Anti cd8 ypt

Mice were infected with 10⁶ PFU ZIKV via intra footpad injection. For bone marrow chimera studies, mice were irradiated at two doses of 475 cGy (total 950 cGy) and reconstituted with 1 × 10⁶ WT or Ifnar1^−/− bone marrow cells. The mice were used for experiments eight weeks after bone marrow transplantation. Chimeras were checked for efficiency of reconstitution through use of congenic bone marrow transfer. Reconstitution was determined to be 91–93% efficient in the Ifnar1^−/− → WT mice, while it was 93–95% efficient within the WT → Ifnar1^−/− mice. For BBB breakdown assay, at 7 DPI with ZIKV or PBS (mock), Oregon green 488-conjugated dextran (70 kDa) (5 mg ml-1, 200 μl per mouse) was injected intravenously. Forty-five minutes later, these mice were sacrificed for immunohistochemical analysis. For depletion studies, Ifnar^−/− mice were injected intravenously with 300 ug of anti-CD4 (GK1.5;BioXCell) and/or anti-CD8 (YPT; BioXCell) antibody at days −1, 4, 9 post ZIKV infection. Depleted mice demonstrated 90–95% efficiency of depletion treatment on day 7 post ZIKV infection. All antibodies for depletion studies were purchased from BioXCell. Mice monitored for clinical illness and were euthanized before reaching the moribund state (onset of hindlimb paralysis (the inability to move hind legs) and/or >20% weight loss) due to humane concerns.