The determination of TSH levels was carried out using an electrochemiluminescence immunoassay provided by Roche (Mannheim, Germany), with a detectable laboratory range spanning from 0.004 to 100 μIU/mL. The Tg level was assayed using the Elecsys® Tg II kit(Roche Diagnostics, Basilea, Switzerland), with a detection range of 0.04–500.00 ng/mL. The TgAb level was measured using the Elecsys® TgAb kit from Roche Diagnostics, and TgAb levels less than 115 IU/mL were considered negative. Tg was measured with the Elecsys® Tg II kit(Roche Diagnostics, Basilea, Switzerland), with a detection range of 0.04–500.00 ng/mL. Additionally, the TgAb level was assayed using the Elecsys® TgAb kit(Roche Diagnostics, Basilea, Switzerland). TgAb levels falling below 115 IU/mL were deemed negative. Genomic DNA was extracted from the primary tumors and subjected to traditional Sanger sequencing for the identification of BRAFV600E mutations.The BRAFV600E mutation status of primary PTC tumors was determined after surgery.
Elecsys tg 2 kit
Manufactured by Roche
Sourced in Switzerland
The Elecsys® Tg II kit is a laboratory diagnostic tool used for the quantitative determination of thyroglobulin in human serum and plasma. It is designed to provide accurate and reliable measurements of thyroglobulin levels.
Automatically generated - may contain errors
2 protocols using elecsys tg 2 kit
1
Thyroid Cancer Molecular Profiling
2
Comprehensive Thyroid Function Evaluation
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Serum levels of free T3 (fT3), free T4 (fT4), TSH, and thyroglobulin (Tg) were measured with electrochemiluminescence immunoassays (Elecsys FT3 II kit, Elecsys FT4 kit, Elecsys TSH kit, and Elecsys Tg II kit, respectively; Roche Diagnostics, Mannheim, Germany). The reference ranges were 2.33-4.00 pg/mL, 0.880-1.620 ng/dL, 0.500-5.000 μIU/mL, and <33.7 ng/mL respectively. Anti-thyroid peroxidase antibody (TPOAb), anti-thyroglobulin antibody (TgAb), and anti-TSH receptor antibody (TRAb) were measured using Elecsys kits (Roche Diagnostics). The reference ranges were <16 IU/L, <28 IU/L, and <2.0 IU/L, respectively. Measurement of thyroid stimulating antibody (TSAb) titers was performed using a TSAb enzyme immunoassay kit (Yamasa Corp., Chiba, Japan); the cut-off level was 120%.
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