Skov3

Human ovarian cancer cell lines OVCAR3 and SKOV3, and embryonic kidney HEK293T cells were purchased from ATCC (Manassas, VA). Human ovarian cancer cell line IGROV1 was kindly provided by Prof. Silvana Canevari (Istituto Nazionale dei Tumori, Milan, Italy). Human ovarian cancer cell line MES-OV, its carboplatin-resistant derivative MES-OV CBP, and carboplatin-resistant OVCAR3 CBP cells were kindly provided by Dr. Anamaria Brozović (Ruder Bošković Institute, Zagreb, Croatia) (21 (link)). OVCAR3, OVCAR3 CBP, IGROV1, and SKOV3 cells were cultured in RPMI-1640 medium (Euroclone, Milan, Italy), supplemented with 10% fetal bovine serum (FBS; GIBCO, Thermo Fisher Scientific, Waltham, MA), 100 U/mL Penicillin/Streptomycin (Lonza, Basel, Switzerland), 1 mM sodium pyruvate (Lonza), and 2 mM Ultraglutamine (Lonza). Cells were cultured at 37°C, 5% CO₂, and harvested at confluence using trypsin-EDTA (GIBCO). HEK293T, MES-OV, and MES-OV CBP were cultured in DMEM medium (Euroclone) supplemented as described above.

The human cancer cell lines used for the evaluation of the Pt compounds cytotoxicity (see Table 1) were cultured in different culture media, as reported below:

Caco-2: DMEM (low glucose) medium (Sigma-Aldrich, St. Louis, MO, USA), 10% FBS (Sigma-Aldrich, St. Louis, MO, USA), glutamine 2 mM, penicillin (100 U/mL), streptomycin (100 mg/mL), 1% non-essential amino acids.

MG-63 and SK-OV-3: DMEM (high glucose) medium (EuroClone, Pero, MI, Italy), 10% FBS (Sigma-Aldrich, St. Louis, MO, USA), glutamine 2 mM, penicillin (100 U/mL), streptomycin (100 mg/mL).

HeLa, MCF-7 and ZL-55: RPMI 1640 (EuroClone, Pero, MI, Italy), 10% FBS (Sigma-Aldrich, St. Louis, MO, USA), glutamine 2 mM, penicillin (100 U/mL), streptomycin (100 mg/mL).

Hep-G2: DMEM (low glucose) medium (Sigma-Aldrich, St. Louis, MO, USA), 10% FBS (Sigma-Aldrich, St. Louis, MO, USA), glutamine 2 mM, penicillin (100 U/mL), streptomycin (100 mg/mL).

SH-SY5Y: 1:1 mixture of DMEM (high glucose) and Ham’s F-12 Nutrient Mixture (Sigma-Aldrich, St. Louis, MO, USA), 10% FBS (Sigma-Aldrich, St. Louis, MO, USA), glutamine 2 mM, penicillin (100 U/mL), streptomycin (100 mg/mL).