Chemotaxis assays were performed as described previously [26 (link)]. Bone marrow-cells from WT and AQP9−/− mice were collected and deposited in the upper chamber containing a polycarbonate transwell membrane filter (5-μm pore size; Corning) for 2 h. The lower chamber contained 20 ng/ml CCL2 or 100 ng/ml CXCL12 in 0.5% BSA/RPMI medium. The migrated cells in the lower chamber were analyzed using FACS. The expression levels of monocyte CCR2 and CXCR4 were indicated by fluorescence intensities measured using FACS after staining with anti-CCR2-PE and anti-CXCR4-PE antibodies.
Polycarbonate transwell membrane filter
Manufactured by Corning
The Polycarbonate transwell membrane filter is a laboratory equipment product designed for cell culture applications. It consists of a porous membrane that allows the passage of materials while maintaining a physical barrier between two compartments. The membrane is made of polycarbonate, a durable and transparent material.
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Lab products found in correlation
2 protocols using polycarbonate transwell membrane filter
1
Chemotaxis Assay for Bone Marrow Cells
2
Neutrophil Chemotaxis Assay
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BM-neutrophils from WT and AQP9−/− mice 18 h after DNFB sensitization were deposited on the upper chamber containing a polycarbonate transwell membrane filter (5-μm pore size; Corning) for one hour. The lower chamber contained f MLP (100 nM), CCL19 (200 ng/ml), or CCL21 (200 ng/ml) in 0.5% BSA/RPMI medium. The recovered cells were analyzed with FACS analysis.
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