B16F10 mouse melanoma cancer cell line was purchased from ATCC (CRL-6475, Manassas, VA, USA). Ornidazole was obtained from Sigma (cat. no: 16773-42-5, Sigma, Roedermark, Germany) and was used after having been dissolved in Dulbecco's Modified Eagle Medium (DMEM) and distilled water at room temperature for in vitro and in vivo assays, respectively.
Ornidazole
Manufactured by Merck Group
Sourced in United States
Ornidazole is a laboratory chemical used as a standard reference compound for analysis and research purposes. It is a synthetic compound with the chemical formula C13H17Cl2N3O3. Ornidazole is commonly used as a reference standard in various analytical techniques, such as high-performance liquid chromatography (HPLC) and mass spectrometry, to identify and quantify the presence of related compounds in samples.
Automatically generated - may contain errors
Lab products found in correlation
Genistein, by Merck Group (1 mentions)
Helix pomatia, by Merck Group (1 mentions)
β glucuronidase, by Merck Group (1 mentions)
Genistin, by Merck Group (1 mentions)
Sodium oxamate, by Merck Group (1 mentions)
3 brpa, by Merck Group (1 mentions)
Paromomycin, by Merck Group (1 mentions)
Emetine, by Merck Group (1 mentions)
Chloroquine, by Merck Group (1 mentions)
Dnazol reagent, by Thermo Fisher Scientific (1 mentions)
Geneticin g418, by Thermo Fisher Scientific (1 mentions)
N trans epoxysuccinyl l leucine 4 guanidinobutylamide e 64, by Merck Group (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Lipofectamine, by Thermo Fisher Scientific (1 mentions)
Plus reagent, by Thermo Fisher Scientific (1 mentions)
L cysteine, by Merck Group (1 mentions)
4 protocols using ornidazole
1
Ornidazole Effects on B16F10 Melanoma
2
Genistein and Genistin Quantification
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Genistein,
genistin, and β-glucuronidase (EC3.2.1.31, type H-1, 330 000
units/g solid also containing sulfatase activity) from Helix pomatia and ornidazole as internal standards
were purchased from Sigma-Aldrich (St. Louis, MO). The solvents and
reagents for chromatography were purchased from Spectrum (NJ) and
Macron (Hamilton, PA). The standard solutions of Genistein and genistin
were stored in methanol at −20 °C. Triple deionized water
from Millipore (Bedford, MA) was used for all preparations.
genistin, and β-glucuronidase (EC3.2.1.31, type H-1, 330 000
units/g solid also containing sulfatase activity) from Helix pomatia and ornidazole as internal standards
were purchased from Sigma-Aldrich (St. Louis, MO). The solvents and
reagents for chromatography were purchased from Spectrum (NJ) and
Macron (Hamilton, PA). The standard solutions of Genistein and genistin
were stored in methanol at −20 °C. Triple deionized water
from Millipore (Bedford, MA) was used for all preparations.
3
Cell Metabolism Regulation Assay
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ABZ, NTZ, 3-BrPA, ornidazole, CLS, NA-P2, and sodium oxamate were obtained from Sigma-Aldrich (St. Louis, MO, USA). All tissue culture media and fetal calf serum (FCS) were purchased from Hyclone (Logan, UT, USA).
4
Preparation and Storage of Anti-Parasitic Compounds
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MTZ, ornidazole, emetine, chloroquine, paromomycin, L-cysteine, and N-(trans-Epoxysuccinyl)-L-leucine 4-guanidinobutylamide (E-64) were purchased from Sigma-Aldrich (St. Louis, MO, USA), while tinidazole was acquired from LTK Laboratories (St. Paul, MN, USA). DNAzol reagent, TRIzol reagent, PLUS reagent, Lipofectamine, and geneticin (G418) were secured from Invitrogen (Carlsbad, CA, USA). All other chemicals were obtained from Wako Pure Chemical Industries (Osaka, Japan) unless otherwise stated. Drugs were dissolved either in distilled water or dimethyl sulfoxide (DMSO) to a stock concentration of 100 mM and stored at −30°C.
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