Quantum gx μct workstation imaging software

Rats were imaged using a high-resolution micro-computerized tomography (Quantum GX μCT System, PerkinElmer, Waltham) at weeks 6. Visualization and the data of bone volume/total volume × 100% (BV/TV), trabecular number (TbN), trabecular thickness (Tb.Th) and trabecular separation (Tb.Sp) were obtained using the Quantum GX μCT Workstation imaging software (PerkinElmer, Waltham).

Animals were imaged, at weeks 2, 4, and 6, using a high-resolution microcomputerized tomography (μCT) (Quantum GX μCT System, PerkinElmer, Waltham, MA, United States) with 90 kV, 80 μA, and 4.5-μm resolution. Visualization and reconstruction of the data were obtained using the Quantum GX μCT Workstation imaging software (PerkinElmer, Waltham, MA, United States). The volume of interest was defined manually as follows: The cortex area was defined as the region enclosed by the callus and cortical boundaries in tomograms. The trabecular area was an irregular and anatomic region of interest drawn manually, a few voxels away from endocortical surface to medullary space. The cortical pad area (CT. Ar) and cortical pad thickness (CT. Th), BMD, bone volume density (BV/TV, %) and mean thickness of the trabecular (Tb. Th), trabecular number (Tb. N, mm^–1), trabecular separation (Tb. Sp), structure model index (SMI), and bone density of connection (Conn. D, mm^–1) were derived using the Analyze software (AnalyzeDirect, KS, United States).