teng K., Tan P., Guo W., Yue Y., Fan X, & Wu J. (2018). Heterologous Expression of a Novel Zoysia japonica C2H2 Zinc Finger Gene, ZjZFN1, Improved Salt Tolerance in Arabidopsis. Frontiers in Plant Science, 9, 1159.
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3

Quantifying Yeast Colony Sizes

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Colony sizes of yeast strains after transformation with polyQ proteins were documented by taking photographs of whole petri dishes. A Canon EOS 60D digital camera mounted on a repro stand was used for this purpose.
Papsdorf K., Kaiser C.J., Drazic A., Grötzinger S.W., Haeßner C., Eisenreich W, & Richter K. (2015). Polyglutamine toxicity in yeast induces metabolic alterations and mitochondrial defects. BMC Genomics, 16(1), 662.
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4

Dianthus Seed Micromorphology Evaluation

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Dianthus specimens were thoroughly evaluated using the relevant literature (Fenzl 1842 , Boissier 1849 , Tchihatcheff 1860 , Reeve 1967 , Bojňanský and Fargašová 2007 ) and the specimens present in GAZI herbarium. Furthermore, the specimens collected from the Bilecik population included the material needed for the seed micromorphology studies. Images were taken using a Canon EOS 60D digital camera, and the seed surface micromorphology was visualized using a LEO 440 scanning electron microscope. Normal visualization of the specimens was carried out using a Leica EZ4 HD microscope. The vegetative characters were measured using a ruler with 0.5 mm accuracy; floral characters were measured using an ocular micrometer. Seed morphology is described following the nomenclature of Bojňanský and Fargašová (2007) . Specimens have been deposited in the herbaria of Gazi (GAZI) and Ankara Universities (ANK).
Hamzaoğlu E., Koç M, & Aksoy A. (2015). Dianthus aticii, a new species from Turkey (Caryophyllaceae). PhytoKeys.
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5

Automated Lid Wiper Evaluation Using Slit Lamp

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Prior to image management and LWE detection, photographs of the everted lid (resolution of 2000*1000 digitized on 8 bits, 12× magnification, Haag-Streit BI900 LED Slit Lamp system and Canon EOS 60D digital camera) were captured in raw mode, and then converted into tiff-format images. The ADCIS software is designed to automatically detect LWE when using LG and NaFl vital dyes. Once this dyed area was detected, the software automatically segments the area and processes a series of computed measures (shape and intensity of the automatically detected regions). As LWE may have different presentations (continuous and non-continuous staining), the calculated area of lid wiper staining (mm2) used for analysis includes all stained regions as well as the Line of Marx (mean area 2.06 mm2). This approach is consistent with previous studies using alternative semi-automated methodologies.9 (link),17 (link),18 (link) Statistical analyses were performed using SAS software v9.4 (Cary, NC, USA). Tests for normality confirmed parametric data sets and the proper use of the paired Student’s t-test.
Lievens C.W., Norgett Y., Briggs N., Allen P.M, & Vianya-Estopa M. (2020). Impact of Improper Approach to Identify Lid Wiper Epitheliopathy (LWE). Clinical Ophthalmology (Auckland, N.Z.), 14, 3039-3047.
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6

Postoperative Tumor Recurrence Assessment

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To assess for recurrence postoperatively, animals underwent laparotomy 12 weeks after surgery, and the tumors were imaged with the Canon EOS 60D digital camera with an EF–S18–55 IS lens (Canon), excised, harvested and weighed for analysis.
Hiroshima Y., Maawy A., Zhang Y., Murakami T., Momiyama M., Mori R., Matsuyama R., Katz M.H., Fleming J.B., Chishima T., Tanaka K., Ichikawa Y., Endo I., Hoffman R.M, & Bouvet M. (2014). Metastatic Recurrence in a Pancreatic Cancer Patient Derived Orthotopic Xenograft (PDOX) Nude Mouse Model Is Inhibited by Neoadjuvant Chemotherapy in Combination with Fluorescence-Guided Surgery with an Anti-CA 19-9-Conjugated Fluorophore. PLoS ONE, 9(12), e114310.
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7

Tumor Treatment Regimens in Mice

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Six weeks after implantation, the mice were randomized to 5 groups and treated as follows: (1) no treatment; (2) carboplatinum (Selleck Chemicals, Houston, TX, USA, 30 mg/kg, ip, weekly, 5 weeks); (3) trastuzumab (Genentech, Inc., South San Francisco, CA, USA, 20 mg/kg, ip, weekly, 5 weeks); (4) S. typhimurium A1-R (5 × 107 CFU/body, ip, weekly, 5 weeks); and (5) S. typhimurium A1-R (5 × 107 CFU/body, ip, weekly, 5 weeks) + trastuzumab (20 mg/kg, ip, weekly, 5 weeks) were co-administered. Each treatment arm comprised 6 tumor-bearing mice. Tumor size was evaluated every 3 or 4 days by caliper measurements and the approximate volume of the tumor was calculated using the formula 4/3π (d/2)2 D/2; where d is the minor tumor axis and D is the major tumor axis. Body weight of the mice was measured on a balance every 3 or 4 days. Relative tumor volume and body weight were calculated by comparison to day-1 values. Tumors were imaged with a Canon EOS 60D digital camera with an EF–S18–55 IS lens (Canon, Tokyo, Japan) and harvested for analysis.
Hiroshima Y., Zhang Y., Zhao M., Zhang N., Murakami T., Maawy A., Mii S., Uehara F., Yamamoto M., Miwa S., Yano S., Momiyama M., Mori R., Matsuyama R., Chishima T., Tanaka K., Ichikawa Y., Bouvet M., Endo I, & Hoffman R.M. (2015). Tumor-Targeting Salmonella typhimurium A1-R in Combination with Trastuzumab Eradicates HER-2-Positive Cervical Cancer Cells in Patient-Derived Mouse Models. PLoS ONE, 10(6), e0120358.
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8

Morphological Analysis of Bolanthus Specimens

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We came across some interesting Bolanthus specimens while conducting field work on the Hasan Mountain above Karkın town (Turkey, Aksaray province), as two authors actually having the goal of finding the Minuartia L. and Dianthus L.. These specimens were compared with related species in the herbarium of Biology Department of Bozok University, GAZI, K and with records in the literature (Barkoudah and Akeroyd 1993 , Zohary 1966 , Huber-Morath 1967 , Huber-Morath et al. 1968 , Davis et al. 1988 , Bojňanský and Fargašová 2007 ). The images were taken using the Canon EOS 60D digital camera, and the seed surface micromorphology was visualized using the LEO 440 scanning electron microscope. Normal visualization of the specimens was carried out using the Olympus SZ61 microscope. The vegetative characters were measured using a ruler with 0.5-mm accuracy and the floral characters were determined using an ocular micrometer.
Koç M, & Hamzaoğlu E. (2015). Bolanthus turcicus (Caryophyllaceae), a new species from Turkey. PhytoKeys.
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9

Microscopic Imaging and Photography

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The specimens were observed with a binocular microscope (Stemi DV4, Zeiss, Oberkochen, Germany) and photographed with a EOS 60D digital camera (Canon, Tokyo) equipped with a macro lens (EF-S60 mm F2.8, Canon, Tokyo). For fluorescent observation, an Axio Zoom.V16 microscope equipped with an AxioCam HRm camera (Zeiss, Oberkochen, Germany) was used. Panorama images of the specimens were produced using Zen 2013 imaging software (Zeiss, Oberkochen, Germany).
Sakata-Haga H., Uchishiba M., Shimada H., Tsukada T., Mitani M., Arikawa T., Shoji H, & Hatta T. (2018). A rapid and nondestructive protocol for whole-mount bone staining of small fish and Xenopus. Scientific Reports, 8, 7453.
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10

Fluorescence-Guided Pancreatic Tumor Resection

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For fluorescence-guided surgery (FGS), a 15-mm transverse incision was made on the left flank of the mouse through the skin and peritoneum and kept open with a retractor. The tail of the pancreas was exposed through this incision. Anti-CEA antibody conjugated to DyLight 650 (50 μg), was injected intravenously via the tail vein in the mice in the FGS group 24 hours before surgery. A MINI MAGLITE® LED PRO flashlight (MAG INSTRUMENT, Ontario, CA, USA) coupled to an excitation filter (ET 640/30X, Chroma Technology Corporation, Bellows Falls, VT, USA) was used as the excitation light source. A Canon EOS 60D digital camera with an EF–S18–55 IS lens (Canon) coupled with an emission filter (HQ700/75M-HCAR, Chroma Technology Corporation) was used as the real-time image capturing device for FGS. BLS was performed under standard bright-field using an MVX10 microscope (Olympus). After completion of surgery, the incision was closed in one layer using 6-0 nylon surgical sutures, and the mice were allowed to recover in their cages.
Hiroshima Y., Maawy A., Zhang Y., Murakami T., Momiyama M., Mori R., Matsuyama R., Chishima T., Tanaka K., Ichikawa Y., Endo I., Hoffman R.M, & Bouvet M. (2015). Fluorescence-guided surgery, but not bright-light surgery, prevents local recurrence in a pancreatic cancer patient derived orthotopic xenograft (PDOX) model resistant to neoadjuvant chemotherapy (NAC). Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.], 15(3), 295-301.
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