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2 protocols using western blot antibody diluent

1

Comprehensive Cellular Assay Protocol

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Ag-SP-DNC was synthesized and provided by Tan et al. RPMI-1640 medium were purchased from MACGENE. Fetal Bovine Serum (FBS) were purchased from Corning. Sulforhodamine B (SRB), RNase A, Propidium Iodide (PI) and rat IgG were purchased from Solarbio. FITC AnnexinV Apoptosis Detection Kit and anti-Ki67-PerCP cy5.5 monoclonal antibody were purchased from BD Bioscience. Triton X-100 solution, Ca2+ specific fluorescent probe Fluo-4/AM, JC-1 Staining Kit, Reactive Oxygen Species Assay Kit, GreenNucTM Caspase-3 assay kit, One Step TUNEL Apoptosis Assay Kit were purchased from Beyotime. Western blot Antibody Diluent was purchased from Epizyme. BCA protein assay kit was purchased from Thermo Fisher Scientific. Western BrightTM ECL-Plus was purchased from EMD Millipore. β-actin(13E5) Rabbit mAb were purchased from Cell Signaling Technology. D-Luciferin sodium salt was purchased from YEASEN.
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2

Vitamin C Modulates Cellular Responses

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Vitamin C (purity: > 99.9%) was purchased from Shandong Xinhua pharmaceutical CO., ltd. DMEM Medium, Fetal Bovine Serum (FBS), Wright-Giemsa staining, and Albumin Bovine V were purchased from MACGENE. RBC lysis Buffer, Sulforhodamine B (SRB), RNase A, Propidium Iodide (PI), and rat IgG were purchased from Solarbio. Triton X-100 solution, Ca2+ specific fluorescent probe Fluo-4/AM, JC-1 Staining Kit, One Step TUNEL Apoptosis Assay Kit, and ATP Determination Kit were purchased from Beyotime. FITC Rat Anti-Mouse CD11b was purchased from BD Biosciences. APC Rat Anti-Mouse CD206 was purchased from Miltenyi Biotec. Western blot Antibody Diluent was purchased from Epizyme. Western Bright™ ECL and Western Bright™ Peroxide were purchased from Advansta. Goat Anti-Rabbit IgG (H+L) HRP was purchased from Affinity Biosciences. Vimentin (5G3F10) Mouse mAb, Cleaved Caspase-3 (Asp175) Antibody (Alexa Fluor® 488 Conjugate) and β-actin (13E5) Rabbit mAb were purchased from Cell Signaling Technology. E-cadherin Rabbit PolyAb was purchased from Proteintech.
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