Alexa Fluor 488-phalloidin (A12379), Alexa Fluor 568-phalloidin (A12380), and Alexa Fluor 647-phalloidin (A22287) were purchased from Invitrogen. Alexa Fluor 488-goat anti-mouse (A11029), Alexa Fluor 488-goat anti-rabbit (A11034), Alexa Fluor 568-goat-anti-rabbit (A11011), Alexa Fluor 568-goat anti-mouse (A11004), Alexa Fluor 647-goat-anti-mouse (A32728), and Alexa Fluor 647-goat-anti-rabbit (A32733) (1:100) antibodies were purchased from Life Technologies (Grand Island, NY).
The Titin (9D10) antibody, Myomesin (MYOM) antibody, and MYH7 (A4.591) (all 1:2) antibody were purchased from the Developmental Studies Hybridoma Bank (University of Iowa). Mouse anti-α-actinin-2 (1:200, A7811) was purchased from Sigma-Aldrich. Rabbit anti-MYH6 and MYH7 for western blotting (1:500) were purchased from ProteinTech (22281-1-AP, 22280-1-AP).
The Titin (9D10) antibody, Myomesin (MYOM) antibody, and MYH7 (A4.591) (all 1:2) antibody were purchased from the Developmental Studies Hybridoma Bank (University of Iowa). Mouse anti-α-actinin-2 (1:200, A7811) was purchased from Sigma-Aldrich. Rabbit anti-MYH6 and MYH7 for western blotting (1:500) were purchased from ProteinTech (22281-1-AP, 22280-1-AP).