Control or EXDFP knock-down SKOV3 cells (1 × 105/ml) treated with or without various concentrations of paclitaxel were seeded into 6 well plates in a volume of 2 ml culture medium and cultured for 24 h. Cells were stained using an Annexin V and PI staining kit (V13242, Thermo Fisher Scientific, MA, USA) and detected on a FACSCalibur (BD Biosciences). Annexin V and/or PI positive apoptotic cells were analyzed using FlowJo software (FlowJo LLC, OR, USA).
Annexin 5 and pi staining kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Annexin V and PI staining kit is a laboratory tool used to detect and quantify apoptosis, a process of programmed cell death. The kit contains Annexin V, a protein that binds to phosphatidylserine, and propidium iodide (PI), a fluorescent dye that stains DNA. This combination allows for the identification of cells in different stages of apoptosis through flow cytometry analysis.
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2 protocols using annexin 5 and pi staining kit
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Apoptosis Assay of SKOV3 Cells
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Apoptosis Assay of SKOV3 Cells
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SKOV3 cells (1 × 105/ml in a 2 ml per well) were seeded into 6-well plates and cultured overnight. WG-391D was added at increasing concentrations, from 0.2 to 25 μM. Cells were cultured for a further 6 to 24 h and stained using an Annexin V and PI staining kit (V13242, Thermo Fisher Scientific, MA, USA), according to manufacturer's instructions. Apoptotic cells were detected using a FACSCalibur (BD Biosciences) instrument, and analyzed using FlowJo software (FlowJo LLC, OR, USA).
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