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Chloramine t

Manufactured by Nacalai Tesque
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Sourced in Japan

Chloramine-T is a chemical compound used as a laboratory reagent. It is a white, crystalline solid with the molecular formula CH3C6H4SO2NClNa. Chloramine-T acts as an oxidizing agent and is commonly used in various analytical and synthetic procedures in chemistry and biology.

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Lab products found in correlation

125i nai, by PerkinElmer (2 mentions) Ang 2, by Fujifilm (1 mentions) Methyl cellulose 400, by Fujifilm (1 mentions) Benzbromarone, by Fujifilm (1 mentions) Dihydroethidium, by Fujifilm (1 mentions) Olmesartan, by Daiichi Sankyo (1 mentions) Isobutylmethylxanthine, by Merck Group (1 mentions) Dulbecco s phosphate buffered saline dpbs, by Thermo Fisher Scientific (1 mentions) Dmem low glucose, by Fujifilm (1 mentions) Diphenyleneiodonium dpi, by Merck Group (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Insulin, by Merck Group (1 mentions) Mitotempo, by Merck Group (1 mentions) Acetic acid, by Nacalai Tesque (1 mentions) Anti f4 80 monoclonal antibody, by Thermo Fisher Scientific (1 mentions) Potassium iodide, by Nacalai Tesque (1 mentions) N acetyl l cysteine, by Nacalai Tesque (1 mentions) Dulbecco s modified eagle medium dmem high glucose, by Fujifilm (1 mentions) High performance liquid chromatography (hplc), by Shimadzu (1 mentions) Cosmosil 5c18 ar 2 column, by Nacalai Tesque (1 mentions)

6 protocols using chloramine t

1

Reagents and Chemicals for Experiments

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Chloramine-T was purchased from Nacalai Tesque Inc. (Kyoto, Japan). Methylcellulose 400, benzbromarone, dihydroethidium (DHE), and ANG II were purchased from Wako Pure Chemical Industries Ltd. (Osaka, Japan). Olmesartan was a kind gift from Daiichi Sankyo Pharmaceutical Co. Ltd. (Tokyo, Japan). All other chemicals were of the highest grade and obtained from commercial sources.
Muraya N., Kadowaki D., Miyamura S., Kitamura K., Uchimura K., Narita Y., Miyamoto Y., Chuang V.T., Taguchi K., Maruyama T., Otagiri M, & Hirata S. (2018). Benzbromarone Attenuates Oxidative Stress in Angiotensin II- and Salt-Induced Hypertensive Model Rats. Oxidative Medicine and Cellular Longevity, 2018, 7635274.
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2

Regulation of Macrophage Oxidative Stress

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Human serum albumin (HSA) was purchased from the Japan Blood Products Organization (Tokyo, Japan). Diphenylene iodonium (DPI), MitoTEMPO, insulin, dexamethasone and isobutylmethylxanthine were purchased from Sigma-Aldrich (St Louis, MO, USA). Anti-F4/80 monoclonal antibody was purchased from eBioscience (San Diego, CA, USA). Potassium iodide, chloramine T, acetic acid and N-acetyl-L-cysteine (NAC) were purchased from Nacalai Tesque (Kyoto, Japan). 5-(and 6)-chloromethyl-2′,7′-dicholorodihydrofluorescein diacetate (CM-H2DCFDA) and Dulbecco’s phosphate-buffered saline (D-PBS) were purchased from Invitrogen (Grand Island, NY, USA). Dulbecco’s modified eagle medium (DMEM)-high glucose and DMEM-low glucose were purchased from FUJIFILM Wako Pure Chemical Co. (Osaka, Japan). All methods were carried out in accordance with approved guidelines. All experimental protocols were approved by Kumamoto University.
Arimura N., Watanabe H., Kato H., Imafuku T., Nakano T., Sueyoshi M., Chikamatsu M., Tokumaru K., Nagasaki T., Maeda H., Tanaka M., Matsushita K, & Maruyama T. (2023). Advanced Oxidation Protein Products Contribute to Chronic-Kidney-Disease-Induced Adipose Inflammation through Macrophage Activation. Toxins, 15(3), 179.
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3

Radiolabeling of L-α-methyltyrosine with 125I

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[125I]NaI (629 GBq/mg as iodine) was obtained from PerkinElmer. No-carrier-added [125I]IMT was prepared using the conventional chloramine-T method from L-α-methyltyrosine as a precursor.Briefly, chloramine-T (Nacalai Tesque) aqueous solution (2 mg/ml) was added to a mixture of L-α-methyltyrosine (1.7 mg/ml) dissolved in 0.1 M phosphate buffer (pH 7.4) and carrier-free [125I]NaI (925 to 1100 kBq). After incubation for 10 min, the reaction was quenched by adding sodium bisulfite aqueous solution (2 mg/ml). [125I]IMT was identified by HPLC (Shimadzu), and IMT, which was prepared using nonradioactive iodine, was identified by mass spectrometry (JMS-T100TD, JEOL). HPLC was performed with a COSMOSIL 5C18-AR-II column (4.5 mm by 150 mm; Nacalai Tesque) at a flow rate of 1 ml/min with a gradient mobile phase of 20% methanol in water with 0.1% trifluoroacetic acid (TFA) to 40% methanol in water with 0.1% TFA for 20 min. The column temperature was maintained at 40°C.
Ozaki K., Yamada T., Horie T., Ishizaki A., Hiraiwa M., Iezaki T., Park G., Fukasawa K., Kamada H., Tokumura K., Motono M., Kaneda K., Ogawa K., Ochi H., Sato S., Kobayashi Y., Shi Y.B., Taylor P.M, & Hinoi E. (2019). The L-type amino acid transporter LAT1 inhibits osteoclastogenesis and maintains bone homeostasis through the mTORC1 pathway. Science signaling, 12(589), eaaw3921.
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4

Radiolabeled Benzamide Receptor Probes

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(S)-(-)-3-trimetylstannyl-2-hydroxy-6-methoxy-N-[(1-ethyl-2-pyrrolidinyl)methyl]-benzamide (TBZM; >95% purity) and S-(-)-IBZM ([127I]IBZM, >95% purity) were purchased from the NARD Institute, Ltd. (Hyogo, Japan) and ABX (Radeberg, Germany), respectively. 125I-NaI (3.3–4.1 GBq/mL) and 123I-NaI (8.5–15.0 GBq/mL) were purchased from PerkinElmer, Inc. (Waltham, MA, USA) and FUJIFILM Toyama Chemical Co., Ltd. (Tokyo, Japan), respectively. Chloramine-T (>95% purity), sodium metabisulfite (>95% purity), and ammonium acetate (>97% purity) were purchased from Nacalai Tesque, Inc. (Kyoto, Japan); domperidone (>90% purity), ethanol (>99.5% purity), and hydrogen carbonate (>95% purity) were provided by FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan).
Mizutani A., Kobayashi M., Ohuchi M., Sasaki K., Muranaka Y., Torikai Y., Fukakusa S., Suzuki C., Nishii R., Haruta S., Magata Y, & Kawai K. (2022). Indirect SPECT Imaging Evaluation for Possible Nose-to-Brain Drug Delivery Using a Compound with Poor Blood–Brain Barrier Permeability in Mice. Pharmaceutics, 14(5), 1026.
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5

Quantification of O-Desmethylvenlafaxine in Biological Samples

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Methanol, chloramine-T, ethylene diamide tetra acetic acid (EDTA), and glucose-6-phosphate (G6P) were purchased from Nacalai tesque (Kyoto, Japan). β-Nicotinamide-adenine dinucleotide phosphate (β-NADP+), sodium hydroxide and isoflurane were purchased from Fujifilm Wako Pure Chemical Industries (Osaka, Japan), and glucose-6-phosphate dehydrogenase (G6PD) was purchased from Oriental Yeast (Osaka, Japan). O-Desmethylvenlafaxine was purchased Tokyo Chemical Industry (Tokyo, Japan). 125I-NaI was purchased from American Radiolabeled Chemicals (St. Louis, MO, USA). 123I-NaI was purchased from Fujifilm Toyama Chemical (Tokyo, Japan). All other reagents and chemicals were of analytical or high-performance liquid chromatography HPLC grade.
Mizutani A., Kobayashi M., Aibe R., Muranaka Y., Nishi K., Kitamura M., Suzuki C., Nishii R., Shikano N., Magata Y., Ishida Y., Kunishima M, & Kawai K. (2022). Measurement of Hepatic CYP3A4 and 2D6 Activity Using Radioiodine-Labeled O-Desmethylvenlafaxine. International Journal of Molecular Sciences, 23(19), 11458.
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6

Serum AOPPs Quantification Protocol

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Serum AOPPs levels were determined by spectrometry (340 nm) as described previously. 37) (link) In a typical expreiment, a 100 µL aliquot serum was diluted ten-fold with phosphate-buffered saline (PBS) transferred to 96-well plate and 20 µL of acetic acid and 10 µL of 1.16 M potassium iodide solution added. A chloramine-T (Nacalai Tesque, Kyoto, Japan) solution was used as the external calibration standard.
Imafuku T., Tanaka M., Tokunaga K., Miyamura S., Kato H., Tanaka S., Nakano T., Hirata K., Kadowaki D., Maeda H., Matsushita K., Otagiri M., Komaba H., Fukagawa M., Watanabe H, & Maruyama T. (2020). Effect of Cinacalcet on the Redox Status of Albumin in Secondary Hyperparathyroidism Patients Receiving Hemodialysis. Biological & pharmaceutical bulletin, 43(10).
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