Lysates or immunoprecipitated complexes were electrophoresed on NuPAGE 4–12% Bis-Tris gels (Invitrogen) (Monk and Holding, 2001 (link)) and transferred to polyvinylidene fluoride membrane. Membranes were blocked in Blok-FL Fluorescent blocker (Millipore), then incubated with OCT4 (Abcam 19857, 1:1000 dilution) or DPPA4 (Abnova H00055211-B01, 1:300 dilution) antibody overnight. Blots were incubated with goat anti-mouse (1:10,000 dilution IR Dye 680RD, LiCOR 926–68,070) or goat anti-rabbit secondary antibodies (1:10,000 dilution IR Dye 800CW, LiCOR 926–3211) for 1 h at room temperature and imaged using the Odyssey cLX Imaging system (LiCOR).
Blok fl fluorescent blocker
Manufactured by Merck Group
Blok-FL Fluorescent blocker is a laboratory equipment product designed to reduce unwanted fluorescence in microscopy and imaging applications. It functions by blocking specific wavelengths of light, effectively eliminating background fluorescence and improving the signal-to-noise ratio of the target fluorescent signals.
Automatically generated - may contain errors
2 protocols using blok fl fluorescent blocker
1
Western Blot Analysis of Stem Cell Markers
2
Western Blot Analysis of Stem Cell Markers
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