Conical tube

Manufactured by Merck Group

Sourced in United Kingdom

Conical tubes are a type of laboratory equipment used for a variety of purposes in scientific research and testing. They are designed to hold and contain liquids, solids, or samples during various experimental procedures. The conical shape of the tube allows for efficient mixing and separation of contents. Conical tubes are available in various sizes and materials to suit different needs within the laboratory setting.

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Lab products found in correlation

4 protocols using conical tube

Polystyrene-co-maleic anhydride cell culture

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Polystyrene co-maleic anhydride (molecular weight = ~1600), N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDAC), Hank’s balanced salt solution, Roswell Park Memorial Institute (RPMI) 1640 medium, fetal bovine serum (FBS), bovine serum albumin (BSA), and TrypLE express were bought from ThermoFisher Scientific (Dubai, UAE). L-glutamine and an antibiotic solution of penicillin/streptomycin were purchased from (Merck Hertfordshire, UK). All consumable materials such as Petri dishes, conical tubes (15 mL and 50 mL), cell culture flasks (25 cm² and 75 cm²), and dialysis tubing were purchased from (Merck Hertfordshire, 120 Moorgate London, UK).

El-Deeb I.M., Pittala V., Eltayeb D, & Greish K. (2021). Selective Targeting of Breast Cancer by Tafuramycin A Using SMA-Nanoassemblies. Molecules, 26(12), 3532.

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Cell Culture Reagents and Materials

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Dasatinib were retained from LC Laboratories (Woburn, MA, USA). Polystyrene co-maleic anhydride (molecular weight~1600), Roswell Park Memorial Institute (RPMI) 1640 medium, Hank’s balanced salt solution, fetal bovine serum (FBS), bovine serum albumin (BSA), and TrypLE express were bought from ThermoFisher Scientific (Dubai, United Arab Emirates). N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDAC), L-glutamine, antibiotic solution of penicillin/streptomycin were acquired from (Merck Hertfordshire, UK). All consumable materials including petri dishes, conical tubes (15 mL and 50 mL), cell culture flasks (25 and 75 cm²), and dialysis tubing were purchased from (Merck Hertfordshire, UK).

Bahman F., Pittalà V., Haider M, & Greish K. (2021). Enhanced Anticancer Activity of Nanoformulation of Dasatinib against Triple-Negative Breast Cancer. Journal of Personalized Medicine, 11(6), 559.

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Isolation and Purification of Mouse Brain Cells

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Mice were anesthetized with 10 ug/g of Ketamine/Xylazine mixture and transcardially perfused with 10 mL of cold, sterile PBS (Life Technologies). Brains were digested as previously described.⁵⁷ (link) In brief, brains were isolated and placed into a 50 mL conical tube (Fisher Healthcare) containing 5 mL of RPMI (VWR International LLC). The RPMI containing the brains of the mice were then transferred to a 7 mL glass Tenbroeck Dounce homogenizer (Pyrex) and homogenized until the brain was visibly digested (about 10 plunges). The homogenate was then poured through a 70 μm filter into a new 50 mL conical tube, and 10 more mL of RPMI 1640 was added, along with 1 mL of 10X PBS and 9 mL of Percoll (Sigma-Aldrich INC). The 50 mL conical tubes were then placed into a centrifuge and pelleted at 7840xg for 30 minutes at 4°C. Following the spin, the supernatant was fully aspirated off and samples were washed with 50 mL of fresh RPMI 1640 and spun again at 1500 rpm for 10 minutes. Samples were then blocked in FACS buffer for 15 minutes before surface staining was performed at 4°C for 30 minutes.

Witt L.T., Greenfield K.G, & Knoop K.A. (2024). Streptococcus agalactiae and Escherichia coli induce distinct effector γδ T cell responses during neonatal sepsis. iScience, 27(5), 109669.

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Bacterial Lipid Extraction from M. marinum

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1 liter of M. marinum were grown in GAS medium plus Tween-80 to an OD₆₀₀ of 1.2. Bacteria were pelleted in a glass 50 ml conical tubes (Fisher) of known weight, frozen and lyophilized. The dry bacteria in 50 ml conical tubes were then weighed and the dry bacterial weight was calculated. 25 ml of petroleum ether were then added to the bacteria, and the conical tube was capped with a PTFE lined lid (Sigma) and the suspension was vortexed for 3 min. An additional 25 ml of petroleum ether was added and the sample was centrifuged for 3 min at 1000xg at 4°C. The extract was then saved or discarded depending on the downstream experimental applications and the bacteria were extracted once more as above. For total lipid extractions (not used for recoating), bacteria were treated with 1:1 chloroform:methanol for 12 hr at 60°C. Extracts were filtered, back extracted with water to remove water-soluble contaminants, dried under reduced pressure and used for downstream experiments.

Cambier C., Banik S.M., Buonomo J.A, & Bertozzi C.R. (2020). Spreading of a mycobacterial cell-surface lipid into host epithelial membranes promotes infectivity. eLife, 9, e60648.

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