Aquity uplc beh c18

Manufactured by Waters Corporation

Sourced in United States

The ACQUITY UPLC BEH C18 is a high-performance liquid chromatography (HPLC) column used for the separation and analysis of a wide range of analytes. It features a silica-based stationary phase with C18 alkyl chains, which provides efficient and reproducible separation of compounds. The column is designed to operate at ultra-high pressures, enabling faster analysis times and improved resolution compared to traditional HPLC columns.

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Lab products found in correlation

μ bondasphere c18, by Waters Corporation (2 mentions) Mr hei standard, by Heidolph (1 mentions) Rf 20a, by Shimadzu (1 mentions)

4 protocols using aquity uplc beh c18

Synthesis of PEG-Peptide Conjugates

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Example 2

The synthesis of the PEG-peptide-conjugates for use in the hydrogel self-organization were carried out through Michael-addition-reactions between maleimide-terminal four-armed PEG or maleimide-terminal linear PEG and cysteine-terminal peptides from the library. In the case of star-PEG, both components were dissolved in physiological phosphate buffer solution (1×PBS) with a pH value of 7.4 in a molar ratio of 1:4.5 (star-PEG:peptide) with a total concentration of 80 mg/ml. The reaction mixture was quickly covered and stirred at 750 rpm at room temperature for 18 hours (MR Hei-Standard, Heidolph, Schwabach, Deutschland) The raw products were analyzed through reverse-phase high pressure liquid chromatography (UPLC) (UPLC Aquity™ with UV detector, Waters, Milford, Mass., USA) by using C18 column (AQUITY™ UPLC BEH C18, grain size 1.7 μm, 50×2.1 mM, Waters, Milford, Mass., USA) and an isocratic gradient. The raw product was dialyzed with a dialysis membrane with cut-off limit (cut-off) of 8 kD for two days against 10 liters of water under constant water exchange to release unbound peptides and salt. Thereafter, the product was again injected into the UPLC in order to examine the purity as compared to the analysis before the dialysis. The dialyzed product was dry frozen in water into a solid.

US11166907B2. Non-covalently assembled conductive hydrogel (2021-11-09). DENOVOMATRIX GMBH [DE]. Inventors: Yixin Zhang [DE], Yong Xu [DE], Alvin Thomas [DE].

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Quantification of Salicylic and Jasmonic Acids

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The quantification of SA and SA glucoside (SAG) was performed as described previously with a minor modification (Seo et al. 1995 (link)). Briefly, three leaf disks were frozen and ground using liquid nitrogen. SA and SAG were extracted with 90% methanol, and SAG was converted to SA by β-glucosidase treatment. After separation by HPLC (Shimadzu) with an ODS column (μ-Bondasphere C18, 150 mm�ID3.9 mm, 5 μm, 100A; Waters), SA levels were determined using a fluorescence detector (RF-20A; Shimadzu) with an excitation wavelength of 313 nm and an emission wavelength of 405 nm.
JA quantification was performed as described previously (Kojima et al. 2009 (link), Shinozaki et al. 2015 (link)). Briefly, leaf samples (one leaf per sample) were frozen and ground using liquid nitrogen, and freeze dried. JA was extracted and determined using an ultra-HPLC-Q-Exactive™ system (Thermo Scientific) using an ODS column (AQUITY UPLC BEH C18, 1.7 μm, 2.1�100 mm; Waters) as described (Shinozaki et al. 2015 (link)).

Betsuyaku S., Katou S., Takebayashi Y., Sakakibara H., Nomura N, & Fukuda H. (2017). Salicylic Acid and Jasmonic Acid Pathways are Activated in Spatially Different Domains Around the Infection Site During Effector-Triggered Immunity in Arabidopsis thaliana. Plant and Cell Physiology, 59(1), 8-16.

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Quantifying Soybean Phytohormone Levels

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Phytohormone levels in soybean tissue (100 mg) were quantified as described previously (Kojima and Sakakibara, 2012) using an LC‐MS system (AQUITY UPL System/Xevo‐TQS; Waters, MA, USA) fitted with an octadecylsilyl column (AQUITY UPLC BEH C18, 1.7 μm, 2.1 × 100 mm; Waters). The reproducibility of LC‐MS analysis was assessed with three biological replicates per experiment.

Maruyama K., Urano K., Kusano M., Sakurai T., Takasaki H., Kishimoto M., Yoshiwara K., Kobayashi M., Kojima M., Sakakibara H., Saito K, & Shinozaki K. (2020). Metabolite/phytohormone–gene regulatory networks in soybean organs under dehydration conditions revealed by integration analysis. The Plant Journal, 103(1), 197-211.

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Quantification of Salicylic and Jasmonic Acids

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SA and JA were quantified by high performance liquid chromatography (HPLC) mass spectrometry from crude plant extracts according to the method of Pan et al. [88 (link)]. SA or JA was extracted and quantified using an ultra-HPLC-Q-Exactive^TM system (Thermo Scientific, San José, CA, USA) using an ODS column (μ-Bondasphere C18, 5 μm, 3.9 mm × 150 mm, 100A; Waters, Milford, MA, USA) or an ODS column (AQUITY UPLC BEH C18, 1.7 μm, 2.1 mm × 100 mm; Waters, Milford, MA, USA) as described [88 (link)]. Authentic SA and JA (Sigma Aldrich, Burlington, MA, USA and Olchemim Ltd., Olomouc, Czech Republic) were used as external standards. Three separate biological replicates of each treatment were performed, and each replicate was assessed three times.

Chen Q., Zhang R., Li D, & Wang F. (2021). Integrating Transcriptome and Coexpression Network Analyses to Characterize Salicylic Acid- and Jasmonic Acid-Related Genes in Tolerant Poplars Infected with Rust. International Journal of Molecular Sciences, 22(9), 5001.

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