Cd41 mwreg30

Manufactured by BD

The CD41 (MWReg30) is a laboratory equipment product manufactured by BD. It is a specialized instrument used for the detection and analysis of specific cellular markers, such as CD41, in biological samples. The core function of the CD41 (MWReg30) is to facilitate the identification and characterization of cells expressing the CD41 antigen, which is commonly associated with platelets. The product provides researchers and clinicians with a tool to study platelet-related processes and disorders.

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Lab products found in correlation

Cd16 32, by Thermo Fisher Scientific (2 mentions) C kit 2b8, by Thermo Fisher Scientific (2 mentions) Sca 1 e13 161.7, by BioLegend (2 mentions) Cd48 hm48 1, by BioLegend (2 mentions) Cd150 tc15 12f12.2, by BioLegend (2 mentions) Cd4 l3t4, by BD (2 mentions) Cd45.1 a20, by BD (2 mentions) B220 ra3 6b2, by BD (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Ve cadherin, by Thermo Fisher Scientific (1 mentions) Ter119, by BD (1 mentions) Gr 1 rb6 8c5, by BD (1 mentions) Cd8 53 6.72, by BD (1 mentions) Cd34 ram34, by BD (1 mentions) Mac 1 m 70, by BD (1 mentions) Flt 3 a2f10.1, by BD (1 mentions) Cy3 cy5 dylight549 dylight649 conjugated iggs, by Jackson ImmunoResearch (1 mentions) Zombie nir, by BioLegend (1 mentions) F4 80 bm8, by BioLegend (1 mentions) Facsaria 2 cell sorter, by BD (1 mentions)

Spelling variants of this product

MWReg30 (20 citations) CD41 (clone MWReg30, (3 citations) Mouse CD41 (clone MWReg30, (2 citations) PE-coupled anti-CD41 antibody (clone MWReg30; (2 citations)

6 protocols using cd41 mwreg30

Immunophenotyping of Hematopoietic Cells

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Primary antibodies used for IHC and flow cytometry were as follows: c-Kit (2B8; eBioscience), CD16/32 (93; eBioscience), VE-cadherin (eBioscience), c-Kit (R&D Systems), Sca-1 (E13-161.7; BioLegend), CD48 (HM48-1; BioLegend), CD150 (TC15-12F12.2; BioLegend), IL-7Rα (SB/199; BioLegend), endoglin (MJ7/18; BioLegend), CD4 (L3T4; BD), CD8 (53-6.72; BD), B220 (RA3-6B2; BD), TER-119 (BD), Gr-1 (RB6-8C5; BD), CD34 (RAM34; BD), Mac-1 (M/70; BD), Flt-3 (A2F10.1; BD), CD41 (MWReg30; BD), CD45.2 (104; BD), CD45.1 (A20; BD), GPIbα (Xia.G5; Emfret), Clec2 (AbD Serotec), and Thpo (Bioss). Secondary antibodies for IHC were Alexa Fluor 488–conjugated IgGs (Molecular Probes) or Cy3/Cy5/DyLight549/DyLight649-conjugated IgGs (Jackson ImmunoResearch Laboratories, Inc.). IHC specimens were treated with DAPI (Molecular Probes) for nuclear staining. Stimulatory rabbit anti–mouse CLEC-2 antibody was a gift of K. Suzuki-Inoue.

Nakamura-Ishizu A., Takubo K., Kobayashi H., Suzuki-Inoue K, & Suda T. (2015). CLEC-2 in megakaryocytes is critical for maintenance of hematopoietic stem cells in the bone marrow. The Journal of Experimental Medicine, 212(12), 2133-2146.

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Kell Antigen Expression Analysis

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For detection of K1 expression, splenocytes, peripheral blood, and platelet-rich plasma were stained with a monoclonal anti-Kell Ab (Mima-8) (58 (link)) followed by anti-mouse IgG and Abs against cell type—specific markers. Mima-8 recognizes the Js^b epitope on the human Kell glycoprotein, which is expressed by the K1 transgene. A combination of Mima-8 and Mima-9, which recognizes Kp^b epitopes of the K1 transgene, was used to compare K1 expression by KEL1A and K1 mice. Platelet-rich plasma was generated by centrifuging peripheral blood at 8000 × g for 10 min. For analysis of dendritic cells (DCs), spleens were minced with a razor blade and filtered through 100 μm Nylon mesh prior to RBC lysis. Single-cell suspensions were stained with fluorescently conjugated Abs specific for cell-surface proteins, including CD19 (Clone: 6D5), TCRβ (H57-597), and I-A/I-E [MHC class II (MHCII), M5/114.15.2], CD86 (GL-1), Ly6C (HK1.4) and F4/80 (BM8) from BioLegend (San Diego, CA); CD45.1 (A20), CD11c (N418), CD11b (M1/70), CD8α (53-6.7), Ter-119, and Siglec H (eBio440c) from eBioscience (San Diego, CA), and CD41 (MWReg30) from BD Biosciences (San Jose, CA). Zombie-NIR (BioLegend) was used to exclude dead cells. Cells were acquired with a Miltenyi MACSQuant flow cytometer and analyzed using FlowJo.

Gibb D.R., Liu J., Natarajan P., Santhanakrishnan M., Madrid D.J., Eisenbarth S.C., Zimring J.C., Iwasaki A, & Hendrickson J.E. (2017). Type I IFN Is Necessary and Sufficient for Inflammation-Induced Red Blood Cell Alloimmunization in Mice. Journal of immunology (Baltimore, Md. : 1950), 199(3), 1041-1050.

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Multiparametric Flow Cytometry Analysis

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All flow cytometric analyses and sorting were performed on a BD FACS Aria II cell sorter. Antibodies used were CD4 (RM4-5, BD Biosciences), CD8a (53-6.7, eBioscience), B220 (RA3-6B2, Biolegend), CD11b (M1/70, BD Biosciences), Ly6G/C (RB6-8C5, Biolegend), Ter119 (TER-119, Biolegend), Sca1 (D7, Biolegend), MPL (AMM2, Immuno-Biological Laboratories), CD41 (MWReg30, BD Biosciences), CD150 (TC15-12F12.2, Biolegend), cKit (2B8, Biolegend), CD34 (RAM34, eBioscience), IL7R (A7R34, eBioscience), Flt3 (A2F10, eBioscience), CD16/32 (93, eBioscience) and CD105 (MJ7/18, Biolegend). Streptavidin (eBioscience) was used to resolve biotinylated antibodies and propidium iodide was added prior to analysis to identify live cells.

O’Neill A., Chin D., Tan D., Majeed A.B., Nakamura-Ishizu A, & Suda T. (2020). Thrombopoietin maintains cell numbers of hematopoietic stem and progenitor cells with megakaryopoietic potential. Haematologica, 106(7), 1883-1891.

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Multiparameter Flow Cytometry Analysis

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The following mAbs were used in this study: rat mAbs against CD16/32 (93; eBioscience), c-Kit (2B8; eBioscience and Tonbo Biosciences), Sca-1 (E13-161.7; BioLegend), CD4 (L3T4; BD Biosciences), CD8a (53-6.72; BD Biosciences), B220 (RA3-6B2; BD Biosciences), TER-119 (TER119; Tonbo Biosciences), Gr-1 (RB6-8C5; BD Biosciences), CD34 (RAM34; eBioscience), Mac-1 (M1/70; BD Biosciences and eBioscience), Flt3 (A2F10.1; BioLegend), CD45.2 (104; BD Biosciences), CD45.1 (A20; BD Biosciences), IL7Rα (SB/119; BioLegend), CD41 (MWReg30; BD Biosciences), CD48 (HM48-1; BioLegend), CD150 (TC15-12F12.2; BioLegend), and CD45 (30-F11; BioLegend and eBioscience and BD Biosciences). A mixture of mAbs against CD4, CD8, B220, TER-119, Mac-1, and Gr-1 was used as a lineage marker (lineage). A mouse anti–Ki67-Alexa Fluor 555 antibody (B56; BD Biosciences) was used to assess the cell cycle, and mouse antiphosphorylated-p38MAPK–phenylephrine (PE) antibody (36/p38; BD Biosciences) and mouse anti-IgG1–PE antibody (BD Biosciences) were used to detect phosphorylated p38MAPK by intracellular flow cytometry. Annexin V–PE (556422; BD Bioscience) was used to analyze apoptosis. For immunocytochemistry, an anti-H2AX (pS139) antibody (N1-431; BD Bioscience), a rabbit anti-53BP1 polyclonal antibody (NB100-304; Novus Biologicals), and Alexa Fluor 555–conjugated anti-rabbit IgG (A-21428; Invitrogen) were used.

Sorimachi Y., Karigane D., Ootomo Y., Kobayashi H., Morikawa T., Otsu K., Kubota Y., Okamoto S., Goda N, & Takubo K. (2021). p38α plays differential roles in hematopoietic stem cell activity dependent on aging contexts. The Journal of Biological Chemistry, 296, 100563.

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Multiparametric Immune Cell Profiling

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Antibodies against F4/80 (BM8) and CD11b (M1/70) were obtained from eBioscience. Antibodies against Ly6G (1A8), CD45 (30-F11), and Ly6C (HK1.4) were from Biolegend. Antibodies against CD49b (clone HMa2), P-selectin (CD62P, clone RB40.34), and CD41 (MWReg30) were purchased from BD Biosciences PharMingen. The MGL1/2 (CD301a/b) and AMR (ASGR1, clone #352803) antibodies, as well as recombinant human MGL (CD301/CLEC10A), were obtained from R&D Systems and labeled with Alexa647 dye using a Microscale Protein Labeling Kit (Thermo Fisher Scientific). Fluorescein-labeled Ricinus Communis Agglutinin (RCA-FITC) was obtained from Vector Laboratories. JON/A-PE antibody used to detect activated integrin αIIbβ3 and X649, an anti-GPIbβ antibody used to label platelets in vivo, were from Emfret Analytics.
ASF, Thiazole Orange, and sialidase (neuraminidase) from Clostridium perfringens were obtained from Sigma-Aldrich. RCA-FITC was obtained from Vector Laboratories. CellTracker Red and Green dyes were from Thermo Fisher Scientific. Clodronate liposomes were from http://www.clodronateliposomes.org.

Deppermann C., Kratofil R.M., Peiseler M., David B.A., Zindel J., Castanheira F.V., van der Wal F., Carestia A., Jenne C.N., Marth J.D, & Kubes P. (2020). Macrophage galactose lectin is critical for Kupffer cells to clear aged platelets. The Journal of Experimental Medicine, 217(4), e20190723.

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Multiparametric Flow Cytometry for Immune Cell Profiling

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Single cell suspensions of spleen were acquired either with LSR II (BD), or MACSQuant (Miltenyi Biotec) flow
cytometers and analyzed using F low Jo software (Tree Star). The following antibodies were used for staining different cell
subsets (from BioLegend unless specified): TCRβ (H57–597), B220 (RA3–6B2), MHC II (M5/114.15.2), CD11c
(N418), 33D1 (33D1), XCR1 (ZET), Va2 (B20.1), CD4 (GK1.5), CD8 (53–6.7), CD19 (6D5), CD23 (B3B4), CD21/35 (7E9), F4/80
(CI:A3–1), CD11 b (M1/70), CD41 (MWReg30, BD Bioscience), IFN-γ (XMG1.2), IL-10Rα (1B1.3a), CD169(SER-4,
eBioscience), Ly6G(1A8).

Liu D., Yin X., Olyha S.J., Nascimento M.S., Chen P., White T., Gowthaman U., Zhang T., Gertie J.A., Zhang B., Xu L., Yurieva M., Devine L., Williams A, & Eisenbarth S.C. (2019). IL-10-Dependent Crosstalk between Murine Marginal Zone B Cells, Macrophages, and CD8α+ Dendritic Cells Promotes Listeria monocytogenes Infection. Immunity, 51(1), 64-76.e7.

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