Photometric coolsnap hq2

Epifluorescence microscopy was used to visualize the localization of fluorescent fusion proteins expressing enhanced GFP or red fluorescent protein (RFP) using an IX81 motorized inverted microscope (Olympus Microscopy UK, Southend-on-Sea, UK) equipped with a UPlanSApo 100X/1.40 Oil objective (Olympus). Excitation of fluorescently labeled proteins was carried out using a VS-LMS4 Laser-Merge-System with solid-state lasers (488 nm/50 mW). Laser intensity was controlled by a VS-AOTF100 System and coupled into the light path using a VS-20 Laser-Lens-System (Visitron Systems GmbH, Puchheim, Germany). Images were captured using a Charged-Coupled Device camera (Photometric CoolSNAP HQ2, Roper Scientific). All parts of the system were under the control of the software package MetaMorph (Molecular Devices, Downingtown, PA). High-resolution imaging of M. oryzae Tub2-GFP-expressing transformants was performed using a Leica SP8 laser confocal microscope, with an argon laser line (488 nm) to excite GFP for imaging.

Epifluorescence microscopy to visualize GFP and stained samples was routinely carried out using an IX81 motorized inverted microscope (Olympus) equipped with a UPlanSApo 100X/1.40 Oil objective (Olympus). Excitation of fluorescently-labelled proteins and lipid droplets was carried out using a VS-LMS4 Laser-Merge-System with solid-state lasers (488 nm/50 mW). The laser intensity was controlled by a VS-AOTF100 System and coupled into the light path using a VS-20 Laser-Lens-System (Visitron System). Images were captured using a Charged-Coupled Device camera (Photometric CoolSNAP HQ2, Roper Scientific). All parts of the system were under the control of the software package MetaMorph (Molecular Devices).